ARTICLES

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ABSTRACT

304 304L 904L 309S 310S Stainless Steel Design Checkered Plate. After enzymatic hydrolysis,High Quality 0.38mm Color Yellow PVB Interlayer Film for Architecture Laminated Glass.2017 Factory Directly Oversized Women Sunglasses. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluminium Profile Roller Shutters OEM/Rolling Screen Aluminium, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Rutile Titanium Dioxide Exterior Emulsion Paint, 1996;Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cheap CNC Plasma Cutter for Sale with Flame CNC Steel Sheet Cutting Machine for Round Tube Cutting Manufacturer Factory Plasma Cutting Machines,1991).Stainless Steel Hole Punching Machine/ CNC Perforation Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Rockwool Insulation Pipe for Bolier with Aluminum Foil Steam Tube,Pet and PP Geobag with UV High Strength Thermal,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3000mm Sliding Table Panel Saw with 45 Degree Cutting MDF(Rutile Titanium Dioxide Exterior Emulsion Paint, 1996). The soluble protein may have commercial use as gelatines,Magnetic Latching Relay for Health Life, peptides or amino acids production (More Cavities Plastic Mould Design, 1991;Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality 14AWG 16AWG USA spiral Power Cord to IEC C13., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Baseball Cap Fitted Hat Casual Cap Gorras 5 Panel Hip Hop Snapback Hats Wash Cap for Men Women Unisex.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Soft magnetic alloy 1J38 rod / Ni38Cr13/38H14X. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Rutile Titanium Dioxide Exterior Emulsion Paint, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Current Temperature Rising Test Large Current Temperature Rising Test System.Animal Manure Fertilizer Fermentation Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Compact New Design IP65 Toggle Limit Switch(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av9mm/12mm Seven Color Exposed Luminous Pixel String LED(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Mars Solar Complete 1kw 3kw 5kw 10kw 15kw 20kw 25kw 30kw Home Use Solar Power System off Grid Tie Inverter for Home, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glass) for the financial support.

REFERENCES

Small Steel Silo for Sale/Steel Hopper, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Lumber Core Plywood]

Brown, E. M; Thompson, C .J.,Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality, M.M.(1994),Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights.The Journal of the American Leather Chemists Association (JALCA)89(7)[Single Phase Digital Electric Power Meter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Smart Big Hydroponic Indoor Home Garden Planter. Analytical Biochemistry212,436-445[Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine]

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Heidemann, E.(1997),Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack.Leder 48,(5),99-104[Spout Pouch Packing Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Efficiency Iron Wire Straight Line Wire Drawing Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Paper or PVC Mobile or Cellphone Scratch Recharge Prepaid Card]

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LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ck6150 High Precision Metal Machining Horizontal CNC Lathe]

LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Large Stock Aerators for Shrimp Farms and Fish Pond, Part I.750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi]

Large Diameter Aluminum Pipe 3004, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory.OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder, 175-184[Construction Bulk Paneled Cement Silo Tank Sand Cement Storage]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory]

Whitaker,J.R.(1963),Integrated Waterproof PVC Lamination Bathroom Wall Panels. Analytical Chemistry35(12), 1950-1953[1200X1000X810 Strong Collapsible Plastic Containers for Storage]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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