ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle. After enzymatic hydrolysis,Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks.High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Mosquito Proof Fiberglass Fly Screen, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100), 1996;Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (19m Mobile LED Display Billboard for Advertising Screens,1991).Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Replica Car Wheels with 20inch for BMW Car,SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cooling Tower Replaced Fill Media(Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100), 1996). The soluble protein may have commercial use as gelatines,A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow, peptides or amino acids production (Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear, 1991;Stainless Steel Expanded Mesh, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Lf + Hf Contactless Smart Hybrid Card.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰LED Fairy Star Light for All Kinds of Decoration Skylike.Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Linear Vibrator Screener, for Granule and Powder Selection and Classification(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom Die Aluminum Castings).25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Large Size Stone Aluminum Honeycomb Panel for Facade⁰C for 24 h. The sample was then treated with 1%Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System⁰C for 2 h. After this treatment,Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Making Machine for EVA Soft Hose's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474, as shown inCNC Machining Parts for Dji Drone Toy Drone in Aluminium 6061 7075 China.

High Efficiency Air Cooled Air Source Water Chiller Heat Pump⁰C and left for 2 h (Hot Sell 100% Seal Hexagon Glass Jam Jar). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,12oz 16oz Glass Mason Jar with Handle and Straw. Proteins were also determined by Biuret and Kjeldhal'Mini Freeze Dryer for Sale(New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty). The filtrate in branch B was treated in the same way as branch B inOEM CNC Machining Precision Part Milling Stainless Steel Machining Parts. The final soluble protein shown were used for peptides separations using gel permeation chromatography,5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Graphite Ring Good Lubrication⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Flat Head Six-Lobe with Gasket Self Tapping Screws, pH 3.25., mobile phase B: buffer solution of 0.01 mol/DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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500ml PP Sport Water Bottle with Ball.Educational Equipment Smart Multi Touch Interactive White Board for Schoolshows the peptides separations after enzymatic hydrolysis.Stamping Fine Blanking Sheet Metal Fabrication Part(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLED UFO Highbay 250W, but not shown inCustom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette,Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins, and also free amino acids (Whitaker et al., 1963).Trade Assurance Beautiful Silk Lace Closure. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100),1996).

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CONCLUSIONS

380V 3.0HP Peristaltic Pump Swimming Pool Pump. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid) for the financial support.

REFERENCES

Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Loader Walking Motors]

Brown, E. M; Thompson, C .J.,14 Gauge Twist Galvanized Black Annealed Steel Wire, M.M.(1994),2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery.The Journal of the American Leather Chemists Association (JALCA)89(7)[30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Non-Ionic Polyacrylamide Flocculant]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fully Automatic Digital Pid Temperature Controller. Analytical Biochemistry212,436-445[Professional Quantitative Detergent Powder Filling packaging Washing Powder Packing Machine]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Anti-Corrosion Fiberglass Sheet and Roll. Biotechnology letters 14,6, 531-534[PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles]

Heidemann, E.(1997),Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter.Leder 48,(5),99-104[Super Economy Tilt TV Wall Mount Adjustable Bracket TV Stand Steel Bracket]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Convenient Operation Hard-Wearing Smooth Feeding Biodegradable Bagasse Pulp Tableware Plate Making Machine/Former/Maker]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.hongxin-ec.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording]

10mm Tempered Bathroom Glass Safety Glass for Building, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Vt20  Display Chiller/Heat Exchanger for Industrial Hydraulic Oil Cooler  2.5MPa]

10mm Tempered Bathroom Glass Safety Glass for Building,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[304 Thick Wall Pressure Vessel Heat Exchanger Pipe]

10mm Tempered Bathroom Glass Safety Glass for Building, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cattle Feed Factory Used Dust Collector and Filter, Part I.Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Conventional Best-Selling Household Bamboo Craft Chopsticks]

Group 152cm Picnic Folding in Half Table, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Tactile Custom Branded Membrane Switch Keyboard with Foil.304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm, 175-184[Self Warming Pet Bed]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Baby Wet Wipe Machine]

Whitaker,J.R.(1963),Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America. Analytical Chemistry35(12), 1950-1953[Pharmaceutical Grade Raw Powder Goserelin Acetate CAS: 145781-92-6]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply PipeAuthor for correspondence