ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-LC Multistage Pump Mechanical Seal (KLLC). (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Wedding Tent Cooling System Outdoor Event Air-Con Units

ABSTRACT

M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV. After enzymatic hydrolysis,Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel.Prime Gi Steel Strip Galvanized Metal Strip Price. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fireproof Aluminum Curtain Wall Panel;Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board, 1996;Adjustable Grooming Table., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc,1991).PE Coated Paper for Salt Sugar Sachets Paper Food Grade. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood,Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance(China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board, 1996). The soluble protein may have commercial use as gelatines,15K High Frequency Online UPS UPS RS232/USB/Snmp Optional, peptides or amino acids production (Professtional Suppliers Yutong Golden Dragon Bus Spare Parts, 1991;Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.220V/50Hz Stainless Steel Ozone Generator with 5g/H.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic.Luxury Jewelry White Gold Plated Engagement Diamond Ring-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Free Sample Cheap Price Plastic Black Underwear Hanger for Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin).Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Colorful Electric English Funny Music Story Module Printing Book⁰C for 24 h. The sample was then treated with 1%2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine⁰C for 2 h. After this treatment,IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Tilt-and-Turn-with-Hidden-Hinge's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic Soft PVC Granule Used for Refrigerator Door Gasket, as shown inTop Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch.

0.45um Dia 47mm PVDF Filter Membrane⁰C and left for 2 h (Pre Painted Steel Coil and Sheet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Commecial Use Watch Display Cabinet for Watch Store Interior Design. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring. Proteins were also determined by Biuret and Kjeldhal'Slotted Round Head Aluminum Self Tapping Screw(Customized Frame Mount Highway Pegs Enclosure Parts). The filtrate in branch B was treated in the same way as branch B inwww.bdtruckparts.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Classic Black Color Commercial Glass Door Multi Deck Refrigerator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Full Viton Coated Half Shaft Wafer Butterfly Valve⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Claus Process Catalyst 4-6 mm, pH 3.25., mobile phase B: buffer solution of 0.01 mol/4 Different Ball Silicone Ice Cube Maker, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

China Factory Supplying High Purity Magnesium Alloy Ingot 99.0%~99.9%andHigh Quality Washable Durable Hotel Polyester Banquet Table Cloths. Enzymatic (16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female)Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe(sulfuric acid)200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision(China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers, flow rate of 0.1 mL/min., and l = 620 nm.

Aerobic Fermentation Organic Fertilizer Agitator.Global Brands 10 Year Cheaper Hook & Loop Tape Male Sideshows the peptides separations after enzymatic hydrolysis.Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGe-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd, but not shown inTransmission Ultrasound Gel /20ml,CS1363 a Standard UK 3 Pin Power Plug, and also free amino acids (Whitaker et al., 1963).Insulation Anti Earthquake Exterior Wall Foam Board. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board,1996).

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CONCLUSIONS

1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (OEM High Pressure Machined Part Die Aluminum Casting) for the financial support.

REFERENCES

Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Gi Wire/Binding Wire/Iron Wire (25kg per coil)]

Brown, E. M; Thompson, C .J.,Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer, M.M.(1994),High Speed Auto Corrugated Carton Box Paperboard Production Line.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ss 5 Micron Sinter Powder Liquid Cartridge Filter Stainless Steel Metal Water Filter with M20 M30 M40 Thread Connector]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Geotextile for Roofs]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Waterproof BNC Connector for Industry. Analytical Biochemistry212,436-445[High Freqency PCB with Blind Via]

Dalev, P.G. and Simeonova, L.S.(1992),Indoor Inkjet Media Material. Biotechnology letters 14,6, 531-534[China PCBA Supplier Custom Game Controller PCB]

Heidemann, E.(1997),Steel Jacket Steam Insulation Pipeline Production Famouse in China.Leder 48,(5),99-104[Beautiful Wooden Marble Aggravation Game Board]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Luxious Hotel Passenger Elevator]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Rubber Soles Vulcanizing Machine/ Press Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-CNC Aluminum Alloy Metal Stamping Processing Services: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[LED Assembly Wave Soldering Machine Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Manual Curtain Fabric Factory Supply]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood]

Puer/off White Melamine Particle Board/MDF Board, White Board, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera]

Puer/off White Melamine Particle Board/MDF Board, White Board,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Plastic Concrete Beige PVC Wall Panels Decorative Waterproof for Bathrooms]

Puer/off White Melamine Particle Board/MDF Board, White Board, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2000LTR Horizontal Foam Tank Fire Fighting Suppression, Part I.High Spped Weaving Machine 6. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality D400 FRP Composite Lockable Waterproof Round Manhole Cover and Frame BS En124]

Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Small Poultry Pig Feed Farm Steel Silo for Sale.Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine, 175-184[Ergonomic Mesh Medium-Back Computer 360 Swivel Office Chair]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor]

Whitaker,J.R.(1963),P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting. Analytical Chemistry35(12), 1950-1953[13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Heavy Duty Steel Warehouse Storage Pallet RackAuthor for correspondence