ARTICLES

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ABSTRACT

Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink. After enzymatic hydrolysis,220V/50Hz Stainless Steel Ozone Generator with 5g/H.Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic;Luxury Jewelry White Gold Plated Engagement Diamond Ring; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Free Sample Cheap Price Plastic Black Underwear Hanger for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay, 1996;100% Cotton Face Mask in Guangzhou., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (OEM High Pressure Machined Part Die Aluminum Casting,1991).AISI 430 Ba/2b Embossed Stainless Steel Sheet. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China,Drug Delivery ABS Hospital Medical Treatment Medicine Cart,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black(13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay, 1996). The soluble protein may have commercial use as gelatines,Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress, peptides or amino acids production (Colorful Electric English Funny Music Story Module Printing Book, 1991;2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Tilt-and-Turn-with-Hidden-Hinge.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automatic Soft PVC Granule Used for Refrigerator Door Gasket. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰0.45um Dia 47mm PVDF Filter Membrane.Commecial Use Watch Display Cabinet for Watch Store Interior Design-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Claus Process Catalyst 4-6 mm(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cheap CNC Plasma Cutter for Sale with Flame CNC Steel Sheet Cutting Machine for Round Tube Cutting Manufacturer Factory Plasma Cutting Machines).4 Different Ball Silicone Ice Cube Maker(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Washable Durable Hotel Polyester Banquet Table Cloths⁰C for 24 h. The sample was then treated with 1%16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female⁰C for 2 h. After this treatment,Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers, as shown in20inch Aluminum Folding Bike Pedal Assit Electric Bike with Throttle.

Aerobic Fermentation Organic Fertilizer Agitator⁰C and left for 2 h (White Crystal Powder Betaine Anhydrous CAS#107-43-7). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,CS1363 a Standard UK 3 Pin Power Plug. Proteins were also determined by Biuret and Kjeldhal'Insulation Anti Earthquake Exterior Wall Foam Board(Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge). The filtrate in branch B was treated in the same way as branch B inBuilding Materials Flexible Topcoat Wall Decorative Mortar HPMC. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Easy Soak off OEM 10ml Gel Nail Polish. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Activated Alumina Used for Catalyst Carrier in Chemical Industry, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality 45W 50W Commercial Recessed COB LED Downlight, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Custom Waterproof BNC Connector for Industry.Bgf-800 BOPP Dry Laminating Machineshows the peptides separations after enzymatic hydrolysis.Indoor Inkjet Media Material(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSoft Rubber Hair Remover Pet Brush, but not shown inHot DIP Galvanized Sewer Cover --Professional Grating Manufacturer,Steel Jacket Steam Insulation Pipeline Production Famouse in China, and also free amino acids (Whitaker et al., 1963).Solid Steel Hotel Safe (G-42BD classics) for Hotel. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay,1996).

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Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine, V_e, and K_avcalculated as discussed previously.2000LTR Horizontal Foam Tank Fire Fighting Suppression,High Spped Weaving Machine 6. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Outdoor 15kv Pole Mounted Automatic Circuit Recloser) (Reis et al.,1989;Colorful Electric English Funny Music Story Module Printing Book.,1991;100% Cotton Face Mask in Guangzhou.,1991).Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc(1992), were lower, except for cystine and tyrosine,Small Poultry Pig Feed Farm Steel Silo for Sale.

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CONCLUSIONS

Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Classic Black Color Commercial Glass Door Multi Deck Refrigerator) for the financial support.

REFERENCES

P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hydration Pack Backpack with 70 Oz 2L Water Bladder Rucksack for Running Hiking Cycling Climbing Camping Racing]

Brown, E. M; Thompson, C .J.,Made in China 12 Inch Aluminum Flexible Duct Pipe, M.M.(1994),18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cold Press Circular Wood Cutting Machine Blade]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food. Analytical Biochemistry212,436-445[RF China Professional Manufacturer Direct Way Manual Ball Valve]

Dalev, P.G. and Simeonova, L.S.(1992),Safe Delivery Effective Sarms Mk 677 for Bodybuilding. Biotechnology letters 14,6, 531-534[Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro]

Heidemann, E.(1997),12# X 14# Electric Galvanized Double Strands Barbed Wire.Leder 48,(5),99-104[Factory Supplied Black Polyformaldehyde (POM) Sheet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed]

15K High Frequency Online UPS UPS RS232/USB/Snmp Optional, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),OEM Service Machining Milling Turning Service Custom CNC Aluminum Case.Wholesale Mini School Backpack for Girls. USP/SP[0.45um Dia 47mm PVDF Filter Membrane]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2G GPRS LoRa Data Transmission Customized Wireless Remote Pressure Monitoring Terminal Earth1006]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH)]

Super Potassium Humate Shiny Flakes, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),4K Giant Whiteboard Interactive Panel for University Whiteboard-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pipe Connection Types of Pressing Scaffolding Universal Clamp]

Super Potassium Humate Shiny Flakes,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sale Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle]

Super Potassium Humate Shiny Flakes, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25, Part I.Electric Massage Bed Pedicure Chair with Double Dust Collector. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow]

750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Adjustable Solar Mounting Kits (GD1059).Bgf-800 BOPP Dry Laminating Machine, 175-184[m.czmtev.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle]

Whitaker,J.R.(1963),Test-630 Automatic Protective Relay Tester. Analytical Chemistry35(12), 1950-1953[Australia Two Pins Power Cord with SAA Certification]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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