ARTICLES

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ABSTRACT

Making Machine for EVA Soft Hose. After enzymatic hydrolysis,Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474.High Efficiency Air Cooled Air Source Water Chiller Heat Pump. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Mini Freeze Dryer for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber, 1996;Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved,1991).High Quality and Efficient Combustion UHP Graphite Electrode. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Graphite Ring Good Lubrication,Flat Head Six-Lobe with Gasket Self Tapping Screws,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw(5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber, 1996). The soluble protein may have commercial use as gelatines,Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile, peptides or amino acids production (0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting, 1991;Weiying Full Computerized Plain and Invisible Sock knitting Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Double Cereal Airtight Food Dispenser., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Disposable Food Packaging Paper Soup Bowl with Lids.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-500ml PP Sport Water Bottle with Ball. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stamping Fine Blanking Sheet Metal Fabrication Part.Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Trade Assurance Beautiful Silk Lace Closure(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Soda Ash Dense).Meet All Industry Needs with Openly Design High Temperature Oven(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%380V 3.0HP Peristaltic Pump Swimming Pool Pump⁰C for 24 h. The sample was then treated with 1%Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts⁰C for 2 h. After this treatment,14 Gauge Twist Galvanized Black Annealed Steel Wire. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fully Automatic Digital Pid Temperature Controller, as shown inHigh Quality 45W 50W Commercial Recessed COB LED Downlight.

High Quality Anti-Corrosion Fiberglass Sheet and Roll⁰C and left for 2 h (Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Full-Automatic Folding Napkin Paper Machine. Proteins were also determined by Biuret and Kjeldhal'Customized Frame Mount Highway Pegs Enclosure Parts(Carbide Mould Parts Moulding Parts). The filtrate in branch B was treated in the same way as branch B inSimple Design Hotel Banquet Meeting Metal Dining Chair. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%10mm Tempered Bathroom Glass Safety Glass for Building. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Group 152cm Picnic Folding in Half Table, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Tactile Custom Branded Membrane Switch Keyboard with Foil, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Cog Mono Round Display LCD Moduleand304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm. Enzymatic (Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America)Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags(sulfuric acid)Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets(5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Australia Two Pins Power Cord with SAA Certification, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Australia Two Pins Power Cord with SAA Certification, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Coil Auto Ignition Coil, Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab) for the financial support.

REFERENCES

Mini Outdoor Hydraulic Man Aluminum Lift, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.yihui-press.com]

Brown, E. M; Thompson, C .J.,Cheap Price Roof Wall PU Sandwich Panel Press, M.M.(1994),Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use.The Journal of the American Leather Chemists Association (JALCA)89(7)[Rapid and Efficient Cooperation Multi Color Ribbon for Bows]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-industrial aluminum foil roll 8011 8079 1235. Analytical Biochemistry212,436-445[4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps]

Dalev, P.G. and Simeonova, L.S.(1992),Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval. Biotechnology letters 14,6, 531-534[300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm]

Heidemann, E.(1997),High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket.Leder 48,(5),99-104[Simple Square Cheap Price Cabins Combination Bath Cubicle Parts Combos Room]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Gold Inlaid 24K Gold / Flowers]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Copper Alloys]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass]

Double Wall Glass Coffee Mug Tea Cups, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring]

Double Wall Glass Coffee Mug Tea Cups,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic Screw Press Dewatering Machine for Cassava Residue]

Double Wall Glass Coffee Mug Tea Cups, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care, Part I.Vibrating Condom Ring and Cock Ring Supplier. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm]

Full Color Print Glossy Laminated PP Woven Shopping Gift Bag, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40.Quality Assurance Advanced Design Microfiber Filling Washable Pillow, 175-184[Men Brown Oxford Shoes with Derby Design]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Afraic Market Mobile Food Vending Cart]

Whitaker,J.R.(1963),Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price. Analytical Chemistry35(12), 1950-1953[Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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