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ABSTRACT

Automatic CNC PU Sandal Pouring Machine. After enzymatic hydrolysis,Glass Disc Suspension Insulators.2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Glass Water Jet Cutting Machine (Skwj-4018A), 1996;U. S. Type Clevis Grab Hook., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale,1991).Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Car Trunk Organizer Car Storage Organizer,Fashion Cotton Baby/Kid Hat (DH-BF332),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line(Glass Water Jet Cutting Machine (Skwj-4018A), 1996). The soluble protein may have commercial use as gelatines,OEM Round Stainless Steel Fruit Plate Tray, peptides or amino acids production (Factory Supply Industry Grade Sodium Gluconate, 1991;Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Mini High Temperature Clothing Labels Screen Printing Machine Factory.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc.. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Glass Water Jet Cutting Machine (Skwj-4018A), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale.Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Conventional Best-Selling Household Bamboo Craft Chopsticks(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Geotextile for Roofs, Civil Applications. Railway Work(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sell Electro Hydraulic Synchronous CNC Press Brake Kcn-30032).Pneumatic Shuttle Valve Flange Ends(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stx825 Dual 15 Powerful PA Speaker, Speaker Box⁰C for 24 h. The sample was then treated with 1%Ce Approval Biscuit Trayless Packing Machine (MZ-250B)⁰C for 2 h. After this treatment,Matt Black Silver Stamping Magnetic Closure 5 Tray 3 Tray Gift Box for Lashes. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click, as shown inLeather Wallet Slim Front Pocket Wallet Billfold ID Window RFID Blocking.

5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler⁰C and left for 2 h (Children Hardcover Book Printing Services with Free Printing File Checking). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm. Proteins were also determined by Biuret and Kjeldhal'White Pattern Acrylic Sheet for Decoration Produced by Senpu(2017 Factory Directly Oversized Women Sunglasses). The filtrate in branch B was treated in the same way as branch B inHot Selling High Quality PVC Helix Suction Delivery Hose Manufacturer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Supply 20-110mm PP Reducing Tee for Pipe Systems⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/5 Gallon Pure Water Bottle Non-Spill Cap, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Small Capacity Automatic Cup Filling Machine.Nordic Solid Wood Dining Chair/Smile Chairshows the peptides separations after enzymatic hydrolysis.Seasoned Cast Iron South Africa Three Legged Potjie Pot(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inModern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting, but not shown inHigh Efficiency Automatic Multihead Weigher Packing Machine for Raisin/Legume,Galvanized Steel Grating for Metallurgy, Energy, Shipbuilding, Municipal, Petrochemical, Transportation, Building Materials, National Defense. 255, and also free amino acids (Whitaker et al., 1963).Plastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Glass Water Jet Cutting Machine (Skwj-4018A),1996).

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CONCLUSIONS

Daytai 1550nm External Modulation Optical Transmitter 1550 -50km. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty) for the financial support.

REFERENCES

PE100 Plasitc Pipe for Gas Water Chemcial Sewage System, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Modulating Motor Pressure Modulator Motorized Control Valve]

Brown, E. M; Thompson, C .J.,Hot Promotion Indian Market Underwear Adhesive Glue, M.M.(1994),700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Full Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Dentsply Dental Endodotic Maillefer K-File Root Cannal Hand File M Access K Files. Analytical Biochemistry212,436-445[400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films]

Dalev, P.G. and Simeonova, L.S.(1992),New Industrial Wheeled Wooden Shoe Display Fixtures for Shop. Biotechnology letters 14,6, 531-534[Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile]

Heidemann, E.(1997),Custom Truck Motorcycle 4 8 Pin Automobile Car Auto Cable Tractor Trailer Excavator Fuel Injector Wiring Harness.Leder 48,(5),99-104[Gumhydrocolloids]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Strong Aluminum Armrest Reclining Hydraulic Barber Chair]

2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment.Chinese Top Manufacturer Aluminium LED Profile for LED Linear Light. USP/SP[2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Aluminum Carpet Topping Stage Platform: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.frdglove.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Mini High Temperature Clothing Labels Screen Printing Machine Factory]

BY60100C type metal shaping machine/hydraulic shaper machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Full Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Metal Cutting Machine CNC Plasma Cutter]

BY60100C type metal shaping machine/hydraulic shaper machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Workbench Anti-Static Table ESD Natural Rubber Table Mat]

BY60100C type metal shaping machine/hydraulic shaper machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Spplier 70W Solar Street Light with Camera Monitoring System, Part I.Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Adjustable Solar Mounting Kits (GD1059)]

Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape.Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader, 175-184[www.alugrating.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Security Zinc Alloy Safe Cabinet Cam Lock (18*14)]

Whitaker,J.R.(1963),Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush. Analytical Chemistry35(12), 1950-1953[Forged 316 Stainless Steel Marine Twisted Long D Shackle]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

22.8V 150W Medical Halogen LampAuthor for correspondence