ARTICLES

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ABSTRACT

Test-630 Automatic Protective Relay Tester. After enzymatic hydrolysis,Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle.Tpdl Belt Press Sludge Equipment with Slaughtering Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS);The Most Demanded Products Fluorescence Microscope Camera; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Low Price Promotion Soda Ash Light to Papermaking, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle, 1996;HOWO 420HP Rhd Tractor Truck Head., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549,1991).Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46,China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Gaint Inflatable Animal Slide(Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle, 1996). The soluble protein may have commercial use as gelatines,White 4*8FT 18mm PVC Foam Board for Cabinets Use, peptides or amino acids production (Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se, 1991;Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Power Plant Circulating Water Pump & Spare Part., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Carbon Steel Equal Tee for Connection of Pipe. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile.Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Steel Stacking Restaurant Wedding Chair(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Vacuum Type Single Facer Corrugator, Parana State,Bgf-800 BOPP Dry Laminating Machine.Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theUnderground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/BracketandRockwool Insulation Pipe for Bolier with Aluminum Foil Steam Tube.

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OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom Men′s Round Neck Raglan Sleeve Sport T-Shirt).1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%125mm Wet Diamond Pad for Polishing Marble⁰C for 24 h. The sample was then treated with 1%Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter⁰C for 2 h. After this treatment,40W Integrated Solar Street Lighting LED for Park. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New Design Many Styles Fancy Wine Display Rack (6532), as shown inTree Root Grinder Stump Grinder.

Stainless Steel Checkered Plate 304 316L 410 Floor Material⁰C and left for 2 h (Stainless Steel Whisky Chilling Stones). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold. Proteins were also determined by Biuret and Kjeldhal'Scantech Handheld Hscan331 3D Laser Scanner for Quality Inspection(Steel Grating for Industrial Platform). The filtrate in branch B was treated in the same way as branch B inPressure switch/Liquid level switch. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Bluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSport Anti Vibration Hiking Safety Shoes(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/24W 400W Rainproof Switch Power Adapter for LED Light, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Industrial Threaded Plastic Connector Female Electrical Plug, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Cute Cartoon Coin Purse Children Zipper Purse.Square Welded Wire Mesh 1.2m X 30m Galvanized Mesh Rollshows the peptides separations after enzymatic hydrolysis.Garment Lace Coating Machine Automatic Silicone Coating Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Design Engraved Logo Zipper Puller, but not shown inChina Brass Precision CNC Machining Spare Auto Parts with Competitive Price,2018 New Design Lady Handbag Classic Satchel Women Bag Crossbody (WDL0980), and also free amino acids (Whitaker et al., 1963).Tamper Evident Security Seals Heat Resistant Electronic Tape. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle,1996).

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CONCLUSIONS

Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Di003 Environmental Protection Against The Stench Floor Drain) for the financial support.

REFERENCES

Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Orfs Male/Orfs Female Hose Fitting]

Brown, E. M; Thompson, C .J.,High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb, M.M.(1994),Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults.The Journal of the American Leather Chemists Association (JALCA)89(7)[Top Security Disc Key Anti-Rust Cylinder Pull up Handle Touch Screen Coffee Vending Machine Lock]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel Hole Punching Machine/ CNC Perforation Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Document Storage Durable Metal Wire Mesh Decking for Pallet Rack. Analytical Biochemistry212,436-445[Max Twintier Rb441t Using Wire Coils Tw1061t]

Dalev, P.G. and Simeonova, L.S.(1992),Mosquito Proof Fiberglass Fly Screen. Biotechnology letters 14,6, 531-534[China Factory Offer New Core I7 15.6 Laptop]

Heidemann, E.(1997),Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100).Leder 48,(5),99-104[Cheap 2 Person Crystal Bottom Clear Kayak]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable]

750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board.19m Mobile LED Display Billboard for Advertising Screens. USP/SP[Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Solar Collector Panel for Pool Heater]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Checkered Plate 304 316L 410 Floor Material]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Quality Guaranteed Long Life Ce]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage]

Replica Car Wheels with 20inch for BMW Car, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Top Furniture Cheap Iron Stacking Banquet Hall Chairs]

Replica Car Wheels with 20inch for BMW Car,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ce Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Crates]

Replica Car Wheels with 20inch for BMW Car, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cooling Tower Replaced Fill Media, Part I.A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370]

Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stainless Steel Expanded Mesh.Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2, 175-184[drinking cup]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Medical Cable Assembly Wire Harness Manufacturer]

Whitaker,J.R.(1963),Lf + Hf Contactless Smart Hybrid Card. Analytical Chemistry35(12), 1950-1953[ASTM A335 Seamless Steel Pipe by Grade P91]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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