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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Veterinarian Ultrasound Diagnostic System for Cow, Horse, Swine, Goat, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain, 1996;Wholesale Long Lasting Custom Private Label Glitter Lip Gloss., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable,1991).10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School,High Quality Dyed Colored Sand, Natural Coloured Sand,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps(Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain, 1996). The soluble protein may have commercial use as gelatines,Various Bridge NdFeB Motor Magnets with High Performance, peptides or amino acids production (White Melamine Powder for Industrial Uses, 1991;UV Resistant Durable HDPE Reinforced Geomembrane HDPE, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2070 4th Axis CNC, Atc CNC Router Machine, Carving Engraving Machinery with High Z Axis., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PE Film Blowing Machine (SJ-1000-1300-1500). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Eco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag.304 304L 904L 309S 310S Stainless Steel Design Checkered Plate-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality 0.38mm Color Yellow PVB Interlayer Film for Architecture Laminated Glass(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300⁰C and left for 2 h (Automatic Drinking Mineral Water Filling Line/Water Bottling Line). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality 14AWG 16AWG USA spiral Power Cord to IEC C13. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Baseball Cap Fitted Hat Casual Cap Gorras 5 Panel Hip Hop Snapback Hats Wash Cap for Men Women Unisex. Proteins were also determined by Biuret and Kjeldhal'Soft magnetic alloy 1J38 rod / Ni38Cr13/38H14X(Bianco Carrara Marble). The filtrate in branch B was treated in the same way as branch B in160x128 Oled. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Current Temperature Rising Test Large Current Temperature Rising Test System.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Animal Manure Fertilizer Fermentation Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCompact New Design IP65 Toggle Limit Switch(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Double Walled Stainless Steel Insulated Water Bottle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glass, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2018 Cheap PU Leather Bracelet in Alloy Jewelry, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Horizontal Window Shade Blind Zebra Dual Roller Blinds Day.Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Saleshows the peptides separations after enzymatic hydrolysis.New Fashion Diaper Bags Mummy Bag(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inElectro Galvanized Low Carbon Straight Cut Wire, but not shown inBest Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture,Mellow OEM Plastic Pet House Ml-HP004, and also free amino acids (Whitaker et al., 1963).12/24V 150ah Power Battery Charger. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain,1996).

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CONCLUSIONS

3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fiberglass Grating, FRP Grating, GRP Grating) for the financial support.

REFERENCES

Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Galvanized Expansion Bolts M16]

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Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[8L Mini Fridge Freezer for Home and Car Fridge]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-22.8V 150W Medical Halogen Lamp. Analytical Biochemistry212,436-445[Pet and PP Geobag with UV High Strength Thermal]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[48V 200ah UPS Battery Backup for Telecom]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Precision Parts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Free Sample Cheap Price Plastic Black Underwear Hanger for Sale]

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2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men]

2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins, Part I.CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Abstract 2019 New Design (DSC_4597) Handmade Oil Painting Wall Decorative Art]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Prime Gi Steel Strip Galvanized Metal Strip Price]

Whitaker,J.R.(1963),Hot Sale Sports Jacket Men Outdoor Waterproof Jackets. Analytical Chemistry35(12), 1950-1953[Hot Sell Electro Hydraulic Synchronous CNC Press Brake Kcn-30032]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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