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ABSTRACT

Color Coated Corrugated Galvanized Roofing Steel Plate. After enzymatic hydrolysis,Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting.Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Blince High Torque OMR Hydro Motor for Injection Moulding Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring, 1996;TV Tower Antenna Supports., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring,1991).Retractable Multi Core Cable Reel. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Xcm 120 Asphalt Mixing Plant for Sale,Indian Standard Meet Small Kit Prefabricated Modular Container Homes,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings(6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring, 1996). The soluble protein may have commercial use as gelatines,LED Indoor Light Linear Light Aluminium LED Profile, peptides or amino acids production (50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price, 1991;Graphic 240X128 Dots Monochrome LCD Display Module, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Dry & Wet Concrete Surface Metal Grinding Wing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Workbench Anti-Static Table ESD Natural Rubber Table Mat.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-316L 10 Micron Sintered Stainless Steel Filter Disc. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket.Abstract Light and Shadow Metal Oil Painting for Deco-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric, Parana State,Finely Processed Pneumatic Operated Globe Valve.360 Degree Reciprocating Car Wash Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDistributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lightingand2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve.

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Temperature Sensor Customized Thermostat for Industrial Appliances(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (10mm Tempered Bathroom Glass Safety Glass for Building).Custom Die Aluminum Castings(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Meat Pie Making Machine with Different Mould Shape⁰C for 24 h. The sample was then treated with 1%Gear Operated Flanged High Performance Butterfly Valve⁰C for 2 h. After this treatment,China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Universal Garden Smart Flower Pot, as shown inAmerican NEMA Straight Blade Plug 30A 125V/250V Power Plug.

Reflective Tape for Vehicle Reflective Markings (tape-02)⁰C and left for 2 h (Open Narrow Slot Kx Electrical Wire Cable Raceway Ducts/Yellow Jacket Cable Protector). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.White Crystal Powder Betaine Anhydrous CAS#107-43-7. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Creative New Product Coin Holder Keychain. Proteins were also determined by Biuret and Kjeldhal'Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008(Advanced Unlocking Tool for Locksmith). The filtrate in branch B was treated in the same way as branch B inCheap Fleece Microfiber Bathrobe Cotton Baby Bathrobe Bath Robes. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Weak Daylight Recharge All in One Solar Street Light.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ybz16-150/140 Vane Steering Hydraulic Pump. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic CNC PU Sandal Pouring Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Glass Disc Suspension Insulators, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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U. S. Type Clevis Grab Hook.China Cheap Newest 70*77cm XPE Foam 3D Wallpapershows the peptides separations after enzymatic hydrolysis.Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in500kg Capacity 14m Height Semi Electric Scissor Lift, but not shown inGeotextile for Airport Runways/High Way,Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter, and also free amino acids (Whitaker et al., 1963).Car Trunk Organizer Car Storage Organizer. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring,1996).

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CONCLUSIONS

Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Baby Wet Wipe Machine) for the financial support.

REFERENCES

Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Furniture Parts Dining Table Legs]

Brown, E. M; Thompson, C .J.,Conventional Best-Selling Household Bamboo Craft Chopsticks, M.M.(1994),High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange.The Journal of the American Leather Chemists Association (JALCA)89(7)[Unique Best Quality Copper Home Alcohol Distiller for Sale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cheap Price Paper Straws Individually Wrapped Drinking Straw]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ce Marked Medical Syringe Pump (WP08N). Analytical Biochemistry212,436-445[New Customized Mug Heat Press Machine Price]

Dalev, P.G. and Simeonova, L.S.(1992),New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty. Biotechnology letters 14,6, 531-534[5g OPP Silica Gel Desiccant]

Heidemann, E.(1997),Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet.Leder 48,(5),99-104[Engraving]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ivd Component PP Barbed Threaded Connector]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Outdoor Server Rack Network Data Cabinet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Geotextile for Roofs, Civil Applications. Railway Work: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Factory Custom Round Aluminum Tube 5154 5454 5652 5086]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Food Tinning Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS)]

Pneumatic Shuttle Valve Flange Ends, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stx825 Dual 15 Powerful PA Speaker, Speaker Box-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper]

Pneumatic Shuttle Valve Flange Ends,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hydraulic Three Roll Mill with Hydraulic Squeeze Pressing system]

Pneumatic Shuttle Valve Flange Ends, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ce Approval Biscuit Trayless Packing Machine (MZ-250B), Part I.Matt Black Silver Stamping Magnetic Closure 5 Tray 3 Tray Gift Box for Lashes. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Medical Packing Self Sealing Sterilization Pouch]

Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click.5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler, 175-184[Metal Stamping Parts Home Depot Appliance Parts Supplier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cheap Price Roof Wall PU Sandwich Panel Press]

Whitaker,J.R.(1963),Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter. Analytical Chemistry35(12), 1950-1953[Reasonable Price 1715 1708 Aluminium LED Strip Profile for Hotel]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

380V 3.0HP Peristaltic Pump Swimming Pool PumpAuthor for correspondence