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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Drug Delivery ABS Hospital Medical Treatment Medicine Cart. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black

ABSTRACT

Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress. After enzymatic hydrolysis,Colorful Electric English Funny Music Story Module Printing Book.2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650);Tilt-and-Turn-with-Hidden-Hinge; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Automatic Soft PVC Granule Used for Refrigerator Door Gasket, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (0.45um Dia 47mm PVDF Filter Membrane, 1996;Commecial Use Watch Display Cabinet for Watch Store Interior Design., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring,1991).Slotted Round Head Aluminum Self Tapping Screw. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike,Classic Black Color Commercial Glass Door Multi Deck Refrigerator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker(0.45um Dia 47mm PVDF Filter Membrane, 1996). The soluble protein may have commercial use as gelatines,Hotbar Reflow Machine for FPC of Camera, peptides or amino acids production (Full Viton Coated Half Shaft Wafer Butterfly Valve, 1991;Claus Process Catalyst 4-6 mm, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;4 Different Ball Silicone Ice Cube Maker., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Washable Durable Hotel Polyester Banquet Table Cloths.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (0.45um Dia 47mm PVDF Filter Membrane, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe.200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box).Recyclable PU Leather Jewelry Pouch(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11)⁰C for 24 h. The sample was then treated with 1%Activated Alumina Used for Catalyst Carrier in Chemical Industry⁰C for 2 h. After this treatment,High Quality 45W 50W Commercial Recessed COB LED Downlight. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS), as shown inPneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Steel Jacket Steam Insulation Pipeline Production Famouse in China. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSolid Steel Hotel Safe (G-42BD classics) for Hotel(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CNC Aluminum Alloy Metal Stamping Processing Services⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Puer/off White Melamine Particle Board/MDF Board, White Board, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting.High Quality Bucket Segment Bolt 6V0937shows the peptides separations after enzymatic hydrolysis.Made in China 12 Inch Aluminum Flexible Duct Pipe(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in2015 New Square Folding Ajustable Wall LED Bathroom Mirror, but not shown inRefillable Airless Cosmetic Packaging 10ml 15ml 20ml 30ml Serum Bottle,18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring, and also free amino acids (Whitaker et al., 1963).Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (0.45um Dia 47mm PVDF Filter Membrane,1996).

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Wholesale Mini School Backpack for Girls, V_e, and K_avcalculated as discussed previously.Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse,Super Potassium Humate Shiny Flakes. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module) (Reis et al.,1989;Full Viton Coated Half Shaft Wafer Butterfly Valve.,1991;Commecial Use Watch Display Cabinet for Watch Store Interior Design.,1991).4K Giant Whiteboard Interactive Panel for University Whiteboard(1992), were lower, except for cystine and tyrosine,Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25.

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CONCLUSIONS

Electric Massage Bed Pedicure Chair with Double Dust Collector. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CS1363 a Standard UK 3 Pin Power Plug) for the financial support.

REFERENCES

750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Abarelix Acetate Gmp Exporter]

Brown, E. M; Thompson, C .J.,Adjustable Solar Mounting Kits (GD1059), M.M.(1994),Bgf-800 BOPP Dry Laminating Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Lotion Noodle Jam]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Test-630 Automatic Protective Relay Tester. Analytical Biochemistry212,436-445[Flat Roof Insulation Board Heat Insulation Board]

Dalev, P.G. and Simeonova, L.S.(1992),Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle. Biotechnology letters 14,6, 531-534[125mm Wet Diamond Pad for Polishing Marble]

Heidemann, E.(1997),Tpdl Belt Press Sludge Equipment with Slaughtering Machine.Leder 48,(5),99-104[36W Long Distance LED Flood Light for Sports Field with Factory Price]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Di003 Environmental Protection Against The Stench Floor Drain]

High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS).The Most Demanded Products Fluorescence Microscope Camera. USP/SP[LSR Injection Mold Tooling for Medical Nipple]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[5km City Surveillance Network Infrared Laser PTZ Camera]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Low Price Promotion Soda Ash Light to Papermaking: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[3l Composite Material Scuba Tank]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Current Precise LED Digital Ammeter]

Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),HOWO 420HP Rhd Tractor Truck Head-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automatic Controller Carbon Steel Sheet Pipe China CNC Plasma Cutter]

Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Equipment Manufacturers Mayflay Sales Semi-Auto Dry - Suction Commercial Sandblaster for Motorcycle Frame Surface Cleaning with Light Gun & Nozzle]

Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549, Part I.Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3]

Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb.Gaint Inflatable Animal Slide, 175-184[m.xzmhglassware.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sport Anti Vibration Hiking Safety Shoes]

Whitaker,J.R.(1963),White 4*8FT 18mm PVC Foam Board for Cabinets Use. Analytical Chemistry35(12), 1950-1953[Commercial AC Electric Motorized Treadmill 21.5 Touch Screen WiFi HiFi]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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