ARTICLES

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ABSTRACT

220V/50Hz Stainless Steel Ozone Generator with 5g/H. After enzymatic hydrolysis,Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training.Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (100% Cotton Face Mask in Guangzhou, 1996;OEM High Pressure Machined Part Die Aluminum Casting., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (AISI 430 Ba/2b Embossed Stainless Steel Sheet,1991).High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Drug Delivery ABS Hospital Medical Treatment Medicine Cart,YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress(100% Cotton Face Mask in Guangzhou, 1996). The soluble protein may have commercial use as gelatines,Colorful Electric English Funny Music Story Module Printing Book, peptides or amino acids production (2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine, 1991;IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Tilt-and-Turn-with-Hidden-Hinge., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Soft PVC Granule Used for Refrigerator Door Gasket.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-0.45um Dia 47mm PVDF Filter Membrane. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (100% Cotton Face Mask in Guangzhou, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Commecial Use Watch Display Cabinet for Watch Store Interior Design.Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Slotted Round Head Aluminum Self Tapping Screw(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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4 Different Ball Silicone Ice Cube Maker(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile).High Quality Washable Durable Hotel Polyester Banquet Table Cloths(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female⁰C for 24 h. The sample was then treated with 1%Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe⁰C for 2 h. After this treatment,200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Aerobic Fermentation Organic Fertilizer Agitator, as shown inHot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRecyclable PU Leather Jewelry Pouch(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Activated Alumina Used for Catalyst Carrier in Chemical Industry⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality 45W 50W Commercial Recessed COB LED Downlight, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Indoor Inkjet Media Material.China PCBA Supplier Custom Game Controller PCBshows the peptides separations after enzymatic hydrolysis.Steel Jacket Steam Insulation Pipeline Production Famouse in China(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLuxious Hotel Passenger Elevator, but not shown inPPR PVC Polyurethane Insulated Pipe Extrusion Production Machine,Solid Steel Hotel Safe (G-42BD classics) for Hotel, and also free amino acids (Whitaker et al., 1963).Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (100% Cotton Face Mask in Guangzhou,1996).

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2000LTR Horizontal Foam Tank Fire Fighting Suppression, V_e, and K_avcalculated as discussed previously.High Spped Weaving Machine 6,Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality D400 FRP Composite Lockable Waterproof Round Manhole Cover and Frame BS En124) (Reis et al.,1989;2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine.,1991;OEM High Pressure Machined Part Die Aluminum Casting.,1991).Small Poultry Pig Feed Farm Steel Silo for Sale(1992), were lower, except for cystine and tyrosine,Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine.

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CONCLUSIONS

P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker) for the financial support.

REFERENCES

Made in China 12 Inch Aluminum Flexible Duct Pipe, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Plastic Steel]

Brown, E. M; Thompson, C .J.,18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring, M.M.(1994),Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food.The Journal of the American Leather Chemists Association (JALCA)89(7)[Heavy Duty Steel Warehouse Storage Pallet Rack]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[120PCS/M Flexible LED Strip Tira De LED Light Strip]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Safe Delivery Effective Sarms Mk 677 for Bodybuilding. Analytical Biochemistry212,436-445[Grade Aluminum Fly Fishing Thermometer]

Dalev, P.G. and Simeonova, L.S.(1992),12# X 14# Electric Galvanized Double Strands Barbed Wire. Biotechnology letters 14,6, 531-534[600mm Warehouse No Power Roof Fan]

Heidemann, E.(1997),OEM Service Machining Milling Turning Service Custom CNC Aluminum Case.Leder 48,(5),99-104[m.essiai.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[12 Teeth Green Painting Steel Rake]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Super Potassium Humate Shiny Flakes: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Style Custom Logo Makeup Multi Colored Eyeshadow Palette]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Beer Water Air Pump Silicone Tubing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Stc-001/Stc-100A/Stc/8080A+]

4K Giant Whiteboard Interactive Panel for University Whiteboard, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation]

4K Giant Whiteboard Interactive Panel for University Whiteboard,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Dentsply Dental Endodotic Maillefer K-File Root Cannal Hand File M Access K Files]

4K Giant Whiteboard Interactive Panel for University Whiteboard, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Electric Massage Bed Pedicure Chair with Double Dust Collector, Part I.750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ce Approval Biscuit Trayless Packing Machine (MZ-250B)]

Adjustable Solar Mounting Kits (GD1059), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Bgf-800 BOPP Dry Laminating Machine.Test-630 Automatic Protective Relay Tester, 175-184[Fitness And Yoga Wear]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Commercial Vegetable Mushroom Coconut Meat Drying Machine]

Whitaker,J.R.(1963),Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle. Analytical Chemistry35(12), 1950-1953[Solid Steel Hotel Safe (G-42BD classics) for Hotel]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Carbon Steel Pipe ASTM A106 A53Author for correspondence