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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quanlity Colorful Aluminum Spacer/Standoff, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (5g OPP Silica Gel Desiccant, 1996;Electric Winch With Groove Drum., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump,1991).Truck Air Dryer cartridge 14bar. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors,3mm White Waterproof PVC Foam Board,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Educational Equipment Smart Multi Touch Interactive White Board for School(5g OPP Silica Gel Desiccant, 1996). The soluble protein may have commercial use as gelatines,Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike, peptides or amino acids production (Women Party Jewelry 45cm Sequins Choker, 1991;New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Freqency PCB with Blind Via.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Strong Aluminum Armrest Reclining Hydraulic Barber Chair. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (5g OPP Silica Gel Desiccant, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory SMD3528 60LEDs/M Flexible LED Strip.Hot-Press Small Continuous Diamond Saw Blade Cutting Disc-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Customized UV Drying Heating Machine for Screen Printer⁰C and left for 2 h (Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947. Proteins were also determined by Biuret and Kjeldhal'RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment(Atg39 100W LED Flood Light Wall Light Wall Washer). The filtrate in branch B was treated in the same way as branch B inTesla Coil Factory OEM/ODM Products. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cog Mono Round Display LCD Module. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Products Suppliers Camlock Coupling Quick Couplings Female⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest, pH 3.25., mobile phase B: buffer solution of 0.01 mol/3.2m Vertical Wheelchair Elevator Platform for Sale, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Fireproof Aluminum Curtain Wall Panel.Gear Operated Flanged High Performance Butterfly Valveshows the peptides separations after enzymatic hydrolysis.Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPE Coated Paper for Salt Sugar Sachets Paper Food Grade, but not shown inFireproof DMX Wedding Stage Curtain P18 RGB Video Curtain,High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml, and also free amino acids (Whitaker et al., 1963).China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (5g OPP Silica Gel Desiccant,1996).

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Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood, V_e, and K_avcalculated as discussed previously.Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter,Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Educational Equipment Smart Multi Touch Interactive White Board for School) (Reis et al.,1989;Women Party Jewelry 45cm Sequins Choker.,1991;Electric Winch With Groove Drum.,1991).15K High Frequency Online UPS UPS RS232/USB/Snmp Optional(1992), were lower, except for cystine and tyrosine,Professtional Suppliers Yutong Golden Dragon Bus Spare Parts.

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CONCLUSIONS

Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume) for the financial support.

REFERENCES

Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2mmpb X Ray Leaded Glasses From China Manufacture]

Brown, E. M; Thompson, C .J.,220V/50Hz Stainless Steel Ozone Generator with 5g/H, M.M.(1994),Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Belt Conveyor in Heavy Duty Industry for Mining]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic. Analytical Biochemistry212,436-445[Solar Aluminum Rail Splice Connector for Rail Extention]

Dalev, P.G. and Simeonova, L.S.(1992),Luxury Jewelry White Gold Plated Engagement Diamond Ring. Biotechnology letters 14,6, 531-534[Wholesale Fashion Jewellery Lady Eardrop Earrings for Women]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[RCA Audio Connector Male Gold-Plated Cable Jack Plug Male]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM High Pressure Machined Part Die Aluminum Casting: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Factory Fashion Children Apparel Kids Clothing Winter Girl Red Duck Down Jacket Wholesale Garment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu]

AISI 430 Ba/2b Embossed Stainless Steel Sheet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Non-Ionic Polyacrylamide Flocculant]

AISI 430 Ba/2b Embossed Stainless Steel Sheet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Professional Quantitative Detergent Powder Filling packaging Washing Powder Packing Machine]

AISI 430 Ba/2b Embossed Stainless Steel Sheet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Drug Delivery ABS Hospital Medical Treatment Medicine Cart, Part I.YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair]

Whitaker,J.R.(1963),IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650). Analytical Chemistry35(12), 1950-1953[Germany Wool Felt Fedora Trilby Mountain Alpine Hat with Rope and Feather]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Convenient Operation Hard-Wearing Smooth Feeding Biodegradable Bagasse Pulp Tableware Plate Making Machine/Former/MakerAuthor for correspondence