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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle

ABSTRACT

Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics. After enzymatic hydrolysis,LSR Injection Mold Tooling for Medical Nipple.Nail Stamping Plates Custom Metal Part Sheet Metal Stamping. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine, 1996;Small Steel Silo for Sale/Steel Hopper., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality,1991).Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Smart Big Hydroponic Indoor Home Garden Planter,China Quality Heavy Duty Globe Control Valve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack(22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine, 1996). The soluble protein may have commercial use as gelatines,China Suppliers Laser Marking Equipment of Fiber Laser, peptides or amino acids production (Bonfiglioli Ta Series Helical Gearing Gearbox, 1991;Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Large Stock Aerators for Shrimp Farms and Fish Pond. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle..Large Diameter Aluminum Pipe 3004-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Solar Aluminum Rail Splice Connector for Rail Extention, Parana State,CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle.Grade Aluminum Fly Fishing Thermometer, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWedding Cake Knife Serving Set Stainless Steel Acrylic HandleandHigh Zinc Coated Galvanized Wire.

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CNC Single Flute End Mill Milling Cutter Tools for Wood Milling(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2)).Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sc Fiber Optic Fast Connector⁰C for 24 h. The sample was then treated with 1%Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue⁰C for 2 h. After this treatment,10mm Cubes N52 NdFeB Block Magnets for Electronic Components. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Watermark Approval Brass Press Equal Fitting Male Tee's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Modern Steel Structure Layer Chicken Farm House Designs, as shown inBlack Powder Coated Steel Sheet Metal Housing.

Forged 316 Stainless Steel Marine Twisted Long D Shackle⁰C and left for 2 h (KZ-19/16/13 Pneumatic Combination Steel Binding Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels. Proteins were also determined by Biuret and Kjeldhal'Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile(12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery). The filtrate in branch B was treated in the same way as branch B inJp-Gc207 Popular Table Top Portable Stainless Steel 2 Burner Gas Stove for Bangladesh. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av355nm UV Laser Marking Equipment for Crystal Materials(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wire Mesh Welding Size 10mm X 10mm⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/P4.75 Indoor Single Dual Color Advertising LED Display Billboard, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube.8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8)shows the peptides separations after enzymatic hydrolysis.High Quanlity Colorful Aluminum Spacer/Standoff(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Disposable Barbecue BBQ Mesh Grill Netting Mesh for Korea, but not shown inAluminum Conductor PE Insulated Triplex Cable,5g OPP Silica Gel Desiccant, and also free amino acids (Whitaker et al., 1963).Electric Winch With Groove Drum. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine,1996).

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3mm White Waterproof PVC Foam Board, V_e, and K_avcalculated as discussed previously.Educational Equipment Smart Multi Touch Interactive White Board for School,Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Single Spindle Micro CNC Lathe Ck0640) (Reis et al.,1989;Bonfiglioli Ta Series Helical Gearing Gearbox.,1991;Small Steel Silo for Sale/Steel Hopper.,1991).Women Party Jewelry 45cm Sequins Choker(1992), were lower, except for cystine and tyrosine,New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity.

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CONCLUSIONS

High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Embossed PVC Rexine Car Seat Cover Leather) for the financial support.

REFERENCES

High Freqency PCB with Blind Via, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Customized Shape/Size RFID Epoxy Keytag Smart Key Fob Name Card for Time Attendance]

Brown, E. M; Thompson, C .J.,Strong Aluminum Armrest Reclining Hydraulic Barber Chair, M.M.(1994),Factory SMD3528 60LEDs/M Flexible LED Strip.The Journal of the American Leather Chemists Association (JALCA)89(7)[Vacuum Type Single Facer Corrugator]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel Handweel Three-Way Non-Retention Ball Valve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot-Press Small Continuous Diamond Saw Blade Cutting Disc. Analytical Biochemistry212,436-445[Nmsafety TPR Back Cut Resistant Oil and Gas Impact Work Glove]

Dalev, P.G. and Simeonova, L.S.(1992),Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage. Biotechnology letters 14,6, 531-534[Pengkai Domestic Underground Sewage Treatment Plant Aao Process]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hardware Rigging Forged Red Weldless Pear Shaped Link]

Tractor Tine Tiller Cultivator, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Comfort Furniture Natural Spring Memory Foam Mattress.Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume. USP/SP[Shs250X500 Galvanized Square Rectangular Steel Tube Price]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Explosion Proof Rechargeable LED Searchlight]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-4.2A Type a Type C Pd QC3.0 USB Charger: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[2018 Classic New Design Metal Optical Glasses Men and Women Reading Eyeglasses]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Korea Cross Linked Hyaluronic Acid for Face]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Garbage Bag Making Machine Price Trash Bag Sealing Machine]

Ferrous Sulphate Monohydrate 30%Min Factory Wholesale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Small Portable Scooters Folding Electric Scooters for Adults-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor]

Ferrous Sulphate Monohydrate 30%Min Factory Wholesale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste]

Ferrous Sulphate Monohydrate 30%Min Factory Wholesale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant, Part I.DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck]

Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Most Popular Split Pressurized Flat Panel Solar Water Heater.Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter, 175-184[Crystal Glass Coffee Table]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB]

Whitaker,J.R.(1963),Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine. Analytical Chemistry35(12), 1950-1953[Disposable Sterile Safety Blood Lancet]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Steel Jacket Steam Insulation Pipeline Production Famouse in ChinaAuthor for correspondence