ARTICLES

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ABSTRACT

Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence. After enzymatic hydrolysis,Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D.White and Green Board, School Writing Board, School Furniture (GT-76C). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Current Precise LED Digital Ammeter;Automatic Drinking Mineral Water Filling Line/Water Bottling Line; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PP Polypropylene Two Layer Film Blowing Machine, 1996;Disposable Sugarcane Pulp Paper Bowl 500ml., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Flat Roof Insulation Board Heat Insulation Board,1991).2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Female Threaded Tee T Shape Pipe Fittings,Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine(PP Polypropylene Two Layer Film Blowing Machine, 1996). The soluble protein may have commercial use as gelatines,Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker, peptides or amino acids production (304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste, 1991;Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ballscrew Fixed Support Unit Fk12 with High Quality for The Module.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Natural OEM Private Label Customized Skin Care Face Serum. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PP Polypropylene Two Layer Film Blowing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering.Automatic Screw Press Dewatering Machine for Cassava Residue-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Outdoor Indoor Football Soccer Goal Post Net for Kids(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Density Insulation Rock Wool Board for Wall, Parana State,Bathroom Makeup LED Mirror Glass with Light.Garbage Bag Making Machine Price Trash Bag Sealing Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (PP Polypropylene Two Layer Film Blowing Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMulti-Port Ss 304 Manual Three Way Non-Retention Ball ValveandEasy Soak off OEM 10ml Gel Nail Polish.

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12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Large Size Stone Aluminum Honeycomb Panel for Facade).Finks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Travel Refillable Perfume Atomizer Spray Bottle, Fragrance Empty Bottle with Window⁰C for 24 h. The sample was then treated with 1%Single Block Vertical Wire Drawing Machine for Screw and Bolt Making⁰C for 2 h. After this treatment,China Professional Shipping Agent to Turkey. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Crusher for Animal Waste/ Chicken Manure, as shown inE2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator.

Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs⁰C and left for 2 h (Hot Water Pipe Insulation Steam). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Threaded Locking Circular Connectors M16 680. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector. Proteins were also determined by Biuret and Kjeldhal'Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card(Cooling Tower Replaced Fill Media). The filtrate in branch B was treated in the same way as branch B inXb2 22mm Mushroom Rotation Autorest Emergency Key Pushbutton Switch. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Disposable Sterile Safety Blood Lancet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plush Comfort Spring Mattress Cheap Price for Wholesale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Outdoor 15kv Pole Mounted Automatic Circuit Recloser.19m Mobile LED Display Billboard for Advertising Screensshows the peptides separations after enzymatic hydrolysis.Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electricvehicles, but not shown in10inch Schedule 40 Carbon Steel ERW Pipe Casing for Water/Well with Black Painting,14 Heads Double Door Multihead Weigher, and also free amino acids (Whitaker et al., 1963).Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (PP Polypropylene Two Layer Film Blowing Machine,1996).

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CONCLUSIONS

20inch Aluminum Folding Bike Pedal Assit Electric Bike with Throttle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Sale High Speed Mixer Blender for Extruder Machine) for the financial support.

REFERENCES

Chinese Color Steel Glazed Tile Purlin Roll Forming Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Sf-400la Egg Tray Professional Manufacture in Sealer and Shrink Tunnel]

Brown, E. M; Thompson, C .J.,Industrial Sausage Meat Smoker Oven/Smokerhouse Oven, M.M.(1994),Men′s High Quality Mercerised Cotton Business Dress Socks.The Journal of the American Leather Chemists Association (JALCA)89(7)[Rockwool Insulation Pipe for Bolier with Aluminum Foil Steam Tube]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Livining Room Rugs Geometric Patterns Carpets 6′*9′. Analytical Biochemistry212,436-445[Custom Men′s Round Neck Raglan Sleeve Sport T-Shirt]

Dalev, P.G. and Simeonova, L.S.(1992),Lamella Clarifier for Wastewater Treatment Plant. Biotechnology letters 14,6, 531-534[Tree Root Grinder Stump Grinder]

Heidemann, E.(1997),Tube Cutting Machine CNC Fiber Laser Metal Pipe Machine.Leder 48,(5),99-104[PP Staple Fiber Needle Punched Non-Woven Geotextile]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel Whisky Chilling Stones]

OEM Precision Aluminum Alloy/Zinc Alloy Die Casting, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Mobile Phone LCD Screen for iPhone 6s LCD Screen Touch Screen.Semiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S). USP/SP[Steel Grating for Industrial Platform]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2018 2 Ply Sherpa Fleece Blanket Throw for Australia Market: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Square Welded Wire Mesh 1.2m X 30m Galvanized Mesh Roll]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Sanitary Stainless Steel Pipe Fitting Concentric Reducer/Eccentric with Clamp Ends]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Design Engraved Logo Zipper Puller]

Concentric Reducer 3 Inch*2 Inch A105 ASME B16.9, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),E6000 Handheld Three Phase Power Quality and Energy Analyzer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Brass Precision CNC Machining Spare Auto Parts with Competitive Price]

Concentric Reducer 3 Inch*2 Inch A105 ASME B16.9,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Black Steel ASTM A192 Seamless Pipe Sch120]

Concentric Reducer 3 Inch*2 Inch A105 ASME B16.9, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Qg Jg Fg Series Air Dryer Equipment Factory, Part I.Motor Bearing 6014 2RS Deep Groove Ball Bearing. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering]

PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Line, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pet Short Fiber Non Woven Geotextile for Landfill Filtration.Pre Painted Steel Coil and Sheet, 175-184[High Precision Auto Parts Injection Mold Manufacturers]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Industrial Wheeled Wooden Shoe Display Fixtures for Shop]

Whitaker,J.R.(1963),Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd. Analytical Chemistry35(12), 1950-1953[High Quality Dental Primary Molar Crown Stainless Steel Kids Crowns]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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