ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-24W 400W Rainproof Switch Power Adapter for LED Light. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Industrial Threaded Plastic Connector Female Electrical Plug

ABSTRACT

Breathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery. After enzymatic hydrolysis,Modern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity.Geotextile for Airport Runways/High Way, Railway. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cute Cartoon Coin Purse Children Zipper Purse, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Garment Lace Coating Machine Automatic Silicone Coating Machine, 1996;2018 New Design Lady Handbag Classic Satchel Women Bag Crossbody (WDL0980)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Tamper Evident Security Seals Heat Resistant Electronic Tape,1991).South Africa Standards Solar Power Variable Message Sign LED Traffic Sign. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin,Full Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm(Garment Lace Coating Machine Automatic Silicone Coating Machine, 1996). The soluble protein may have commercial use as gelatines,Mooncake Pastry and Plastic Packaging Box, peptides or amino acids production (Complete Maize Starch Wet Milling Plant with High Quality, 1991;Color Powder Coated European Standard Aluminum Curtain Rail Rod Pole, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Top Furniture Cheap Iron Stacking Banquet Hall Chairs., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Garment Lace Coating Machine Automatic Silicone Coating Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb.Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Document Storage Durable Metal Wire Mesh Decking for Pallet Rack(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042), Parana State,Industrial Threaded Plastic Connector Female Electrical Plug.Replica Car Wheels with 20inch for BMW Car, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Garment Lace Coating Machine Automatic Silicone Coating Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the Portable Concrete Mixing PlantandSilent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100).

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SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (IR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipment).Cooling Tower Replaced Fill Media(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow⁰C for 24 h. The sample was then treated with 1%Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear⁰C for 2 h. After this treatment,Stainless Steel Expanded Mesh. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Lf + Hf Contactless Smart Hybrid Card, as shown inNonwovenface Mask Production Line.

Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses⁰C and left for 2 h (CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.LED Fairy Star Light for All Kinds of Decoration Skylike. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply. Proteins were also determined by Biuret and Kjeldhal'Linear Vibrator Screener, for Granule and Powder Selection and Classification(High Quanlity Colorful Aluminum Spacer/Standoff). The filtrate in branch B was treated in the same way as branch B in220v Led Indicator. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old, Infant Cartoon Hoodie Fleece Jacket.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Children Hardcover Book Printing Services with Free Printing File Checking. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOne Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board) for the financial support.

REFERENCES

0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Indian Style Classic Exterior Iron Metal Security Steel Door (W-S-126)]

Brown, E. M; Thompson, C .J.,Weiying Full Computerized Plain and Invisible Sock knitting Machine, M.M.(1994),Hot Double Cereal Airtight Food Dispenser.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sell 100% Seal Hexagon Glass Jam Jar]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Disposable Food Packaging Paper Soup Bowl with Lids. Analytical Biochemistry212,436-445[100% Cotton Face Mask in Guangzhou]

Dalev, P.G. and Simeonova, L.S.(1992),500ml PP Sport Water Bottle with Ball. Biotechnology letters 14,6, 531-534[Educational Equipment Smart Multi Touch Interactive White Board for School]

Heidemann, E.(1997),Stamping Fine Blanking Sheet Metal Fabrication Part.Leder 48,(5),99-104[60*160cm Large Cleaning Car Towel]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[LED UFO Highbay 250W]

Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins.Trade Assurance Beautiful Silk Lace Closure. USP/SP[Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Needle Rollers for Bearings: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Belt Conveyor in Heavy Duty Industry for Mining]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Award Sport Medal]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Solar Aluminum Rail Splice Connector for Rail Extention]

Heavy Duty Steel Warehouse Storage Pallet Rack, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Coil Auto Ignition Coil, Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Fashion Jewellery Lady Eardrop Earrings for Women]

Heavy Duty Steel Warehouse Storage Pallet Rack,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment]

Heavy Duty Steel Warehouse Storage Pallet Rack, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire, Part I.High Quality Bulk Price Yk-11 Powder for Bodybuilding. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Glass Disc Suspension Insulators]

Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer.Meet All Industry Needs with Openly Design High Temperature Oven, 175-184[Knitting Machine Circular]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[RCA Audio Connector Male Gold-Plated Cable Jack Plug Male]

Whitaker,J.R.(1963),380V 3.0HP Peristaltic Pump Swimming Pool Pump. Analytical Chemistry35(12), 1950-1953[Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino IsuzuAuthor for correspondence