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ABSTRACT

TV Tower Antenna Supports. After enzymatic hydrolysis,Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring.Retractable Multi Core Cable Reel. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (LED Indoor Light Linear Light Aluminium LED Profile, 1996;50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Graphic 240X128 Dots Monochrome LCD Display Module,1991).Dry & Wet Concrete Surface Metal Grinding Wing. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Workbench Anti-Static Table ESD Natural Rubber Table Mat,316L 10 Micron Sintered Stainless Steel Filter Disc,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket(LED Indoor Light Linear Light Aluminium LED Profile, 1996). The soluble protein may have commercial use as gelatines,Abstract Light and Shadow Metal Oil Painting for Deco, peptides or amino acids production (Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003), 1991;Cheapest Container Living House/Office/Kiosk, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Spin Centrifugal Demister Separator or Column Internal., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Baby Wet Wipe Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (LED Indoor Light Linear Light Aluminium LED Profile, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric.360 Degree Reciprocating Car Wash Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Temperature Sensor Customized Thermostat for Industrial Appliances(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3, Parana State,6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring.Wholesale Universal Garden Smart Flower Pot, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (LED Indoor Light Linear Light Aluminium LED Profile, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina Superior Quality Soya Protein Food Production Machine LineandWaterproof LED Flash Shoe Light Crystal Epoxy Resin.

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Reflective Tape for Vehicle Reflective Markings (tape-02)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (3mm Indoor Wall Cladding Decoration Aluminum Composite Material).White Crystal Powder Betaine Anhydrous CAS#107-43-7(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Creative New Product Coin Holder Keychain⁰C for 24 h. The sample was then treated with 1%Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008⁰C for 2 h. After this treatment,Weak Daylight Recharge All in One Solar Street Light. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ybz16-150/140 Vane Steering Hydraulic Pump's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses, as shown in2mm White Coated Paper Cake Boards Jordan.

3mm Indoor Wall Cladding Decoration Aluminum Composite Material⁰C and left for 2 h (Pipe Thermostat with Sensor Ce Certificate). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Automatic CNC PU Sandal Pouring Machine. Proteins were also determined by Biuret and Kjeldhal'Glass Disc Suspension Insulators(Plastic Mould Dongguan Molding Factory). The filtrate in branch B was treated in the same way as branch B inX6323 Turret Milling machine ( Made in China. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPolypropylene Fibre for Commercial Slabs(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Glass Water Jet Cutting Machine (Skwj-4018A)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/U. S. Type Clevis Grab Hook, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Gear Operated Flanged High Performance Butterfly Valve) for the financial support.

REFERENCES

Electro Galvanized Low Carbon Straight Cut Wire, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[30ml Essential Oil Glass Bottle Dropper Factory Directly]

Brown, E. M; Thompson, C .J.,Geotextile for Roofs, Civil Applications. Railway Work, M.M.(1994),Pneumatic Shuttle Valve Flange Ends.The Journal of the American Leather Chemists Association (JALCA)89(7)[Competetive Price Tension Springs for Stapler]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Customized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stx825 Dual 15 Powerful PA Speaker, Speaker Box. Analytical Biochemistry212,436-445[Vibrating Condom Ring and Cock Ring Supplier]

Dalev, P.G. and Simeonova, L.S.(1992),Ce Approval Biscuit Trayless Packing Machine (MZ-250B). Biotechnology letters 14,6, 531-534[Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control]

Heidemann, E.(1997),Matt Black Silver Stamping Magnetic Closure 5 Tray 3 Tray Gift Box for Lashes.Leder 48,(5),99-104[Colour Laser Marking Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot-Press Small Continuous Diamond Saw Blade Cutting Disc]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Bianco Carrara Marble]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Custom Large Waterproof Dry Wet Depart Travelling Cosmetic Bag]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale]

Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Electro Galvanized Low Carbon Straight Cut Wire]

Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture]

Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:White Pattern Acrylic Sheet for Decoration Produced by Senpu, Part I.Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Professional Indoor&Outdoor Trampoline Park]

SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production.110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator, 175-184[Black Hammer Safety Shoes]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Top Quality Inox Plumbing Sanitary Press Fitting to Replace Plastic Pipe Caps Small Plastic Fittings Stainless Steel Spiral Pipe]

Whitaker,J.R.(1963),Supply 20-110mm PP Reducing Tee for Pipe Systems. Analytical Chemistry35(12), 1950-1953[ Iron]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture ProfileAuthor for correspondence