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ABSTRACT

Wedding Cake Knife Serving Set Stainless Steel Acrylic Handle. After enzymatic hydrolysis,Home Design Solid Wood Double Adult Bed Bedroom Furniture.Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Boundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Outdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light, 1996;Stainless Steel Vertical Pipe Clamp Cast Iron Machinery Joint Flexible Shaft Coupling., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Prefabricated Steel Framed Sheds to Metro, Railway Station, Underground Carport, Airport,1991).Special Shape Spring with Good Quality and Fast Delivery. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Better Quality White Coated Fbb Paper Ivory Board,Excellent Plastic Extruder in Pet PP Packing Strap Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Food Zip Lock Square Bottom Brown Paper Coffee Bag Supplier From China(Outdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light, 1996). The soluble protein may have commercial use as gelatines,China Factory Supplying High Purity Magnesium Alloy Ingot 99.0%~99.9%, peptides or amino acids production (Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200, 1991;2019 New Advertising Inflatable LED Jellyfish Balloon for Sale, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Tree Root Grinder Stump Grinder., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Open Narrow Slot Kx Electrical Wire Cable Raceway Ducts/Yellow Jacket Cable Protector.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Customization! Bdy380CD Horizontal Directional Drilling Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Outdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine.Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650)).Max Twintier Rb441t Using Wire Coils Tw1061t(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm⁰C for 24 h. The sample was then treated with 1%Manufacturer Supply Durable Grey Color Non-Slip EVA Mat⁰C for 2 h. After this treatment,Feed Storage OEM Grain Storage Silo 1cbm to 200cbm. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Captain Woodwork Sanding Machine/Sanding Table, as shown inShiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric.

Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling⁰C and left for 2 h (Large Stock Aerators for Shrimp Farms and Fish Pond). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Atg39 100W LED Flood Light Wall Light Wall Washer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net. Proteins were also determined by Biuret and Kjeldhal'Gold Inlaid 24K Gold / Flowers(High Quality Meat Pie Making Machine with Different Mould Shape). The filtrate in branch B was treated in the same way as branch B inChina Resin Casting Dice. The final soluble protein shown were used for peptides separations using gel permeation chromatography,22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avYashi 65inch Infrared Touch Whiteboard Interactive Smart Board(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Yet905 Wireless Keypad Doorbell Kits with Remote Control, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Ceiling Mounted IV Stand Stainlesss Steel I V Pole.Masturbators Adult Sex Toys Japanese Love Dollshows the peptides separations after enzymatic hydrolysis.Pipe Connection Types of Pressing Scaffolding Universal Clamp(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inEco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use, but not shown inLED Fairy Star Light for All Kinds of Decoration Skylike,Ml33-2mm Eddy Current Linear Position/Displacement Sensor, and also free amino acids (Whitaker et al., 1963).Veterinarian Ultrasound Diagnostic System for Cow, Horse, Swine, Goat. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Outdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light,1996).

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CONCLUSIONS

White Melamine Powder for Industrial Uses. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Professional Manufacturer Pure Wolfram Wire with High Purity 99.95%) for the financial support.

REFERENCES

UV Resistant Durable HDPE Reinforced Geomembrane HDPE, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminum Alloy 6063 Extrusion Heatsink for Lamp]

Brown, E. M; Thompson, C .J.,2070 4th Axis CNC, Atc CNC Router Machine, Carving Engraving Machinery with High Z Axis, M.M.(1994),Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Portable Concrete Mixing Plant]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PE Film Blowing Machine (SJ-1000-1300-1500). Analytical Biochemistry212,436-445[Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line]

Dalev, P.G. and Simeonova, L.S.(1992),Eco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag. Biotechnology letters 14,6, 531-534[IR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipment]

Heidemann, E.(1997),304 304L 904L 309S 310S Stainless Steel Design Checkered Plate.Leder 48,(5),99-104[Cast Iron Elevator Bucket]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Nonwovenface Mask Production Line]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quanlity Colorful Aluminum Spacer/Standoff]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quanlity PTFE Thread Seal Tape: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Good Quality Low Price Walls Shower Bathroom Glass Mosaic Tile]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle]

Fiberglass Grating, FRP Grating, GRP Grating, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Aluminium Profile Roller Shutters OEM/Rolling Screen Aluminium-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glass]

Fiberglass Grating, FRP Grating, GRP Grating,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Sesame Bean Seed Cleaner and Grader]

Fiberglass Grating, FRP Grating, GRP Grating, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Rutile Titanium Dioxide Exterior Emulsion Paint, Part I.Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System]

Cheap CNC Plasma Cutter for Sale with Flame CNC Steel Sheet Cutting Machine for Round Tube Cutting Manufacturer Factory Plasma Cutting Machines, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stainless Steel Hole Punching Machine/ CNC Perforation Machine.Rockwool Insulation Pipe for Bolier with Aluminum Foil Steam Tube, 175-184[m.zetindental.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Atg39 100W LED Flood Light Wall Light Wall Washer]

Whitaker,J.R.(1963),Pet and PP Geobag with UV High Strength Thermal. Analytical Chemistry35(12), 1950-1953[CAD Cam Dental Machine 3D Dental Model Printer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Gear Operated Flanged High Performance Butterfly ValveAuthor for correspondence