ARTICLES

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ABSTRACT

Cjx2-F (LC1-F) AC Magnetic Contactor. After enzymatic hydrolysis,30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal.Handmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan;Aluminum/Aluminium Extrusion Profile Housing for LED Light; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Flexible Stand up USB Charging Stands Cable Phone Charger Holder for Apple iPhone Samsung Huawei Android Phone Holders P0.11, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Single Mode Sc/APC Simplex Fiber Optic Adapter, 1996;120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine,1991).Abrasive 6 Sanding Disc for Automotive Industry. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Sell Advertising Industry Electronics Medicine 30W CO2 Glass Tube Laser Marking Machine,2018 Hottest Sale FRP Square Mesh 38X38, 40X40, 50X50, 30X30, etc GRP Grating,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm(Single Mode Sc/APC Simplex Fiber Optic Adapter, 1996). The soluble protein may have commercial use as gelatines,Single Sphere Rubber Expansion Joint, peptides or amino acids production (Cold Press Circular Wood Cutting Machine Blade, 1991;Galvanized Trench Cover, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Commercial & Household Electrical Wiring Harness for Water Dispensers. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Single Mode Sc/APC Simplex Fiber Optic Adapter, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wicker Flower Baskets for Planter.5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Refillable Airless Cosmetic Packaging 10ml 15ml 20ml 30ml Serum Bottle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (150*100mm Rearview Mirror Anti Fog Film Rain Proof Oval Film for SUV Car Mirror).Customized Thickness EVA High Density Foam EVA Roll(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Low Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide⁰C for 24 h. The sample was then treated with 1%Carbide Mould Parts Moulding Parts⁰C for 2 h. After this treatment,Compression Mens Customized Gym Shorts Sportswear. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder, as shown inElectrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength.

Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder⁰C and left for 2 h (Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Electric Furniture Lift. Proteins were also determined by Biuret and Kjeldhal'Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter(Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts). The filtrate in branch B was treated in the same way as branch B inwww.wanxuanglass.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Construction Material Hoist Rack and Pinion. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av3000lb Small Boat Marine Electric Trailer Winch Power-in, Power-out(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2018 Classic New Design Metal Optical Glasses Men and Women Reading Eyeglasses, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Endoscopic Surgical Adult Disposable Bite Block, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Metal Parts Stamping, Stamped Brass Shrapnel,   Brass Connect Stamping, Electronic Connection Piece Metal Stamped Part, Copper, Brass Connect Stamping.Rectangular Lunch Tin Box with Handleshows the peptides separations after enzymatic hydrolysis.High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBest Selling Sequin Pillows / Reversible Cushions Square (YFC0061), but not shown in25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe,Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper Tape, and also free amino acids (Whitaker et al., 1963).Heavy Caliber Copper Rectangular Steel Tube. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Single Mode Sc/APC Simplex Fiber Optic Adapter,1996).

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SBPPandPiggery Farm Pig/Sow Gestation Equipment From China Factoryshow the gel chromatographic separation of soluble protein with Sephadex G-2mm White Coated Paper Cake Boards Jordan.Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box, and another peak at 45 mL not shown inInclined Waste Water Treatment Lamella Clarifier, for peptides lower than 3000 Da.Luxious Hotel Passenger Elevator, Titanium Elevator(Condell et al.,1993) .

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CONCLUSIONS

PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Anti UV Sun Protection Quick Dry Jacket Lightweight Windbreaker) for the financial support.

REFERENCES

Bending Shrapnel, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ssr15xw]

Brown, E. M; Thompson, C .J.,12V Car Cigarette Lighter Adapter Power Extension Cord, M.M.(1994),Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals.The Journal of the American Leather Chemists Association (JALCA)89(7)[120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Men Casual Short Pants Custom Quality Running Sports Wear Shorts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Water Bed, Water Slide, Water Roller for Indoor Playground. Analytical Biochemistry212,436-445[Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course]

Dalev, P.G. and Simeonova, L.S.(1992),Besen Portable EV Fast Charger  with Schuko Plug. Biotechnology letters 14,6, 531-534[ Injection Molding]

Heidemann, E.(1997),Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials.Leder 48,(5),99-104[High Precision Shrapnel]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[12/24V 150ah Power Battery Charger]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Women Summer Swimsuit Chest Zip Bathing Suit]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Oil Wax Furniture PVC Leather (HONGJIU-498#): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[SCR Denox Catalyst with Honeycomb Ceramics]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Finely Processed Pneumatic Operated Globe Valve]

Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Precision Green Sand Casting Ball Valve Parts]

Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Spped Weaving Machine 6]

Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China High Efficiency Automatic Glue Dispensing Machine with Factory Price, Part I.CNC Machining Mechanical Parts Fabrication Service. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Car Parking or Access Control 2.4 GHz Active RFID Card]

Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall.Electronic Pulse Gas Stove Burner Igniter Transformer Parts, 175-184[Factory best selling Captive Panel Screws - Stud bolt - Jiangyu ]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg]

Whitaker,J.R.(1963),CNC Turned Aluminum Anodized Auto Part. Analytical Chemistry35(12), 1950-1953[PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Line]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

SPA and Beauty Service Air Cleaning Ultrasonic Air DiffuserAuthor for correspondence