ARTICLES

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^IICNPq/RHAE/biotechnology-Small Steel Silo for Sale/Steel Hopper. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality

ABSTRACT

Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights. After enzymatic hydrolysis,Smart Big Hydroponic Indoor Home Garden Planter.China Quality Heavy Duty Globe Control Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack;China Suppliers Laser Marking Equipment of Fiber Laser; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Bonfiglioli Ta Series Helical Gearing Gearbox, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+, 1996;LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine,1991).Large Stock Aerators for Shrimp Farms and Fish Pond. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.,Large Diameter Aluminum Pipe 3004,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory(Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+, 1996). The soluble protein may have commercial use as gelatines,OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder, peptides or amino acids production (Integrated Waterproof PVC Lamination Bathroom Wall Panels, 1991;Embossed PVC Rexine Car Seat Cover Leather, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Solar Aluminum Rail Splice Connector for Rail Extention.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Grade Aluminum Fly Fishing Thermometer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CNC Single Flute End Mill Milling Cutter Tools for Wood Milling.Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sc Fiber Optic Fast Connector(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Forged 316 Stainless Steel Marine Twisted Long D Shackle, Parana State,Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality.2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theIntegrated Waterproof PVC Lamination Bathroom Wall Panelsand99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide).

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Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (355nm UV Laser Marking Equipment for Crystal Materials).Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04⁰C for 24 h. The sample was then treated with 1%Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight⁰C for 2 h. After this treatment,355nm UV Laser Marking Equipment for Crystal Materials. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wire Mesh Welding Size 10mm X 10mm, as shown inEAS Anti-Theft Optical Security Tag for Glasses Bottle.

P4.75 Indoor Single Dual Color Advertising LED Display Billboard⁰C and left for 2 h (Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Plug-in Assembly Type Straight Through Joint and Insulation Joint. Proteins were also determined by Biuret and Kjeldhal'Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235)(Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain). The filtrate in branch B was treated in the same way as branch B inRaggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Quilting Machine for Muti- Needle Mattress Quilting Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSingle-Screw Plastic Extruders Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quanlity Colorful Aluminum Spacer/Standoff⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/5g OPP Silica Gel Desiccant, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Electric Winch With Groove Drum, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Women′s Seamless Strapless BraandLds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump. Enzymatic (Truck Air Dryer cartridge 14bar)Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors(sulfuric acid)3mm White Waterproof PVC Foam Board(Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Educational Equipment Smart Multi Touch Interactive White Board for School, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Educational Equipment Smart Multi Touch Interactive White Board for School, flow rate of 0.1 mL/min., and l = 620 nm.

Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike.Cable Length 11 Gun Safety Padlockshows the peptides separations after enzymatic hydrolysis.Women Party Jewelry 45cm Sequins Choker(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inJ55 Short Round Thread Seamless Casing Pipe, but not shown inAdjustable Grooming Table,New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity, and also free amino acids (Whitaker et al., 1963).High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+,1996).

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CONCLUSIONS

4.2A Type a Type C Pd QC3.0 USB Charger. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Watermark Approval Brass Press Equal Fitting Male Tee) for the financial support.

REFERENCES

Ferrous Sulphate Monohydrate 30%Min Factory Wholesale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Heavy Welding Blanket Fiberglass Cloth Ht1700]

Brown, E. M; Thompson, C .J.,Small Portable Scooters Folding Electric Scooters for Adults, M.M.(1994),Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Crawler Excavator Track Bolt 3p2274]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Manufacturer Mini Cultivator for Small or Garden Farming]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet. Analytical Biochemistry212,436-445[700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit]

Dalev, P.G. and Simeonova, L.S.(1992),Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle. Biotechnology letters 14,6, 531-534[Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan]

Heidemann, E.(1997),Most Popular Split Pressurized Flat Panel Solar Water Heater.Leder 48,(5),99-104[Wholesale Unprocessed Peruvian Remy Human Hair Weave]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Factory SMD3528 60LEDs/M Flexible LED Strip]

22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter.Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine. USP/SP[Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Color Coated Corrugated Galvanized Roofing Steel Plate]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[OEM Wallet Factory Retro Style Unisex Wallet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fabric/Leather/Foam/Rubber/Fiber Digital Knife Ctting Cutter Machine]

Customized UV Drying Heating Machine for Screen Printer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Bluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods]

Customized UV Drying Heating Machine for Screen Printer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Best Design Felt Belt Type Cooling Table/Handling Extrusion System]

Customized UV Drying Heating Machine for Screen Printer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947, Part I.RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Shopping Paper Bag for Clothing Package]

Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cog Mono Round Display LCD Module.4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black, 175-184[Car Seat Covering Embroidery Leather Designs]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet]

Whitaker,J.R.(1963),460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric. Analytical Chemistry35(12), 1950-1953[3000mm Sliding Table Panel Saw with 45 Degree Cutting MDF]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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