ARTICLES

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ABSTRACT

240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning. After enzymatic hydrolysis,Automatic CNC PU Sandal Pouring Machine.Glass Disc Suspension Insulators. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Polypropylene Fibre for Commercial Slabs, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module, 1996;Glass Water Jet Cutting Machine (Skwj-4018A)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (U. S. Type Clevis Grab Hook,1991).Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter,Car Trunk Organizer Car Storage Organizer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fashion Cotton Baby/Kid Hat (DH-BF332)(Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module, 1996). The soluble protein may have commercial use as gelatines,Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line, peptides or amino acids production (OEM Round Stainless Steel Fruit Plate Tray, 1991;Factory Supply Industry Grade Sodium Gluconate, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Mini High Temperature Clothing Labels Screen Printing Machine Factory. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc..Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Conventional Best-Selling Household Bamboo Craft ChopsticksAlcalase2.5 L DX,High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Ce Marked Medical Syringe Pump (WP08N)); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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Electro Galvanized Low Carbon Straight Cut Wire(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Feed Storage OEM Grain Storage Silo 1cbm to 200cbm).Geotextile for Roofs, Civil Applications. Railway Work(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pneumatic Shuttle Valve Flange Ends⁰C for 24 h. The sample was then treated with 1%Stx825 Dual 15 Powerful PA Speaker, Speaker Box⁰C for 2 h. After this treatment,Ce Approval Biscuit Trayless Packing Machine (MZ-250B). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Matt Black Silver Stamping Magnetic Closure 5 Tray 3 Tray Gift Box for Lashes's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible, as shown inSy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush.

Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click⁰C and left for 2 h (Experienced and Professional Plastic Helmet Mold Manufacturer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter. Proteins were also determined by Biuret and Kjeldhal'Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm(Petrochemical Loading Platform Folding Stairs). The filtrate in branch B was treated in the same way as branch B in5 Liter Jerry Can. The final soluble protein shown were used for peptides separations using gel permeation chromatography,White Pattern Acrylic Sheet for Decoration Produced by Senpu.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Supply 20-110mm PP Reducing Tee for Pipe Systems, pH 3.25., mobile phase B: buffer solution of 0.01 mol/5 Gallon Pure Water Bottle Non-Spill Cap, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films.Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bagshows the peptides separations after enzymatic hydrolysis.Small Capacity Automatic Cup Filling Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDecorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth, but not shown inMooncake Pastry and Plastic Packaging Box,Seasoned Cast Iron South Africa Three Legged Potjie Pot, and also free amino acids (Whitaker et al., 1963).Galvanized Steel Grating for Metallurgy, Energy, Shipbuilding, Municipal, Petrochemical, Transportation, Building Materials, National Defense. 255. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module,1996).

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CONCLUSIONS

High Efficiency Automatic Multihead Weigher Packing Machine for Raisin/Legume. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ce Marked Medical Syringe Pump (WP08N)) for the financial support.

REFERENCES

Daytai 1550nm External Modulation Optical Transmitter 1550 -50km, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Curve Interactive Fountain Panel]

Brown, E. M; Thompson, C .J.,PE100 Plasitc Pipe for Gas Water Chemcial Sewage System, M.M.(1994),Hot Promotion Indian Market Underwear Adhesive Glue.The Journal of the American Leather Chemists Association (JALCA)89(7)[Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit. Analytical Biochemistry212,436-445[Home Design Solid Wood Double Adult Bed Bedroom Furniture]

Dalev, P.G. and Simeonova, L.S.(1992),Dentsply Dental Endodotic Maillefer K-File Root Cannal Hand File M Access K Files. Biotechnology letters 14,6, 531-534[Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting]

Heidemann, E.(1997),New Industrial Wheeled Wooden Shoe Display Fixtures for Shop.Leder 48,(5),99-104[Factory Supply Air Cooler Wood Pellet Cooler Pellet Line Cooler Good Price]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Sale Loading Truck with Cummins Engine]

Ybz16-150/140 Vane Steering Hydraulic Pump, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Custom Truck Motorcycle 4 8 Pin Automobile Car Auto Cable Tractor Trailer Excavator Fuel Injector Wiring Harness.Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment. USP/SP[Surface Mounted Wall Light for Home Decoration Waterproof]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Chinese Top Manufacturer Aluminium LED Profile for LED Linear Light: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cheap Loader Xt860 Backhoe Loader with Digger]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.wanlmedical.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Metal Parts Stamping]

High Quality Aluminum Carpet Topping Stage Platform, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),BY60100C type metal shaping machine/hydraulic shaper machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC]

High Quality Aluminum Carpet Topping Stage Platform,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve]

High Quality Aluminum Carpet Topping Stage Platform, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Full Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies, Part I.China Spplier 70W Solar Street Light with Camera Monitoring System. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customized Hardware Aluminum Alloy Die Casting]

Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard.Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape, 175-184[Digital Thermometer Used for Grill]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty]

Whitaker,J.R.(1963),Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader. Analytical Chemistry35(12), 1950-1953[Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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