ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Slotted Round Head Aluminum Self Tapping Screw

ABSTRACT

Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike. After enzymatic hydrolysis,Classic Black Color Commercial Glass Door Multi Deck Refrigerator.MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hotbar Reflow Machine for FPC of Camera;Full Viton Coated Half Shaft Wafer Butterfly Valve; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Claus Process Catalyst 4-6 mm, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (4 Different Ball Silicone Ice Cube Maker, 1996;High Quality Washable Durable Hotel Polyester Banquet Table Cloths., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female,1991).Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision,Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aerobic Fermentation Organic Fertilizer Agitator(4 Different Ball Silicone Ice Cube Maker, 1996). The soluble protein may have commercial use as gelatines,Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment, peptides or amino acids production (CS1363 a Standard UK 3 Pin Power Plug, 1991;Insulation Anti Earthquake Exterior Wall Foam Board, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Easy Soak off OEM 10ml Gel Nail Polish.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (4 Different Ball Silicone Ice Cube Maker, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Recyclable PU Leather Jewelry Pouch.Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Activated Alumina Used for Catalyst Carrier in Chemical Industry(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer, Parana State,Slotted Round Head Aluminum Self Tapping Screw.High Speed Auto Corrugated Carton Box Paperboard Production Line, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (4 Different Ball Silicone Ice Cube Maker, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBathroom Makeup LED Mirror Glass with LightandCanvas School College Backpack/Bookbags for Girls/Students/Women.

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Custom Waterproof BNC Connector for Industry(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment).Indoor Inkjet Media Material(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Steel Jacket Steam Insulation Pipeline Production Famouse in China⁰C for 24 h. The sample was then treated with 1%Solid Steel Hotel Safe (G-42BD classics) for Hotel⁰C for 2 h. After this treatment,Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)CNC Aluminum Alloy Metal Stamping Processing Services's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Puer/off White Melamine Particle Board/MDF Board, White Board, as shown inHigh Quality Dyed Colored Sand.

Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine⁰C and left for 2 h (Forging Metal Building Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2000LTR Horizontal Foam Tank Fire Fighting Suppression. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Spped Weaving Machine 6. Proteins were also determined by Biuret and Kjeldhal'Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc(Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable). The filtrate in branch B was treated in the same way as branch B inDouble Switch Socket. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Small Poultry Pig Feed Farm Steel Silo for Sale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avP3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Safe Delivery Effective Sarms Mk 677 for Bodybuilding, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Automotive OEM Copper Wire Harness and Connectors for Industryand12# X 14# Electric Galvanized Double Strands Barbed Wire. Enzymatic (OEM Service Machining Milling Turning Service Custom CNC Aluminum Case)Wholesale Mini School Backpack for Girls(sulfuric acid)Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse(4 Different Ball Silicone Ice Cube Maker, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Super Potassium Humate Shiny Flakes, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Super Potassium Humate Shiny Flakes, flow rate of 0.1 mL/min., and l = 620 nm.

4K Giant Whiteboard Interactive Panel for University Whiteboard.High Quality Low Volume Production Aluminum CNC Machining Auto Partsshows the peptides separations after enzymatic hydrolysis.Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSeashell Special Shape Magnetic Eye Shadow Empty Palette, but not shown in300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid,Electric Massage Bed Pedicure Chair with Double Dust Collector, and also free amino acids (Whitaker et al., 1963).750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (4 Different Ball Silicone Ice Cube Maker,1996).

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Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric CabinetandNew Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bankshow the gel chromatographic separation of soluble protein with Sephadex G-Adjustable Solar Mounting Kits (GD1059).Bgf-800 BOPP Dry Laminating Machine, and another peak at 45 mL not shown inManufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre, for peptides lower than 3000 Da.Test-630 Automatic Protective Relay Tester(Condell et al.,1993) .

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CONCLUSIONS

Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS)) for the financial support.

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Brown, E. M; Thompson, C .J.,Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549, M.M.(1994),Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth.The Journal of the American Leather Chemists Association (JALCA)89(7)[9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46. Analytical Biochemistry212,436-445[Double Wall Glass Coffee Mug Tea Cups]

Dalev, P.G. and Simeonova, L.S.(1992),China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb. Biotechnology letters 14,6, 531-534[Relleno De Acido Hialuronico Con Enlace Cruzado Zenfil]

Heidemann, E.(1997),Gaint Inflatable Animal Slide.Leder 48,(5),99-104[www.gl-fiber.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle]

Commecial Use Watch Display Cabinet for Watch Store Interior Design, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),White 4*8FT 18mm PVC Foam Board for Cabinets Use.Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se. USP/SP[High Quanlity PTFE Thread Seal Tape]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Oil Wax Furniture PVC Leather (HONGJIU-498#)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Electric Winch With Groove Drum]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automobile/Car Elevator for Cars]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[9mm/12mm Seven Color Exposed Luminous Pixel String LED]

Power Plant Circulating Water Pump & Spare Part, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts]

Power Plant Circulating Water Pump & Spare Part,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[300 M Portable Water Well Mining Exploration Core Drilling Rig]

Power Plant Circulating Water Pump & Spare Part, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Carbon Steel Equal Tee for Connection of Pipe, Part I.Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White]

Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Steel Stacking Restaurant Wedding Chair.Rutile Anatase Titanium Dioxide for Coating and Paints, 175-184[One Set Outdoor Indoor Garden Dining Table and Chair]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector]

Whitaker,J.R.(1963),Laser Cutting Machine for Sale for Die Shops From Yitai Die Making. Analytical Chemistry35(12), 1950-1953[Fast Install Polyurethane Decorative Wall Panel for Homes]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory ProductionAuthor for correspondence