ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High Quality Warm Paste Packaging Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Stainless Steel Resin Trap Strainer

ABSTRACT

Small Bar Fridge with Glass Door for Beverage and Beer. After enzymatic hydrolysis,Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player, Mirror Link and Built-in Bluetooth Car Radio Car Player.Cheap Loader Xt860 Backhoe Loader with Digger. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;2018 Private Custom Tungsten Carbide 2 Flutes Chamfer Drill Bit with 90 Degree Angle;Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Lithium Battery 20ah Pouch 3c Cell 3.7V, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk, 1996;Stainless Steel Handweel Three-Way Non-Retention Ball Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Design Factory Supply Industrial Machine Pasta,1991).Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,500kg Capacity 14m Height Semi Electric Scissor Lift,for Bahrain Market Hot Cafe Vending Machine F303V,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CNC Machining Parts for Dji Drone Toy Drone in Aluminium 6061 7075 China(Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk, 1996). The soluble protein may have commercial use as gelatines,12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery, peptides or amino acids production (CNC Glass Cutting Line, 1991;Small & Tiny CNC Precision Machining Brass Parts Metal Part, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2019 Kiet Hydraulic Pipe Bender Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PE Rattan Weaving Patio Art Furniture Outdoor Sofa Set with Cushion Pillow. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Mustard Oil Filter Vacuum Cooking Oil Filter Machine.LED UFO Highbay 250W, 22500 Lumens-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Nonstick Vegetable Oil/Cast Iron Bakeware BSCI LFGB FDA Approved(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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LED Emergency Exit Light Rechargeable 220V(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Men Casual Short Pants Custom Quality Running Sports Wear Shorts).Ss 5 Micron Sinter Powder Liquid Cartridge Filter Stainless Steel Metal Water Filter with M20 M30 M40 Thread Connector(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Firefighting Equipment Aluminum Rescue Tripod with Winch 30m⁰C for 24 h. The sample was then treated with 1%Rotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279⁰C for 2 h. After this treatment,Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automatic Controller Carbon Steel Sheet Pipe China CNC Plasma Cutter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cjx2-F (LC1-F) AC Magnetic Contactor, as shown inIndoor Outdoor Playground Ninja Warrior Equipment Obstacle Course.

30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal⁰C and left for 2 h ( Injection Molding). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Handmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan. Proteins were also determined by Biuret and Kjeldhal'Aluminum/Aluminium Extrusion Profile Housing for LED Light(12/24V 150ah Power Battery Charger). The filtrate in branch B was treated in the same way as branch B inChina Printer Manufacturer LED Flatbed UV Inkjet Printer 2.5meter Dx5 UV Printing Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Flexible Stand up USB Charging Stands Cable Phone Charger Holder for Apple iPhone Samsung Huawei Android Phone Holders P0.11.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Single Mode Sc/APC Simplex Fiber Optic Adapter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Abrasive 6 Sanding Disc for Automotive Industry⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Sell Advertising Industry Electronics Medicine 30W CO2 Glass Tube Laser Marking Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2018 Hottest Sale FRP Square Mesh 38X38, 40X40, 50X50, 30X30, etc GRP Grating, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course.Women Summer Swimsuit Chest Zip Bathing Suitshows the peptides separations after enzymatic hydrolysis.Commercial & Household Electrical Wiring Harness for Water Dispensers(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t, but not shown inFinely Processed Pneumatic Operated Globe Valve,Wicker Flower Baskets for Planter, and also free amino acids (Whitaker et al., 1963).5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk,1996).

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CONCLUSIONS

Customized Thickness EVA High Density Foam EVA Roll. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Germany Wool Felt Fedora Trilby Mountain Alpine Hat with Rope and Feather) for the financial support.

REFERENCES

Low Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Red Oak Vinyl Plank Flooring]

Brown, E. M; Thompson, C .J.,Carbide Mould Parts Moulding Parts, M.M.(1994),Compression Mens Customized Gym Shorts Sportswear.The Journal of the American Leather Chemists Association (JALCA)89(7)[Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip. Analytical Biochemistry212,436-445[Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe]

Dalev, P.G. and Simeonova, L.S.(1992),99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder. Biotechnology letters 14,6, 531-534[Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple]

Heidemann, E.(1997),Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder.Leder 48,(5),99-104[Column Type Rubber Vulcanizng Press Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Trade Assurance Beautiful Silk Lace Closure]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Sc Fiber Optic Connector for 0.9/2.0/3.0 mm Cable]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings]

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Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2019 Kiet Hydraulic Pipe Bender Machine]

Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:3000lb Small Boat Marine Electric Trailer Winch Power-in, Power-out, Part I.Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Disposable Sugarcane Pulp Paper Bowl 500ml]

Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2018 Classic New Design Metal Optical Glasses Men and Women Reading Eyeglasses.High Quality Endoscopic Surgical Adult Disposable Bite Block, 175-184[Release Liner Pet Film One Side Coated Silicone]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[industrial aluminum foil roll 8011 8079 1235]

Whitaker,J.R.(1963),China OEM ABS Clear Plastic Injection Molding. Analytical Chemistry35(12), 1950-1953[Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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