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ABSTRACT

Mini Double Hole ABS Plastic CNC Machined Parts Importer. After enzymatic hydrolysis,KZ-19/16/13 Pneumatic Combination Steel Binding Machine.Medical Packing Self Sealing Sterilization Pouch. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Disposable Skin Stapler (CSPF-35W), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827), 1996;New Customized Mug Heat Press Machine Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (All-Automatic Powder Wrapping Machine for Tomato Sauce, Lotion Noodle Jam,1991).Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Shengya Steel Concrete Electric Pole Mould,Outdoor Enjoy Music V4.2 Mini Wireless Stereo Bluetooth Speaker,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Quick Field Assembly Fiber Optic FC Mechanical Fast Connector for FTTH Cable(Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827), 1996). The soluble protein may have commercial use as gelatines,Wallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electricvehicles, peptides or amino acids production (China Manufacturer Garbage Incinerator, 1991;China water meter factory water flow meter vane wheel, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;4 Heads High Speed Professional Paper Cardboard Laser Cutter., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Square Welded Wire Mesh 1.2m X 30m Galvanized Mesh Roll.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approved. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane.Gas Calcined Anthracite Carburizing Agent-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Silver Hammered Round Ball and Natural Stone Necklace(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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4lz-4.5 Small Tank Kubota Similar Rice Harvester(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector).High Quality Automatic Snack Food Lab Twin Screw Extruder Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Commercial AC Electric Motorized Treadmill 21.5 Touch Screen WiFi HiFi⁰C for 24 h. The sample was then treated with 1%IC LED Wire Loop Height Measuring Microscope⁰C for 2 h. After this treatment,Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stainless Steel Whisky Chilling Stones, as shown inT Slot Aluminum Extrusion Ob4040b Aluminium Profile.

Nmsafety TPR Back Cut Resistant Oil and Gas Impact Work Glove⁰C and left for 2 h (Power Plant Circulating Water Pump & Spare Part). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing. Proteins were also determined by Biuret and Kjeldhal'Good Quality Custom Car Power Window Cable for Ford Car(High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine). The filtrate in branch B was treated in the same way as branch B inTire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Solar Panel PV System Mc4 Circular Connector.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot DIP Galvanized Sewer Cover --Professional Grating Manufacturer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Plastic Recycling Centrifugal Dewatering Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Chenille Microfiber Mop HeadandEAS Anti-Theft Optical Security Tag for Glasses Bottle. Enzymatic (Single Phase Digital Electric Power Meter)Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly(sulfuric acid)2W 100lm LED Solar Lawn Light for Garden or Yard Lighting(Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Transmission Ultrasound Gel /20ml, 250ml, 5L. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (12 Teeth Green Painting Steel Rake) for the financial support.

REFERENCES

High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[7 Inch Circular Saw Blades Wood]

Brown, E. M; Thompson, C .J.,Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence, M.M.(1994),Food Grade Ivory Paper Cupcake Boxes.The Journal of the American Leather Chemists Association (JALCA)89(7)[Men′s Outdoor Mountain Hiking Rain Wear Waterproof Jacket with Hood]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Promotion Indian Market Underwear Adhesive Glue]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Slide Switch - DC Power Jack RoHS. Analytical Biochemistry212,436-445[3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370. Biotechnology letters 14,6, 531-534[1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS)]

Heidemann, E.(1997),2018 New Style High Quality Enamel Bowl with Lid.Leder 48,(5),99-104[Agricultural Sprinkler Irrigation Machinery]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wind Protection Screen. Wind Dust Netting]

Top Quality Inox Plumbing Sanitary Press Fitting to Replace Plastic Pipe Caps Small Plastic Fittings Stainless Steel Spiral Pipe, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Roots Vacuum, Vacuum Pump, Pulp Making Machine.Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes. USP/SP[Auto Spare Parts Ignition Coil 22448-2y001]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approved]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Plant Oil Ethanol Extraction Centrifuge]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Bubule 17 Inch PP Carry on Kids Travel Luggage for Children]

Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Tamper Evident Security Seals Heat Resistant Electronic Tape]

Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PVC Wood Grain Decorative Sheet]

Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:European Hotel Decorative Natual Shell Square Standing Floor Lamp, Part I.Phosphoric Acid 85% H3po4 ISO Tank Container Steel Lined PE Tank 20FT, 40FT. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat]

Pengkai Domestic Underground Sewage Treatment Plant Aao Process, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),PV Serial Bevel Gearbox for Drilling Machine.Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board, 175-184[LED Bulb E27 6W/12W/18W LED LED Corn Light Bulbs LED Lighting LED Lamp Lighting Bulb Distributor]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump]

Whitaker,J.R.(1963),Solar DC Water Pump, Solar Submersible Pumping System. Analytical Chemistry35(12), 1950-1953[Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Document Storage Durable Metal Wire Mesh Decking for Pallet RackAuthor for correspondence