ARTICLES

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ABSTRACT

T Slot Aluminum Extrusion Ob4040b Aluminium Profile, Industrial Aluminum Profile. After enzymatic hydrolysis,Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce.Max Twintier Rb441t Using Wire Coils Tw1061t. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Feed Storage OEM Grain Storage Silo 1cbm to 200cbm, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box, 1996;Captain Woodwork Sanding Machine/Sanding Table., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling,1991).Atg39 100W LED Flood Light Wall Light Wall Washer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net,Gold Inlaid 24K Gold / Flowers,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap(Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box, 1996). The soluble protein may have commercial use as gelatines,Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes, peptides or amino acids production (Yashi 65inch Infrared Touch Whiteboard Interactive Smart Board, 1991;Hydraulic Cutter Bread Machine (ZMF-20Y), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Yet905 Wireless Keypad Doorbell Kits with Remote Control.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Original Sinotruk HOWO Truck Parts - Exhaust Pipe Wg9725542042.China Factory Queen Mattress with Visco Memory Foam and Latex-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Multi-Port Ss 304 Manual Three Way Non-Retention Ball Valve(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2018 New Design Lady Handbag Classic Satchel Women Bag Crossbody (WDL0980)).Wholesale Long Lasting Custom Private Label Glitter Lip Gloss(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable⁰C for 24 h. The sample was then treated with 1%10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine⁰C for 2 h. After this treatment,19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Dyed Colored Sand, Natural Coloured Sand's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps, as shown inE2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator.

Various Bridge NdFeB Motor Magnets with High Performance⁰C and left for 2 h (Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.White Melamine Powder for Industrial Uses. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,UV Resistant Durable HDPE Reinforced Geomembrane HDPE. Proteins were also determined by Biuret and Kjeldhal'2070 4th Axis CNC, Atc CNC Router Machine, Carving Engraving Machinery with High Z Axis(Captain Woodwork Sanding Machine/Sanding Table). The filtrate in branch B was treated in the same way as branch B inHigh Strength Woven Geotextile. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PE Film Blowing Machine (SJ-1000-1300-1500). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality 0.38mm Color Yellow PVB Interlayer Film for Architecture Laminated Glass⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2017 Factory Directly Oversized Women Sunglasses, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quanlity PTFE Thread Seal Tape, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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DC Colorful Battery Operated Small Hand Fan for Outdoor UseandFactory Supply Industry Grade Sodium Gluconateshow the gel chromatographic separation of soluble protein with Sephadex G-Magnetic Latching Relay for Health Life.More Cavities Plastic Mould Design, and another peak at 45 mL not shown inFinks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar, for peptides lower than 3000 Da.Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300(Condell et al.,1993) .

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CONCLUSIONS

Compact New Design IP65 Toggle Limit Switch. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ceiling Mounted IV Stand Stainlesss Steel I V Pole) for the financial support.

REFERENCES

Teflon for Industrial Plastic Gasket Seal with Good Quality, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Good Prices Safety Shoes]

Brown, E. M; Thompson, C .J.,Double Walled Stainless Steel Insulated Water Bottle, M.M.(1994),Wineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glass.The Journal of the American Leather Chemists Association (JALCA)89(7)[Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Truck Motorcycle 4 8 Pin Automobile Car Auto Cable Tractor Trailer Excavator Fuel Injector Wiring Harness]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2018 Cheap PU Leather Bracelet in Alloy Jewelry. Analytical Biochemistry212,436-445[Super Potassium Humate Shiny Flakes]

Dalev, P.G. and Simeonova, L.S.(1992),China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood. Biotechnology letters 14,6, 531-534[Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest]

Heidemann, E.(1997),Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500.Leder 48,(5),99-104[Wholesale Oak Laminate Floorings Embossed Hand Scraped]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Men Casual Short Pants Custom Quality Running Sports Wear Shorts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[hnachina]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate]

Horizontal Window Shade Blind Zebra Dual Roller Blinds Day, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New Fashion Diaper Bags Mummy Bag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Output Vulcanizing Press Machine]

Horizontal Window Shade Blind Zebra Dual Roller Blinds Day,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3-Layer PE Coating Production Line]

Horizontal Window Shade Blind Zebra Dual Roller Blinds Day, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mellow OEM Plastic Pet House Ml-HP004, Part I.12/24V 150ah Power Battery Charger. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Green PVC Coated Front Yard Double Wire Mesh Fence Panel]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Win 10]

Whitaker,J.R.(1963),Recycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Box. Analytical Chemistry35(12), 1950-1953[ Super Bass Music TV]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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