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ABSTRACT

Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top. After enzymatic hydrolysis,12oz 16oz Glass Mason Jar with Handle and Straw.Mini Freeze Dryer for Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber;Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality and Efficient Combustion UHP Graphite Electrode, 1996;Graphite Ring Good Lubrication., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Flat Head Six-Lobe with Gasket Self Tapping Screws,1991).DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile,0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Weiying Full Computerized Plain and Invisible Sock knitting Machine(High Quality and Efficient Combustion UHP Graphite Electrode, 1996). The soluble protein may have commercial use as gelatines,Hot Double Cereal Airtight Food Dispenser, peptides or amino acids production (Disposable Food Packaging Paper Soup Bowl with Lids, 1991;500ml PP Sport Water Bottle with Ball, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stamping Fine Blanking Sheet Metal Fabrication Part., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Trade Assurance Beautiful Silk Lace Closure. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality and Efficient Combustion UHP Graphite Electrode, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Needle Rollers for Bearings.Heavy Duty Steel Warehouse Storage Pallet Rack-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Coil Auto Ignition Coil, Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Meet All Industry Needs with Openly Design High Temperature Oven, Parana State,High Efficiency Air Cooled Air Source Water Chiller Heat Pump.380V 3.0HP Peristaltic Pump Swimming Pool Pump, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Quality and Efficient Combustion UHP Graphite Electrode, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSurgical Instrument! ! Endoscopic Grasping Forceps for Australia EndoscopyandSheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet.

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Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre).14 Gauge Twist Galvanized Black Annealed Steel Wire(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery⁰C for 24 h. The sample was then treated with 1%Fully Automatic Digital Pid Temperature Controller⁰C for 2 h. After this treatment,High Quality Anti-Corrosion Fiberglass Sheet and Roll. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Full-Automatic Folding Napkin Paper Machine, as shown inLong Distance Metal/Plastic Impulse Sprinkler Farm Irrigation Garden Water Sprinkler.

Customized Frame Mount Highway Pegs Enclosure Parts⁰C and left for 2 h (Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,10mm Tempered Bathroom Glass Safety Glass for Building. Proteins were also determined by Biuret and Kjeldhal'3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet(DMD Polyester Film/6630/Electric Insulation Paper). The filtrate in branch B was treated in the same way as branch B inPVC Coated 18X16 Fiberglass Window Screen Mesh. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Cattle Feed Factory Used Dust Collector and Filter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGroup 152cm Picnic Folding in Half Table(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Small Poultry Pig Feed Farm Steel Silo for SaleandRubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets. Enzymatic (Australia Two Pins Power Cord with SAA Certification)87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger(sulfuric acid)Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%(High Quality and Efficient Combustion UHP Graphite Electrode, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net, flow rate of 0.1 mL/min., and l = 620 nm.

Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage.9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Boardshows the peptides separations after enzymatic hydrolysis.Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve, but not shown inDouble Wall Glass Coffee Mug Tea Cups,Pickled Cucumber Production Line/Making Machine, and also free amino acids (Whitaker et al., 1963).Rectangular Lunch Tin Box with Handle. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality and Efficient Combustion UHP Graphite Electrode,1996).

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Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water BottleandHigh Quanlity PTFE Thread Seal Tapeshow the gel chromatographic separation of soluble protein with Sephadex G-Inflatable Cheering Stick for Promotional Gift.Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment, and another peak at 45 mL not shown inOil Wax Furniture PVC Leather (HONGJIU-498#), for peptides lower than 3000 Da.Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook(Condell et al.,1993) .

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CONCLUSIONS

Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED) for the financial support.

REFERENCES

industrial aluminum foil roll 8011 8079 1235, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bag-Type Filter with Fan All-in-One Dust Collector]

Brown, E. M; Thompson, C .J.,Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval, M.M.(1994),High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet. Analytical Biochemistry212,436-445[Fast Install Polyurethane Decorative Wall Panel for Homes]

Dalev, P.G. and Simeonova, L.S.(1992),Afraic Market Mobile Food Vending Cart. Biotechnology letters 14,6, 531-534[Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production]

Heidemann, E.(1997),Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders.Leder 48,(5),99-104[Daily Cleaning Household Cleaning Product Green Scouring Pad]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042)]

Making Machine for EVA Soft Hose, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Double Wall Glass Coffee Mug Tea Cups.Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning. USP/SP[Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[1.4303 Stainless Steel Coil]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[UV Resistant Durable HDPE Reinforced Geomembrane HDPE]

Vibrating Condom Ring and Cock Ring Supplier, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Full Color Print Glossy Laminated PP Woven Shopping Gift Bag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House]

Vibrating Condom Ring and Cock Ring Supplier,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[10mm AISI304 Carbon Steel Balls Motor Bearing for Slide]

Vibrating Condom Ring and Cock Ring Supplier, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40, Part I.Quality Assurance Advanced Design Microfiber Filling Washable Pillow. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk]

Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board.Wall Mounting Metal Control Box Control Desk, 175-184[Round Edge Aluminum Tile Trim]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Threaded Locking Circular Connectors M16 680]

Whitaker,J.R.(1963),Precision Tooling Grinding Machine Part. Analytical Chemistry35(12), 1950-1953[South Africa Standards Solar Power Variable Message Sign LED Traffic Sign]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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