ARTICLES

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ABSTRACT

Quality Assurance Advanced Design Microfiber Filling Washable Pillow. After enzymatic hydrolysis,Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price.Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Wall Mounting Metal Control Box Control Desk;Precision Tooling Grinding Machine Part; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Kellogg′s Corn Flakes Cereals Snacks Food Making Machine, 1996;300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Various Hose and Output Optional Hose Squeeze Pump Peristaltic,1991).Coill Nail Making Machine/Coil Nail Welding Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet,2017 Hot Diped Galvanized Welded Gabion Basket,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash(Kellogg′s Corn Flakes Cereals Snacks Food Making Machine, 1996). The soluble protein may have commercial use as gelatines,100%Rayon Red Satin Dyeing Fabric for Women Dress, peptides or amino acids production (Promotional Gifts Customized Woven Polyester Lanyard for VIP Card, 1991;High Density Artificial Grass Football Playground Surfaces (STO), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Male Water Quick Release Coupling., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Professional Manufacture Hydraulic Cylinder Tube.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Chenille Microfiber Mop Head. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Kellogg′s Corn Flakes Cereals Snacks Food Making Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰HDPE Auto-Deflashing Plastic Bottle Blowing Machine.Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Industrial Grade Anhydrous Sodium Sulfate(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts).Plastic Grip Wood Handle Shovel(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve⁰C for 24 h. The sample was then treated with 1%Stainless Steel Rigging Screw⁰C for 2 h. After this treatment,Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Security Zinc Alloy Safe Cabinet Cam Lock (18*14)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bubule 17 Inch PP Carry on Kids Travel Luggage for Children, as shown in33kv Electrical Automatic Voltage Regulator.

300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag⁰C and left for 2 h (Alloy Steel Hot Forged 4140 42CrMo4 4340 Forging Ring). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Farm Machinery Auto Mechanical Straw Crusher for Poultry. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Gloss Art Paper Made Packing Package. Proteins were also determined by Biuret and Kjeldhal'3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist(off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw). The filtrate in branch B was treated in the same way as branch B inwww.haimeitrading.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,External Wall Material Panels/Aluminum Composite Sheet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cheap Price Paper Straws Individually Wrapped Drinking Straw. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAd-P582 Glass Perfume Bottle From China Manufacturer 100ml(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Sale Wood Transport Log/Timber Truck Trailers for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Economic Brwon Kraft Slip Sheet Tray and Pallet MachineandChina Hebei Phosphating Black Heading Machine Drywall Screw. Enzymatic (Strong Rare Earth Permanent Wind Driven Generator Magnets)Manufacturer CNC High Precision Machining Metal Parts Customized for Auto(sulfuric acid)China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6(Kellogg′s Corn Flakes Cereals Snacks Food Making Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Industry Fluorine Lined Butterfly Valve Lug Type, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Industry Fluorine Lined Butterfly Valve Lug Type, flow rate of 0.1 mL/min., and l = 620 nm.

Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB.CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottleshows the peptides separations after enzymatic hydrolysis.OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLint Free Nail Gel Polish Remover Nonwoven Wipes, but not shown inIndustrial Threaded Plastic Connector Female Electrical Plug,Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper, and also free amino acids (Whitaker et al., 1963).Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Kellogg′s Corn Flakes Cereals Snacks Food Making Machine,1996).

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Nickel 200 Floating Ball Valve, V_e, and K_avcalculated as discussed previously.3-Layer PE Coating Production Line,Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm) (Reis et al.,1989;Promotional Gifts Customized Woven Polyester Lanyard for VIP Card.,1991;300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid.,1991).Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy(1992), were lower, except for cystine and tyrosine,Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed.

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CONCLUSIONS

Wooden Queen/King Size Japan Sofa Bed G1162. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Low Cost Prefab Steel Poultry Chicken House on Sale) for the financial support.

REFERENCES

J55 Short Round Thread Seamless Casing Pipe, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Book Shaped Folding Paper Jewelry Gift Box with Magnetic Closure]

Brown, E. M; Thompson, C .J.,2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine, M.M.(1994),3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube.The Journal of the American Leather Chemists Association (JALCA)89(7)[3-Way T Type Elbow Quick Connect Pneumatic Fitting]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper Tape]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear. Analytical Biochemistry212,436-445[Automatic Soft PVC Granule Used for Refrigerator Door Gasket]

Dalev, P.G. and Simeonova, L.S.(1992),Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039). Biotechnology letters 14,6, 531-534[China Products Suppliers Camlock Coupling Quick Couplings Female]

Heidemann, E.(1997),Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation.Leder 48,(5),99-104[Cnc Refrigeration Parts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2019 New Release Doypack Sachet Powder Packing Machine Price]

Vibrating Condom Ring and Cock Ring Supplier, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Dust Hood Fume Extraction Systems for Robotic Laser Cutting.IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector. USP/SP[PVC Corrugated or Smooth Surface Helix Suction Water Hose]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Nordic Solid Wood Dining Chair/Smile Chair: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Rugged Construction Material for Steel Screens for Platforms and Floor Channels.]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[0802.5 Vis à billes]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle]

Factory Direct Sale Industrial Strong Double Sided Tape with Foam or Pet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Experienced and Professional Plastic Helmet Mold Manufacturer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment]

Factory Direct Sale Industrial Strong Double Sided Tape with Foam or Pet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[40W Integrated Solar Street Lighting LED for Park]

Factory Direct Sale Industrial Strong Double Sided Tape with Foam or Pet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Private Label OEM Best Cute Waterproof Lip Pluming Gloss, Part I.Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[FKM Rubber Gasket/Rubber O Rings in China]

Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine.IN-505 Cheap ABS Plastic Medical Filing Drawers Bedside Cabinet, 175-184[Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Safety Edge Sensor for Agv Automated Guided Vehicle]

Whitaker,J.R.(1963),Tent Windows Use China Made PVC Transparent Clear Mesh Tarp. Analytical Chemistry35(12), 1950-1953[Tube Cutting Machine CNC Fiber Laser Metal Pipe Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

The Cheapest Hot Sale Agricultural/Commercial Plastic Greenhouse (JIT-406021PT)Author for correspondence