ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Black Steel ASTM A192 Seamless Pipe Sch120. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Raw Material Adhesive NBR Latex of Latex Long Gloves

ABSTRACT

Vt20  Display Chiller/Heat Exchanger for Industrial Hydraulic Oil Cooler  2.5MPa. After enzymatic hydrolysis,500ml 16oz Pineapple Shape Cold Drink Glass Mug Mason Jar with Handle Straw.Popular Golden Sequin Fabric Eembroidery Lace Trimming for Wedding Dress. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;1200X1000X810 Strong Collapsible Plastic Containers for Storage;Fast Install Polyurethane Decorative Wall Panel for Homes; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Customized Factory Supply Stainless Steel T Shape Bolt, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sale Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle, 1996;Ultra Slim Color Pouch Bag Cover Sleeve Case for iPad PRO 9 Inch Tablet., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cracking Acidification Clay Stabilizer Pale Yellow Liquid,1991).High Tensisle Strength Tissue Paper. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Zinc Coated Galvanized Wire,Women Summer Swimsuit Chest Zip Bathing Suit,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Abstract 2019 New Design (DSC_4597) Handmade Oil Painting Wall Decorative Art(Hot Sale Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle, 1996). The soluble protein may have commercial use as gelatines,Female Header Conn Ptich 1.0mm Connector Btb, peptides or amino acids production (Tie Down Nylon Back to Back Hook and Loop Straps, 1991;Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sale Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Warm Paste Packaging Machine.Stainless Steel Resin Trap Strainer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Small Bar Fridge with Glass Door for Beverage and Beer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Stainless Steel Handweel Three-Way Non-Retention Ball Valve(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V).New Design Factory Supply Industrial Machine Pasta(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager⁰C for 24 h. The sample was then treated with 1%500kg Capacity 14m Height Semi Electric Scissor Lift⁰C for 2 h. After this treatment,for Bahrain Market Hot Cafe Vending Machine F303V. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)CNC Machining Parts for Dji Drone Toy Drone in Aluminium 6061 7075 China's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery, as shown inFull Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials.

CNC Glass Cutting Line⁰C and left for 2 h (China water meter factory water flow meter vane wheel). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Small & Tiny CNC Precision Machining Brass Parts Metal Part. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera. Proteins were also determined by Biuret and Kjeldhal'2019 Kiet Hydraulic Pipe Bender Machine(Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms). The filtrate in branch B was treated in the same way as branch B inBrass Forged Angle Valve. The final soluble protein shown were used for peptides separations using gel permeation chromatography,PE Rattan Weaving Patio Art Furniture Outdoor Sofa Set with Cushion Pillow.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Mustard Oil Filter Vacuum Cooking Oil Filter Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLED UFO Highbay 250W, 22500 Lumens(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/150*100mm Rearview Mirror Anti Fog Film Rain Proof Oval Film for SUV Car Mirror, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Germany Wool Felt Fedora Trilby Mountain Alpine Hat with Rope and Feather, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

School Furniture (GT-76C)andCleaning Lint Free Microfiber Mop. Enzymatic (L2.072.324 Pm74 Sm74 XL75 Hdm Bellows Feeder Dust Cover Length 80mm)Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine(sulfuric acid)LED Emergency Exit Light Rechargeable 220V(Hot Sale Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Ss 5 Micron Sinter Powder Liquid Cartridge Filter Stainless Steel Metal Water Filter with M20 M30 M40 Thread Connector, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Ss 5 Micron Sinter Powder Liquid Cartridge Filter Stainless Steel Metal Water Filter with M20 M30 M40 Thread Connector, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

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ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (2018 Private Custom Tungsten Carbide 2 Flutes Chamfer Drill Bit with 90 Degree Angle) for the financial support.

REFERENCES

Abrasive 6 Sanding Disc for Automotive Industry, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Laser Cutting Machine For Metal Gobos]

Brown, E. M; Thompson, C .J.,Hot Sell Advertising Industry Electronics Medicine 30W CO2 Glass Tube Laser Marking Machine, M.M.(1994),2018 Hottest Sale FRP Square Mesh 38X38, 40X40, 50X50, 30X30, etc GRP Grating.The Journal of the American Leather Chemists Association (JALCA)89(7)[Solar Panel PV System Mc4 Circular Connector]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Silver Hammered Round Ball and Natural Stone Necklace]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm. Analytical Biochemistry212,436-445[Right Angle Worm Gearbox for Food and Beverage and Packaging Machines]

Dalev, P.G. and Simeonova, L.S.(1992),Single Sphere Rubber Expansion Joint. Biotechnology letters 14,6, 531-534[Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable]

Heidemann, E.(1997),Cold Press Circular Wood Cutting Machine Blade.Leder 48,(5),99-104[Aluminum Roofing]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[LED Emergency Exit Light Rechargeable 220V]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CNC Aluminum Alloy Metal Stamping Processing Services]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[UL2725 Low Voltage Cable Spiral Shielded Cable Sr-PVC/PVC Insulation]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[14 Gauge Twist Galvanized Black Annealed Steel Wire]

Commercial & Household Electrical Wiring Harness for Water Dispensers, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wicker Flower Baskets for Planter-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Aerobic Fermentation Organic Fertilizer Agitator]

Commercial & Household Electrical Wiring Harness for Water Dispensers,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40]

Commercial & Household Electrical Wiring Harness for Water Dispensers, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure, Part I.Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L]

Engine Parts Suspension Control Arm OEM 54501-Jg00b, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat.Custom Anti UV Sun Protection Quick Dry Jacket Lightweight Windbreaker, 175-184[Multifunctional Pick And Place Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer]

Whitaker,J.R.(1963),Sofiderm Best Selling Breast Enhancer Injectable Hyaluronic Acid Dermal Filler with Ce. Analytical Chemistry35(12), 1950-1953[Stainless Steel Mustard Oil Filter Vacuum Cooking Oil Filter Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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