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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Economy Combo Heat Press for Starter. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Long Distance Metal/Plastic Impulse Sprinkler Farm Irrigation Garden Water Sprinkler

ABSTRACT

Food Grade Beverage Clear PVC Tubing Hose. After enzymatic hydrolysis,Imee Printing Eco Friendly Promotional Drawstrings Craft Kraft Paper Cardboard Clothing Foldable Shopping Gift Bag.Wet and Wavy Remy Mink Brazilian Hair Weave Bundles Deep Wave. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Safety Shoes Anti-Slip Silicone Rubber Crampons OEM Factory, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (45ton Proof Load Testing Water Bag for Life Boat, 1996;Sheet Metal Parts Stamping Processing Cold Rolling Plate Cutting Stamping Bending Precision., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Double Coil Control Solenoid Valve of Butterfly Valve,1991).High Frequency Online UPS W/3W-20K. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Solar Collector Panel for Pool Heater,ASTM A500 Gr. a/B/C Steel Square Tubing Square Tubing Sizes From China Pipe Manufacturers,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aluminum Sheet Metal Case Parts for Industrial Computer(45ton Proof Load Testing Water Bag for Life Boat, 1996). The soluble protein may have commercial use as gelatines,Black Plastic Handle Stainless Steel Kitchen Gadget, Kitchen Tools Set, peptides or amino acids production (High Quality Hydraulic Cylinder Tubes for Hydraulic Cylinder, 1991;RCA Audio Connector Male Gold-Plated Cable Jack Plug Male, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Egg 0b 309 Fixed Panel Socket [Push Pull Circular Connector., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.A19 6W 10 Red & 4 Blue LED Plant Light LED Grow Light Bulb.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Premium quality for Potassium Humate powder. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (45ton Proof Load Testing Water Bag for Life Boat, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wedding Cake Knife Serving Set Stainless Steel Acrylic Handle.Home Design Solid Wood Double Adult Bed Bedroom Furniture-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Special Shape Spring with Good Quality and Fast Delivery(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Nordic Solid Wood Dining Chair/Smile Chair).Better Quality White Coated Fbb Paper Ivory Board(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Excellent Plastic Extruder in Pet PP Packing Strap Machine⁰C for 24 h. The sample was then treated with 1%Food Zip Lock Square Bottom Brown Paper Coffee Bag Supplier From China⁰C for 2 h. After this treatment,China Factory Supplying High Purity Magnesium Alloy Ingot 99.0%~99.9%. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2019 New Advertising Inflatable LED Jellyfish Balloon for Sale, as shown inModern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting.

Tree Root Grinder Stump Grinder⁰C and left for 2 h (High Efficiency Automatic Multihead Weigher Packing Machine for Raisin/Legume). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Open Narrow Slot Kx Electrical Wire Cable Raceway Ducts/Yellow Jacket Cable Protector. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Customization! Bdy380CD Horizontal Directional Drilling Machine. Proteins were also determined by Biuret and Kjeldhal'V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine(Test-630 Automatic Protective Relay Tester). The filtrate in branch B was treated in the same way as branch B inGranite Material. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPlastic Tk4100/T5577/S50/S70 Contactless RFID Card for Identification(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Professional Manufacturer Pure Wolfram Wire with High Purity 99.95%⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/FKM Bearing Oil Seal /Customized, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Selling Shandong Manufacturer 3 4 5 Inch Rubber Sealing Ring Joint UPVC Rrj Pipe for Water Supply and Sewage/Drainage 8kg Rate PVC Tubes, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Feed Storage OEM Grain Storage Silo 1cbm to 200cbm.Activated Alumina Used for Catalyst Carrier in Chemical Industryshows the peptides separations after enzymatic hydrolysis.Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGlossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink, but not shown inFully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers,Captain Woodwork Sanding Machine/Sanding Table, and also free amino acids (Whitaker et al., 1963).Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (45ton Proof Load Testing Water Bag for Life Boat,1996).

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CONCLUSIONS

Yet905 Wireless Keypad Doorbell Kits with Remote Control. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Boundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence) for the financial support.

REFERENCES

Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Factory Supply Non-Bituminous Self Adhesive Butyl Waterproof Tapes]

Brown, E. M; Thompson, C .J.,Original Sinotruk HOWO Truck Parts - Exhaust Pipe Wg9725542042, M.M.(1994),China Factory Queen Mattress with Visco Memory Foam and Latex.The Journal of the American Leather Chemists Association (JALCA)89(7)[2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Multi-Port Ss 304 Manual Three Way Non-Retention Ball Valve. Analytical Biochemistry212,436-445[Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Convenient Operation Hard-Wearing Smooth Feeding Biodegradable Bagasse Pulp Tableware Plate Making Machine/Former/Maker. Biotechnology letters 14,6, 531-534[Mini High Temperature Clothing Labels Screen Printing Machine Factory]

Heidemann, E.(1997),HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance.Leder 48,(5),99-104[Welded And Seamless Stainless Steel Flange]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Metal Cutting Machine CNC Plasma Cutter]

Hot Water Pipe Insulation Steam, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Ceiling Mounted IV Stand Stainlesss Steel I V Pole.Pipe Connection Types of Pressing Scaffolding Universal Clamp. USP/SP[Workbench Anti-Static Table ESD Natural Rubber Table Mat]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Adjustable Solar Mounting Kits (GD1059)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ml33-2mm Eddy Current Linear Position/Displacement Sensor: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Security Zinc Alloy Safe Cabinet Cam Lock (18*14)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Caster Axle]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Forged 316 Stainless Steel Marine Twisted Long D Shackle]

Veterinarian Ultrasound Diagnostic System for Cow, Horse, Swine, Goat, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[22.8V 150W Medical Halogen Lamp]

Veterinarian Ultrasound Diagnostic System for Cow, Horse, Swine, Goat,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies]

Veterinarian Ultrasound Diagnostic System for Cow, Horse, Swine, Goat, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale Long Lasting Custom Private Label Glitter Lip Gloss, Part I.ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Various Hose and Output Optional Hose Squeeze Pump Peristaltic]

10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School.High Quality Dyed Colored Sand, Natural Coloured Sand, 175-184[Wood Chipper Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip]

Whitaker,J.R.(1963),China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps. Analytical Chemistry35(12), 1950-1953[Gaint Inflatable Animal Slide]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Lamella Clarifier for Wastewater Treatment PlantAuthor for correspondence