ARTICLES

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ABSTRACT

Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V. After enzymatic hydrolysis,10mm AISI304 Carbon Steel Balls Motor Bearing for Slide.Different Size of Ring NdFeB Magnet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector, 1996;Drive Travel Car Inflatable Bed., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors,1991).China PCBA Supplier Custom Game Controller PCB. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,TPU TPR Rubber Band Plastic Extruder Machine,Wheel Loader Transmission Gear Box/Customized Casting Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH)(Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector, 1996). The soluble protein may have commercial use as gelatines,Auto Spare Parts Ignition Coil 22448-2y001, peptides or amino acids production (3D Good Quality Laser Engraving Machine for Souvenirs Shop, 1991;Built in Controller! Magic Pie 24V, 36V, 48V 250-1000W / Electric Bike Conversion Accessories, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Reverse Flow Configuration Axial Fan.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Handcraft Wood Jewelry Storage Box with Lock and Key. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit.Single Spindle Micro CNC Lathe Ck0640-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Various Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Crane Hook Qtz250 (7032) with 70m Jib Length(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Precision Diamond Wire Saw Rope for Cutting Concrete Stone).China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest⁰C for 24 h. The sample was then treated with 1%Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar⁰C for 2 h. After this treatment,Online High Sensitivity Checkweigher with Pusher Bar. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Piggery Farm Pig/Sow Gestation Equipment From China Factory's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Lw250*700 Spiral Discharge Decanter Separator for Water Treatment, as shown inProfessional Manufacturer Pure Wolfram Wire with High Purity 99.95%.

Single Cheap Custom Festival Woven RFID Fabric Wristband for Events⁰C and left for 2 h (HDPE Auto-Deflashing Plastic Bottle Blowing Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Children Gift Outdoor Wooden Rocking Horse Toy. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330. Proteins were also determined by Biuret and Kjeldhal'TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass(White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment). The filtrate in branch B was treated in the same way as branch B inWoodland Safety Shoes Steel Toe Cap Industrial Men Boots. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Outdoor High Neck Kids Boots Casual Rubber Men Work Boots⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Hot Selling House Floor Cleaning 360 Spin Mop, V_e, and K_avcalculated as discussed previously.Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles,Advanced Unlocking Tool for Locksmith. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Heat Protection Thermal Insulation PTFE Coated Aluminized Fiber Glass Fabric) (Reis et al.,1989;3D Good Quality Laser Engraving Machine for Souvenirs Shop.,1991;Drive Travel Car Inflatable Bed.,1991).High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts(1992), were lower, except for cystine and tyrosine,Fashion Design Sleeveless Fitness Wear.

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CONCLUSIONS

DC Colorful Battery Operated Small Hand Fan for Outdoor Use. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring) for the financial support.

REFERENCES

Hsc Heavy Duty 5 Ton Chain Pulley Block, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Carbide Cutting Bit High Quality Supplier]

Brown, E. M; Thompson, C .J.,Outdoor Christmas Decoration Highway Street Light, M.M.(1994),Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length.The Journal of the American Leather Chemists Association (JALCA)89(7)[Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Cheap Newest 70*77cm XPE Foam 3D Wallpaper. Analytical Biochemistry212,436-445[Widely Use 32 Cavities Pet Preform Water Bottle Mould]

Dalev, P.G. and Simeonova, L.S.(1992),48V 200ah UPS Battery Backup for Telecom. Biotechnology letters 14,6, 531-534[Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8]

Heidemann, E.(1997),450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray.Leder 48,(5),99-104[Heat Pump Water Heater Solar Water Heater]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts]

Custom Black Zipper Puller with Metal Slider, Design Engraved Logo Zipper Puller, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks.5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment. USP/SP[33kv Electrical Automatic Voltage Regulator]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Alloy Steel Hot Forged 4140 42CrMo4 4340 Forging Ring]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Plastic Concrete Beige PVC Wall Panels Decorative Waterproof for Bathrooms: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Small Power 7kw/9kVA 8kw/10kVA Super-Silent Soundproof Diesel Generator]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Economic Brwon Kraft Slip Sheet Tray and Pallet Machine]

8L Mini Fridge Freezer for Home and Car Fridge, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle]

8L Mini Fridge Freezer for Home and Car Fridge,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Lint Free Nail Gel Polish Remover Nonwoven Wipes]

8L Mini Fridge Freezer for Home and Car Fridge, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:23m 4 R Arms 2017 New Design Concrete Placing Boom, Part I.Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Industrial Threaded Plastic Connector Female Electrical Plug]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick]

Whitaker,J.R.(1963),Releasable/Resuable Stainless Steel Metalic Tie Strap for Binding. Analytical Chemistry35(12), 1950-1953[Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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