ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Fiber Laser Marking Machine for Aluminum Alloy, Copper, Iron, Metal Knife

ABSTRACT

Refillable Airless Cosmetic Packaging 10ml 15ml 20ml 30ml Serum Bottle. After enzymatic hydrolysis,Customized Thickness EVA High Density Foam EVA Roll.Low Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Carbide Mould Parts Moulding Parts;Compression Mens Customized Gym Shorts Sportswear; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder, 1996;Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display,1991).Electric Furniture Lift. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter,Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Construction Material Hoist Rack and Pinion(99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder, 1996). The soluble protein may have commercial use as gelatines,3000lb Small Boat Marine Electric Trailer Winch Power-in, Power-out, peptides or amino acids production (Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks, 1991;Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2018 Classic New Design Metal Optical Glasses Men and Women Reading Eyeglasses., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Endoscopic Surgical Adult Disposable Bite Block.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China OEM ABS Clear Plastic Injection Molding. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Widely Use 32 Cavities Pet Preform Water Bottle Mould.Black Powder Coated Steel Sheet Metal Housing-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Alloy Steel Hot Forged 4140 42CrMo4 4340 Forging Ring(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Heavy Caliber Copper Rectangular Steel Tube, Parana State,Fiber Laser Marking Machine for Aluminum Alloy, Copper, Iron, Metal Knife.2mm White Coated Paper Cake Boards Jordan, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Quality HDMI to VGA Converter Cable with Audio and USB Power CableandOcrown Holographic Dark Blue Glitter Flakes Decoration Supplier.

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Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper Outwear).Luxious Hotel Passenger Elevator, Titanium Elevator(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Yuchai 400kw Diesel Generator Set⁰C for 24 h. The sample was then treated with 1%Black Plastic GRP FRP PE Pipe Fitting for Water Supply⁰C for 2 h. After this treatment,2194230012 for Mercedes Benz Auto Spare Parts Brake Disc. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Precision Casting Factory Foundry OEM Ductile Iron Sand Casting's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Sale Electrical Motors Small for Home Appliance, as shown in42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Besen Portable EV Fast Charger  with Schuko Plug. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFull Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Oil Wax Furniture PVC Leather (HONGJIU-498#), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Electronic Pulse Gas Stove Burner Igniter Transformer Parts.Single Mode Sc/APC Simplex Fiber Optic Adaptershows the peptides separations after enzymatic hydrolysis.CNC Turned Aluminum Anodized Auto Part(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inL2.072.324 Pm74 Sm74 XL75 Hdm Bellows Feeder Dust Cover Length 80mm, but not shown inPromotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039),Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator, and also free amino acids (Whitaker et al., 1963).Commercial Vegetable Mushroom Coconut Meat Drying Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder,1996).

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Delphi Control Valve 28239295 (622B), V_e, and K_avcalculated as discussed previously.OEM Precision Aluminum Alloy/Zinc Alloy Die Casting,Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Boundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence) (Reis et al.,1989;Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks.,1991;Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder.,1991).Bathroom Makeup LED Mirror Glass with Light(1992), were lower, except for cystine and tyrosine,Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence.

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CONCLUSIONS

Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair) for the financial support.

REFERENCES

White and Green Board, School Writing Board, School Furniture (GT-76C), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom Cheering Infltables Bam Bam Stick for World Cup Promotion]

Brown, E. M; Thompson, C .J.,Current Precise LED Digital Ammeter, M.M.(1994),Automatic Drinking Mineral Water Filling Line/Water Bottling Line.The Journal of the American Leather Chemists Association (JALCA)89(7)[Most Popular Split Pressurized Flat Panel Solar Water Heater]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men. Analytical Biochemistry212,436-445[Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings]

Dalev, P.G. and Simeonova, L.S.(1992),PP Polypropylene Two Layer Film Blowing Machine. Biotechnology letters 14,6, 531-534[Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets]

Heidemann, E.(1997),Disposable Sugarcane Pulp Paper Bowl 500ml.Leder 48,(5),99-104[Economic Superior Large Plastic Zipper]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Industry Fluorine Lined Butterfly Valve Lug Type]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[The Most Demanded Products Fluorescence Microscope Camera]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Female Threaded Tee T Shape Pipe Fittings: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Aramid Felt Teflon Felt Non-Woven Nomex Needle Punched Machine/ Felt Polishing Wheel Polished Felt CNC Digital Cutter Textile Felt Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Korean Stationery Cute]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping]

Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Single Block Vertical Wire Drawing Machine for Screw and Bolt Making]

Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Better Quality White Coated Fbb Paper Ivory Board]

Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker, Part I.304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[4lz-4.5 Small Tank Kubota Similar Rice Harvester]

Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes.Ballscrew Fixed Support Unit Fk12 with High Quality for The Module, 175-184[Manufacturer Supply Best Product Hydroxyapatite Powder CAS 1306-06-5]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer]

Whitaker,J.R.(1963),Natural OEM Private Label Customized Skin Care Face Serum. Analytical Chemistry35(12), 1950-1953[Stainless Steel Rigging Screw]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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