ARTICLES

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ABSTRACT

Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes. After enzymatic hydrolysis,Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting.Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wind Protection Screen. Wind Dust Netting, Wind Dust Net, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (24K Gold Rolling Paper Shine Papers Cigarette Paper, 1996;Two - Way Double Row Thrust Tapered Roller Bearings., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest,1991).Ride on Toys Riding Animals in The Mall. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Finely Processed Pneumatic Operated Globe Valve,Color Coated Corrugated Galvanized Roofing Steel Plate,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting(24K Gold Rolling Paper Shine Papers Cigarette Paper, 1996). The soluble protein may have commercial use as gelatines,Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms, peptides or amino acids production (Precision Diamond Wire Saw Rope for Cutting Concrete Stone, 1991;Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Blince High Torque OMR Hydro Motor for Injection Moulding Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-TV Tower Antenna Supports. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (24K Gold Rolling Paper Shine Papers Cigarette Paper, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring.Retractable Multi Core Cable Reel-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Xcm 120 Asphalt Mixing Plant for Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Baby Wet Wipe Machine⁰C and left for 2 h (CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric. Proteins were also determined by Biuret and Kjeldhal'360 Degree Reciprocating Car Wash Machine(Single Block Vertical Wire Drawing Machine for Screw and Bolt Making). The filtrate in branch B was treated in the same way as branch B inAutomatic Pillow Flow Packing Machine for Food/Daily. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Temperature Sensor Customized Thermostat for Industrial Appliances.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Die Aluminum Castings. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Meat Pie Making Machine with Different Mould Shape(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale Universal Garden Smart Flower Pot, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Ybz16-150/140 Vane Steering Hydraulic Pump.4lz-4.5 Small Tank Kubota Similar Rice Harvestershows the peptides separations after enzymatic hydrolysis.2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNon-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer, but not shown inStainless Steel Rigging Screw,3mm Indoor Wall Cladding Decoration Aluminum Composite Material, and also free amino acids (Whitaker et al., 1963).240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (24K Gold Rolling Paper Shine Papers Cigarette Paper,1996).

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CONCLUSIONS

Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (LED Indoor Light Linear Light Aluminium LED Profile) for the financial support.

REFERENCES

Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Heat-Proof Fire Resistant Silicone Coated Fiberglass Hydraulic Hose Guard]

Brown, E. M; Thompson, C .J.,Car Trunk Organizer Car Storage Organizer, M.M.(1994),Fashion Cotton Baby/Kid Hat (DH-BF332).The Journal of the American Leather Chemists Association (JALCA)89(7)[Customized Frame Mount Highway Pegs Enclosure Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Factory Supplying High Purity Magnesium Alloy Ingot 99.0%~99.9%]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line. Analytical Biochemistry212,436-445[Global Brands 10 Year Cheaper Hook & Loop Tape Male Side]

Dalev, P.G. and Simeonova, L.S.(1992),OEM Round Stainless Steel Fruit Plate Tray. Biotechnology letters 14,6, 531-534[Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd]

Heidemann, E.(1997),Factory Supply Industry Grade Sodium Gluconate.Leder 48,(5),99-104[Nail Art Drawing UV Spider Metal Gel Acrylic Lace String Painting Gel Supply]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Transmission Ultrasound Gel /20ml]

Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set.En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube. USP/SP[Cheap 20tons LPG Semi Trailers Gas Tank Transport Trailer for Sale]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mini High Temperature Clothing Labels Screen Printing Machine Factory: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.goldenvision-lcd.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[U. S. Type Clevis Grab Hook]

Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc., M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Quality Control High Purity 99% Ceftiofur Sodium Powder CAS104010-37-9 in Stock]

Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc.,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Mellow OEM Plastic Pet House Ml-HP004]

Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc., M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts, Part I.Conventional Best-Selling Household Bamboo Craft Chopsticks. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile]

High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ce Marked Medical Syringe Pump (WP08N).New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty, 175-184[Steel Bar Rib Peeling Thread Making Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers]

Whitaker,J.R.(1963),Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet. Analytical Chemistry35(12), 1950-1953[Ss 5 Micron Sinter Powder Liquid Cartridge Filter Stainless Steel Metal Water Filter with M20 M30 M40 Thread Connector]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Geotextile for RoofsAuthor for correspondence