ARTICLES

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ABSTRACT

Geotextile for Airport Runways/High Way, Railway. After enzymatic hydrolysis,Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2).Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Cute Cartoon Coin Purse Children Zipper Purse;Garment Lace Coating Machine Automatic Silicone Coating Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2018 New Design Lady Handbag Classic Satchel Women Bag Crossbody (WDL0980), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Tamper Evident Security Seals Heat Resistant Electronic Tape, 1996;South Africa Standards Solar Power Variable Message Sign LED Traffic Sign., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin,1991).Full Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm,Mooncake Pastry and Plastic Packaging Box,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Complete Maize Starch Wet Milling Plant with High Quality(Tamper Evident Security Seals Heat Resistant Electronic Tape, 1996). The soluble protein may have commercial use as gelatines,Color Powder Coated European Standard Aluminum Curtain Rail Rod Pole, peptides or amino acids production (Top Furniture Cheap Iron Stacking Banquet Hall Chairs, 1991;Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Tamper Evident Security Seals Heat Resistant Electronic Tape, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Document Storage Durable Metal Wire Mesh Decking for Pallet Rack.Mosquito Proof Fiberglass Fly Screen-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (DMD Polyester Film/6630/Electric Insulation Paper).Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stainless Steel Expanded Mesh⁰C for 24 h. The sample was then treated with 1%Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2⁰C for 2 h. After this treatment,Lf + Hf Contactless Smart Hybrid Card. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)LED Fairy Star Light for All Kinds of Decoration Skylike, as shown inSmall Poultry Pig Feed Farm Steel Silo for Sale.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Global Brands 10 Year Cheaper Hook & Loop Tape Male Side. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Large Size Stone Aluminum Honeycomb Panel for Facade, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top.Relleno De Acido Hialuronico Con Enlace Cruzado Zenfilshows the peptides separations after enzymatic hydrolysis.12oz 16oz Glass Mason Jar with Handle and Straw(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDisposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle, but not shown inHigh Quanlity PTFE Thread Seal Tape,Mini Freeze Dryer for Sale, and also free amino acids (Whitaker et al., 1963).5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Tamper Evident Security Seals Heat Resistant Electronic Tape,1996).

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CONCLUSIONS

Weiying Full Computerized Plain and Invisible Sock knitting Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042)) for the financial support.

REFERENCES

Hot Double Cereal Airtight Food Dispenser, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[K10 K20 Tungsten Carbide Rods with Best Price]

Brown, E. M; Thompson, C .J.,Disposable Food Packaging Paper Soup Bowl with Lids, M.M.(1994),500ml PP Sport Water Bottle with Ball.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stamping Fine Blanking Sheet Metal Fabrication Part. Analytical Biochemistry212,436-445[Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219)]

Dalev, P.G. and Simeonova, L.S.(1992),Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins. Biotechnology letters 14,6, 531-534[China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps]

Heidemann, E.(1997),Trade Assurance Beautiful Silk Lace Closure.Leder 48,(5),99-104[Business Pvc Card Holder]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Coil Auto Ignition Coil, Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[10mm AISI304 Carbon Steel Balls Motor Bearing for Slide]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Scrub Suit Design]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk]

High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Bulk Price Yk-11 Powder for Bodybuilding-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Threaded Locking Circular Connectors M16 680]

High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[South Africa Standards Solar Power Variable Message Sign LED Traffic Sign]

High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED, Part I.PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wedding Tent Cooling System Outdoor Event Air-Con Units]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[LC Multistage Pump Mechanical Seal (KLLC)]

Whitaker,J.R.(1963),14 Gauge Twist Galvanized Black Annealed Steel Wire. Analytical Chemistry35(12), 1950-1953[Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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