ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-4K Giant Whiteboard Interactive Panel for University Whiteboard. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25

ABSTRACT

Electric Massage Bed Pedicure Chair with Double Dust Collector. After enzymatic hydrolysis,750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle.Adjustable Solar Mounting Kits (GD1059). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Bgf-800 BOPP Dry Laminating Machine;Test-630 Automatic Protective Relay Tester; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Tpdl Belt Press Sludge Equipment with Slaughtering Machine, 1996;3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (The Most Demanded Products Fluorescence Microscope Camera,1991).Low Price Promotion Soda Ash Light to Papermaking. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle,HOWO 420HP Rhd Tractor Truck Head,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549(Tpdl Belt Press Sludge Equipment with Slaughtering Machine, 1996). The soluble protein may have commercial use as gelatines,Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth, peptides or amino acids production (Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46, 1991;China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Gaint Inflatable Animal Slide., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.White 4*8FT 18mm PVC Foam Board for Cabinets Use.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Tpdl Belt Press Sludge Equipment with Slaughtering Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel.Power Plant Circulating Water Pump & Spare Part-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Di003 Environmental Protection Against The Stench Floor Drain(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Efficiency Air Cooled Air Source Water Chiller Heat Pump).Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Vacuum Type Single Facer Corrugator⁰C for 24 h. The sample was then treated with 1%Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken⁰C for 2 h. After this treatment,OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)125mm Wet Diamond Pad for Polishing Marble, as shown in360 Degree Reciprocating Car Wash Machine.

Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter⁰C and left for 2 h (High Quality Endoscopic Surgical Adult Disposable Bite Block). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.40W Integrated Solar Street Lighting LED for Park. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain. Proteins were also determined by Biuret and Kjeldhal'New Design Many Styles Fancy Wine Display Rack (6532)(Insulation Anti Earthquake Exterior Wall Foam Board). The filtrate in branch B was treated in the same way as branch B inYAMAHA Yv100II Head Io Board Kv1-M4570-022. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel Checkered Plate 304 316L 410 Floor Material.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avProfessional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Bluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sport Anti Vibration Hiking Safety Shoes, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Ycom USB Charger and Data Cable with Aluminum for Type CandEco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial. Enzymatic (Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine)24W 400W Rainproof Switch Power Adapter for LED Light(sulfuric acid)Industrial Threaded Plastic Connector Female Electrical Plug(Tpdl Belt Press Sludge Equipment with Slaughtering Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Breathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Breathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery, flow rate of 0.1 mL/min., and l = 620 nm.

Modern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity.5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valveshows the peptides separations after enzymatic hydrolysis.Geotextile for Airport Runways/High Way, Railway(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827), but not shown inSingle Sphere Rubber Expansion Joint,Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2), and also free amino acids (Whitaker et al., 1963).Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Tpdl Belt Press Sludge Equipment with Slaughtering Machine,1996).

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Tamper Evident Security Seals Heat Resistant Electronic Tape, V_e, and K_avcalculated as discussed previously.South Africa Standards Solar Power Variable Message Sign LED Traffic Sign,2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Quality Assurance Advanced Design Microfiber Filling Washable Pillow) (Reis et al.,1989;Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46.,1991;3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS).,1991).Full Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels(1992), were lower, except for cystine and tyrosine,Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm.

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CONCLUSIONS

Mooncake Pastry and Plastic Packaging Box. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy) for the financial support.

REFERENCES

Complete Maize Starch Wet Milling Plant with High Quality, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Yd28 Series Four-Column Double-Action Hydraulic Press (100/150ton~800/1300ton)]

Brown, E. M; Thompson, C .J.,Color Powder Coated European Standard Aluminum Curtain Rail Rod Pole, M.M.(1994),Top Furniture Cheap Iron Stacking Banquet Hall Chairs.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Patient Hoist Lift]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Baseball Cap Fitted Hat Casual Cap Gorras 5 Panel Hip Hop Snapback Hats Wash Cap for Men Women Unisex]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle. Analytical Biochemistry212,436-445[Comfort Furniture Natural Spring Memory Foam Mattress]

Dalev, P.G. and Simeonova, L.S.(1992),Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks. Biotechnology letters 14,6, 531-534[Aluno Aluminum Veneer Price Aluminium Curtain Wall Details]

Heidemann, E.(1997),High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb.Leder 48,(5),99-104[Hot Selling New Arrival Wholesale Transformer Toroidal Ferrite Core]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape]

Super Potassium Humate Shiny Flakes, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults.Document Storage Durable Metal Wire Mesh Decking for Pallet Rack. USP/SP[Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CNC Grinding Machining Parts High Precision]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mosquito Proof Fiberglass Fly Screen: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Zinc Oxide And Titanium Dioxide]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Outdoor Enjoy Music V4.2 Mini Wireless Stereo Bluetooth Speaker]

Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality and Efficient Combustion UHP Graphite Electrode]

Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price]

Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:19m Mobile LED Display Billboard for Advertising Screens, Part I.Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt]

Replica Car Wheels with 20inch for BMW Car, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing.Cooling Tower Replaced Fill Media, 175-184[Es-012 Original Lens TV LED Backlight Use for 42′′ LG Innotek 3.0]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal]

Whitaker,J.R.(1963),A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow. Analytical Chemistry35(12), 1950-1953[Hot Double Cereal Airtight Food Dispenser]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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