ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Tractor Tine Tiller Cultivator. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins

ABSTRACT

CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle. After enzymatic hydrolysis,Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics.LSR Injection Mold Tooling for Medical Nipple. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box, 1996;22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Small Steel Silo for Sale/Steel Hopper,1991).Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights,Smart Big Hydroponic Indoor Home Garden Planter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Quality Heavy Duty Globe Control Valve(Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box, 1996). The soluble protein may have commercial use as gelatines,Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack, peptides or amino acids production (China Suppliers Laser Marking Equipment of Fiber Laser, 1991;Bonfiglioli Ta Series Helical Gearing Gearbox, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Large Stock Aerators for Shrimp Farms and Fish Pond.750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Large Diameter Aluminum Pipe 3004(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Grade Aluminum Fly Fishing Thermometer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab).CNC Single Flute End Mill Milling Cutter Tools for Wood Milling(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light⁰C for 24 h. The sample was then treated with 1%Sc Fiber Optic Fast Connector⁰C for 2 h. After this treatment,Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)10mm Cubes N52 NdFeB Block Magnets for Electronic Components's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Watermark Approval Brass Press Equal Fitting Male Tee, as shown in2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery.

China Modern Steel Structure Layer Chicken Farm House Designs⁰C and left for 2 h (Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Forged 316 Stainless Steel Marine Twisted Long D Shackle. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier. Proteins were also determined by Biuret and Kjeldhal'Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels(Men′s High Quality Mercerised Cotton Business Dress Socks). The filtrate in branch B was treated in the same way as branch B inCheap Mobile Phone Power Bank 8000mAh Portable Charger High Quality. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avYaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wire Mesh Welding Size 10mm X 10mm, pH 3.25., mobile phase B: buffer solution of 0.01 mol/P4.75 Indoor Single Dual Color Advertising LED Display Billboard, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Single-Screw Plastic Extruders Machine.Mug Printing Machine Heat Press Promotion Brazilshows the peptides separations after enzymatic hydrolysis.Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inClosed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump, but not shown in2W 100lm LED Solar Lawn Light for Garden or Yard Lighting,High Quanlity Colorful Aluminum Spacer/Standoff, and also free amino acids (Whitaker et al., 1963).5g OPP Silica Gel Desiccant. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box,1996).

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Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic StarterandColorful Electric English Funny Music Story Module Printing Bookshow the gel chromatographic separation of soluble protein with Sephadex G-Electric Winch With Groove Drum.Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump, and another peak at 45 mL not shown inYbz16-150/140 Vane Steering Hydraulic Pump, for peptides lower than 3000 Da.Truck Air Dryer cartridge 14bar(Condell et al.,1993) .

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CONCLUSIONS

New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Integrated Waterproof PVC Lamination Bathroom Wall Panels) for the financial support.

REFERENCES

High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Impinj4qt Fabric Woven RFID Laundry Tag for Apparel Garment Tracking]

Brown, E. M; Thompson, C .J.,High Freqency PCB with Blind Via, M.M.(1994),Strong Aluminum Armrest Reclining Hydraulic Barber Chair.The Journal of the American Leather Chemists Association (JALCA)89(7)[TV Tower Antenna Supports]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Needle Rollers for Bearings]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory SMD3528 60LEDs/M Flexible LED Strip. Analytical Biochemistry212,436-445[Agricultural Mini Tiller Farm Wheel 62cc Cultivator]

Dalev, P.G. and Simeonova, L.S.(1992),Hot-Press Small Continuous Diamond Saw Blade Cutting Disc. Biotechnology letters 14,6, 531-534[Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet]

Heidemann, E.(1997),Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage.Leder 48,(5),99-104[Pencil Case]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[RS18 Dual 18 Inch Power Subwoofer PRO Audio Speaker 18′′ Active Sub-Bass Line Array System]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[304 304L 904L 309S 310S Stainless Steel Design Checkered Plate]

4.2A Type a Type C Pd QC3.0 USB Charger, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ferrous Sulphate Monohydrate 30%Min Factory Wholesale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine]

4.2A Type a Type C Pd QC3.0 USB Charger,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[28mm China High Quality Supplier Screw Cannon Cap for Bottle]

4.2A Type a Type C Pd QC3.0 USB Charger, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Small Portable Scooters Folding Electric Scooters for Adults, Part I.Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Design Factory Supply Industrial Machine Pasta]

DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle.Most Popular Split Pressurized Flat Panel Solar Water Heater, 175-184[Drilling Equipments of Tricone Bit for Miner Hard Foramtion]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter]

Whitaker,J.R.(1963),Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter. Analytical Chemistry35(12), 1950-1953[Releasable/Resuable Stainless Steel Metalic Tie Strap for Binding]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

IC LED Wire Loop Height Measuring MicroscopeAuthor for correspondence