ARTICLES

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ABSTRACT

China Professional Shipping Agent to Turkey. After enzymatic hydrolysis,White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment.Crusher for Animal Waste/ Chicken Manure. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs;Threaded Locking Circular Connectors M16 680; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card, 1996;Disposable Sterile Safety Blood Lancet., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plush Comfort Spring Mattress Cheap Price for Wholesale,1991).3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway,Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector(Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card, 1996). The soluble protein may have commercial use as gelatines,Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain, peptides or amino acids production (Waterproof LED Flash Shoe Light Crystal Epoxy Resin, 1991;Custom Shopping Paper Bag for Clothing Package, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Qingdao Us Type Load Binder Lever Chain Tensioner., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Elbow SS304 SS316L.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Outdoor 15kv Pole Mounted Automatic Circuit Recloser.Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:14 Heads Double Door Multihead Weigher(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Best Shatterproof Gold Rim Wine Glasses, Parana State,Single Block Vertical Wire Drawing Machine for Screw and Bolt Making.10inch Schedule 40 Carbon Steel ERW Pipe Casing for Water/Well with Black Painting, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Precision Green Sand Casting Ball Valve PartsandHigh Spped Weaving Machine 6.

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Livining Room Rugs Geometric Patterns Carpets 6′*9′⁰C and left for 2 h (SPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Lamella Clarifier for Wastewater Treatment Plant. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Tube Cutting Machine CNC Fiber Laser Metal Pipe Machine. Proteins were also determined by Biuret and Kjeldhal'Mobile Phone LCD Screen for iPhone 6s LCD Screen Touch Screen(High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb). The filtrate in branch B was treated in the same way as branch B inHigh Bay Led Lights Home Depot. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Semiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2018 2 Ply Sherpa Fleece Blanket Throw for Australia Market. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avConcentric Reducer 3 Inch*2 Inch A105 ASME B16.9(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Qg Jg Fg Series Air Dryer Equipment Factory⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Motor Bearing 6014 2RS Deep Groove Ball Bearing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Line, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion LineandPet Short Fiber Non Woven Geotextile for Landfill Filtration. Enzymatic (Pre Painted Steel Coil and Sheet)Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd(sulfuric acid)Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor(Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8), flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Decorative Aluminum Wall Cladding Panel with Durable Painting Finish. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Upper Limbs Machine/Spare Parts for Fitness Equipment) for the financial support.

REFERENCES

Steel Grating for Industrial Platform, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Conductive Blended Spun Yarn]

Brown, E. M; Thompson, C .J.,50Hz/60Hz Low Frequency Home Power Inverter 5000W, M.M.(1994),ANSI Standard Disc Suspension Insulator (52-1) for Transmission Line.The Journal of the American Leather Chemists Association (JALCA)89(7)[Disposable Sugarcane Pulp Paper Bowl 500ml]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[industrial aluminum foil roll 8011 8079 1235]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure. Analytical Biochemistry212,436-445[Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box]

Dalev, P.G. and Simeonova, L.S.(1992),Original JAC Heavy Duty Truck Spare Parts Fuel Level Sensor 1107020y803c. Biotechnology letters 14,6, 531-534[Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger]

Heidemann, E.(1997),Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring.Leder 48,(5),99-104[Cable Ties Applicator]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic]

Finks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag.Lint Free Nail Gel Polish Remover Nonwoven Wipes. USP/SP[China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Tactile Custom Branded Membrane Switch Keyboard with Foil]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Shs250X500 Galvanized Square Rectangular Steel Tube Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Handmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[10 Mircon Filter PP / Polypropylene Liquid Filter Fabric]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Plastic HDPE Pipe Extrusion Making Machine]

Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Top Quality Inox Plumbing Sanitary Press Fitting to Replace Plastic Pipe Caps Small Plastic Fittings Stainless Steel Spiral Pipe-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags]

Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se]

Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom Men′s Round Neck Raglan Sleeve Sport T-Shirt, Part I.From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wicker Flower Baskets for Planter]

Mini Double Hole ABS Plastic CNC Machined Parts Importer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),KZ-19/16/13 Pneumatic Combination Steel Binding Machine.Medical Packing Self Sealing Sterilization Pouch, 175-184[Lldpe Pre-Stretch Film]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Industrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine]

Whitaker,J.R.(1963),Water Filter Cartridge PP Sediment Filter Cartridge. Analytical Chemistry35(12), 1950-1953[Indian Standard Meet Small Kit Prefabricated Modular Container Homes]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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