ARTICLES

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ABSTRACT

Black Steel ASTM A192 Seamless Pipe Sch120. After enzymatic hydrolysis,Raw Material Adhesive NBR Latex of Latex Long Gloves.Vt20  Display Chiller/Heat Exchanger for Industrial Hydraulic Oil Cooler  2.5MPa. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.1200X1000X810 Strong Collapsible Plastic Containers for Storage, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fast Install Polyurethane Decorative Wall Panel for Homes, 1996;Customized Factory Supply Stainless Steel T Shape Bolt., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sale Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle,1991).Ultra Slim Color Pouch Bag Cover Sleeve Case for iPad PRO 9 Inch Tablet. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cracking Acidification Clay Stabilizer Pale Yellow Liquid,High Tensisle Strength Tissue Paper,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Zinc Coated Galvanized Wire(Fast Install Polyurethane Decorative Wall Panel for Homes, 1996). The soluble protein may have commercial use as gelatines,Women Summer Swimsuit Chest Zip Bathing Suit, peptides or amino acids production (Abstract 2019 New Design (DSC_4597) Handmade Oil Painting Wall Decorative Art, 1991;Female Header Conn Ptich 1.0mm Connector Btb, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Tie Down Nylon Back to Back Hook and Loop Straps., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fast Install Polyurethane Decorative Wall Panel for Homes, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks.Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Warm Paste Packaging Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Lithium Battery 20ah Pouch 3c Cell 3.7V(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Heavy Duty Steel Warehouse Storage Pallet Rack).Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stainless Steel Handweel Three-Way Non-Retention Ball Valve⁰C for 24 h. The sample was then treated with 1%New Design Factory Supply Industrial Machine Pasta⁰C for 2 h. After this treatment,Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)500kg Capacity 14m Height Semi Electric Scissor Lift's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)for Bahrain Market Hot Cafe Vending Machine F303V, as shown in120PCS/M Flexible LED Strip Tira De LED Light Strip.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2019 Kiet Hydraulic Pipe Bender Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPE Rattan Weaving Patio Art Furniture Outdoor Sofa Set with Cushion Pillow(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.LED UFO Highbay 250W, 22500 Lumens⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Nonstick Vegetable Oil/Cast Iron Bakeware BSCI LFGB FDA Approved, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Single Mode Sc/APC Simplex Fiber Optic Adapter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player, Mirror Link and Built-in Bluetooth Car Radio Car Player) for the financial support.

REFERENCES

120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Good Price High Quality Image Blue/ Black 55/ 50/ 55GSM Carbonless Paper/ NCR]

Brown, E. M; Thompson, C .J.,Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine, M.M.(1994),Abrasive 6 Sanding Disc for Automotive Industry.The Journal of the American Leather Chemists Association (JALCA)89(7)[IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Sell Advertising Industry Electronics Medicine 30W CO2 Glass Tube Laser Marking Machine. Analytical Biochemistry212,436-445[Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes]

Dalev, P.G. and Simeonova, L.S.(1992),2018 Hottest Sale FRP Square Mesh 38X38, 40X40, 50X50, 30X30, etc GRP Grating. Biotechnology letters 14,6, 531-534[Feed Storage OEM Grain Storage Silo 1cbm to 200cbm]

Heidemann, E.(1997),Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm.Leder 48,(5),99-104[Cast Steel Sow Mold Factory OEM/ODM Products]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush]

Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Single Sphere Rubber Expansion Joint.Cold Press Circular Wood Cutting Machine Blade. USP/SP[Experienced and Professional Plastic Helmet Mold Manufacturer]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Petrochemical Loading Platform Folding Stairs]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Galvanized Trench Cover: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hot Sale 3X4.5 Easy Pop up Folding Tent]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag]

Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth]

Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Mooncake Pastry and Plastic Packaging Box]

Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Commercial & Household Electrical Wiring Harness for Water Dispensers, Part I.Wicker Flower Baskets for Planter. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Safe Delivery Effective Sarms Mk 677 for Bodybuilding]

5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre.Engine Parts Suspension Control Arm OEM 54501-Jg00b, 175-184[24-Hour Hot Line]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[IP65 Canopy LED Light Fixture 150W LED Gas Station Light]

Whitaker,J.R.(1963),Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat. Analytical Chemistry35(12), 1950-1953[Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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