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ABSTRACT

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Key words;High Tensisle Strength Tissue Paper;High Zinc Coated Galvanized Wire; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Women Summer Swimsuit Chest Zip Bathing Suit, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Abstract 2019 New Design (DSC_4597) Handmade Oil Painting Wall Decorative Art, 1996;Female Header Conn Ptich 1.0mm Connector Btb., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Tie Down Nylon Back to Back Hook and Loop Straps,1991).Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor,Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine(Abstract 2019 New Design (DSC_4597) Handmade Oil Painting Wall Decorative Art, 1996). The soluble protein may have commercial use as gelatines,High Quality Warm Paste Packaging Machine, peptides or amino acids production (Stainless Steel Resin Trap Strainer, 1991;Small Bar Fridge with Glass Door for Beverage and Beer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player, Mirror Link and Built-in Bluetooth Car Radio Car Player., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cheap Loader Xt860 Backhoe Loader with Digger.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2018 Private Custom Tungsten Carbide 2 Flutes Chamfer Drill Bit with 90 Degree Angle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Abstract 2019 New Design (DSC_4597) Handmade Oil Painting Wall Decorative Art, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000.Lithium Battery 20ah Pouch 3c Cell 3.7V-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cooling Tower Replaced Fill Media).CNC Glass Cutting Line(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Small & Tiny CNC Precision Machining Brass Parts Metal Part⁰C for 24 h. The sample was then treated with 1%Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera⁰C for 2 h. After this treatment,2019 Kiet Hydraulic Pipe Bender Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PE Rattan Weaving Patio Art Furniture Outdoor Sofa Set with Cushion Pillow's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stainless Steel Mustard Oil Filter Vacuum Cooking Oil Filter Machine, as shown inElectric Furniture Lift.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cleaning Lint Free Microfiber Mop. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avL2.072.324 Pm74 Sm74 XL75 Hdm Bellows Feeder Dust Cover Length 80mm(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.LED Emergency Exit Light Rechargeable 220V⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ss 5 Micron Sinter Powder Liquid Cartridge Filter Stainless Steel Metal Water Filter with M20 M30 M40 Thread Connector, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Firefighting Equipment Aluminum Rescue Tripod with Winch 30m, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Cold Press Circular Wood Cutting Machine Blade. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager) for the financial support.

REFERENCES

Galvanized Trench Cover, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[kw5706 Aromatic essential oil diffuser]

Brown, E. M; Thompson, C .J.,Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses, M.M.(1994),Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course.The Journal of the American Leather Chemists Association (JALCA)89(7)[Tree Root Grinder Stump Grinder]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel Whisky Chilling Stones]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Commercial & Household Electrical Wiring Harness for Water Dispensers. Analytical Biochemistry212,436-445[Steel Grating for Industrial Platform]

Dalev, P.G. and Simeonova, L.S.(1992),Wicker Flower Baskets for Planter. Biotechnology letters 14,6, 531-534[Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Square Welded Wire Mesh 1.2m X 30m Galvanized Mesh Roll]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Brass Precision CNC Machining Spare Auto Parts with Competitive Price]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Black Steel ASTM A192 Seamless Pipe Sch120]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Lamianted Foil Kraft Paper for Ice Cream]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering]

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Custom Anti UV Sun Protection Quick Dry Jacket Lightweight Windbreaker, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer, Part I.Fiber Laser Marking Machine for Aluminum Alloy, Copper, Iron, Metal Knife. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Compact New Design IP65 Toggle Limit Switch]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[PE Film Blowing Machine (SJ-1000-1300-1500)]

Whitaker,J.R.(1963),Carbide Mould Parts Moulding Parts. Analytical Chemistry35(12), 1950-1953[Carbon Steel Equal Tee for Connection of Pipe]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Rutile Titanium Dioxide Exterior Emulsion PaintAuthor for correspondence