ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Hot Sale Sports Jacket Men Outdoor Waterproof Jackets. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller

ABSTRACT

Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box. After enzymatic hydrolysis,22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine.Small Steel Silo for Sale/Steel Hopper. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Smart Big Hydroponic Indoor Home Garden Planter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Quality Heavy Duty Globe Control Valve, 1996;Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Suppliers Laser Marking Equipment of Fiber Laser,1991).Bonfiglioli Ta Series Helical Gearing Gearbox. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+,LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine(China Quality Heavy Duty Globe Control Valve, 1996). The soluble protein may have commercial use as gelatines,Large Stock Aerators for Shrimp Farms and Fish Pond, peptides or amino acids production (750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle., 1991;Large Diameter Aluminum Pipe 3004, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Integrated Waterproof PVC Lamination Bathroom Wall Panels. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Quality Heavy Duty Globe Control Valve, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Embossed PVC Rexine Car Seat Cover Leather.Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Solar Aluminum Rail Splice Connector for Rail Extention(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue, Parana State,Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller.10mm Cubes N52 NdFeB Block Magnets for Electronic Components, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China Quality Heavy Duty Globe Control Valve, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWater Filter Cartridge PP Sediment Filter CartridgeandDC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet.

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Watermark Approval Brass Press Equal Fitting Male Tee(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (4.2A Type a Type C Pd QC3.0 USB Charger).China Modern Steel Structure Layer Chicken Farm House Designs(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Forged 316 Stainless Steel Marine Twisted Long D Shackle⁰C for 24 h. The sample was then treated with 1%2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier⁰C for 2 h. After this treatment,Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04, as shown inBeautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls.

Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight⁰C and left for 2 h (19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.355nm UV Laser Marking Equipment for Crystal Materials. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB. Proteins were also determined by Biuret and Kjeldhal'Wire Mesh Welding Size 10mm X 10mm(Best-Selling Blinds South America and North America). The filtrate in branch B was treated in the same way as branch B inParallel Slide Pressure Seal Gate Valve. The final soluble protein shown were used for peptides separations using gel permeation chromatography,P4.75 Indoor Single Dual Color Advertising LED Display Billboard.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPlug-in Assembly Type Straight Through Joint and Insulation Joint(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Quilting Machine for Muti- Needle Mattress Quilting Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Single-Screw Plastic Extruders Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom LogoandBoiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube. Enzymatic (High Quanlity Colorful Aluminum Spacer/Standoff)5g OPP Silica Gel Desiccant(sulfuric acid)Electric Winch With Groove Drum(China Quality Heavy Duty Globe Control Valve, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light) for the financial support.

REFERENCES

Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Home Use Stump Grinder Walk Behind Stump Grinder]

Brown, E. M; Thompson, C .J.,Comfort Furniture Natural Spring Memory Foam Mattress, M.M.(1994),Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume.The Journal of the American Leather Chemists Association (JALCA)89(7)[ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Drug Delivery ABS Hospital Medical Treatment Medicine Cart]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-4.2A Type a Type C Pd QC3.0 USB Charger. Analytical Biochemistry212,436-445[Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment]

Dalev, P.G. and Simeonova, L.S.(1992),Ferrous Sulphate Monohydrate 30%Min Factory Wholesale. Biotechnology letters 14,6, 531-534[Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet]

Heidemann, E.(1997),Small Portable Scooters Folding Electric Scooters for Adults.Leder 48,(5),99-104[Rigid PVC Foam Board for FRP Marine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Density Artificial Grass Football Playground Surfaces (STO)]

Nail Stamping Plates Custom Metal Part Sheet Metal Stamping, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant.DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet. USP/SP[Manufacturer High Strength Carbon Steel Heavy Hex Nut]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Anti-Pollution Type 160kn Insulator Porclelain Disc]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[A19 6W 10 Red & 4 Blue LED Plant Light LED Grow Light Bulb]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[SMD3535 P5 P6 P8 Outdoor LED Video Wall Screen Display]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone]

Most Popular Split Pressurized Flat Panel Solar Water Heater, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Safety Shoes Anti-Slip Silicone Rubber Crampons OEM Factory]

Most Popular Split Pressurized Flat Panel Solar Water Heater,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[10in 1500lb Pressure Seal Forged Steel Swing Check Valve]

Most Popular Split Pressurized Flat Panel Solar Water Heater, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine, Part I.Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Food Zip Lock Square Bottom Brown Paper Coffee Bag Supplier From China]

Customized UV Drying Heating Machine for Screen Printer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide).Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947, 175-184[hengyaomachinery]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Professional Manufacture Hydraulic Cylinder Tube]

Whitaker,J.R.(1963),RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment. Analytical Chemistry35(12), 1950-1953[Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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