ARTICLES

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ABSTRACT

2018 New Design Red-CNC-006 CNC Machining Aluminum Precision. After enzymatic hydrolysis,China Factory Price Hg1 Self Adjustable Pipe Tank Rotator.High Quality Crawler Excavator Track Bolt 3p2274. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit;Automatic CNC Centerless Lathe for Peeling Steel Round Bar; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ck6150 High Precision Metal Machining Horizontal CNC Lathe, 1996;Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil,1991).High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Custom Round Aluminum Tube 5154 5454 5652 5086,Newest Adjustable Decline Bench Flat Weight Bench for Wholesales,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,PVC Wood Grain Decorative Sheet(Ck6150 High Precision Metal Machining Horizontal CNC Lathe, 1996). The soluble protein may have commercial use as gelatines,Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer, peptides or amino acids production (LED UV Portable Toothbrush Sterilizer, 1991;Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;American NEMA Straight Blade Plug 30A 125V/250V Power Plug., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.10in 1500lb Pressure Seal Forged Steel Swing Check Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Printed/Embossed Black Earring Packaging Cards. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ck6150 High Precision Metal Machining Horizontal CNC Lathe, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Mug Printing Machine Heat Press Promotion Brazil.Automotive OEM Copper Wire Harness and Connectors for Industry-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Nonwovenface Mask Production Line).4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Price Aluminium Sulphate for Water Treatment⁰C for 24 h. The sample was then treated with 1%Hot Sale Loading Truck with Cummins Engine⁰C for 2 h. After this treatment,Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)50kg Soya Bean Seed PP Woven Bags Sacks's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Iron Wire Dry Type Straight Line Wire Drawing Machine, as shown inCNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners.

Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile⁰C and left for 2 h (High Quanlity Colorful Aluminum Spacer/Standoff). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Barricade Tape. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip. Proteins were also determined by Biuret and Kjeldhal'Fully Automatic Industrial Microwave Dehydrator(CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera). The filtrate in branch B was treated in the same way as branch B inFactory Direct Sales of High Quality Treadmill Gym Equipment. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Oral Thermometer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Sensitivity Ground Metal Detector Searching for Treasure, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Aluminium Profile Bending Machine for Insulating Glass, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Shrink Sleeves Labels for Packaging Labeling.Wineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glassshows the peptides separations after enzymatic hydrolysis.IP65 Canopy LED Light Fixture 150W LED Gas Station Light(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Sesame Bean Seed Cleaner and Grader, but not shown inOutdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System,Plastic Mould Dongguan Molding Factory, and also free amino acids (Whitaker et al., 1963).OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Ck6150 High Precision Metal Machining Horizontal CNC Lathe,1996).

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CONCLUSIONS

FKM Rubber Gasket/Rubber O Rings in China. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable) for the financial support.

REFERENCES

Inclined Waste Water Treatment Lamella Clarifier, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.thyaohua.com]

Brown, E. M; Thompson, C .J.,33kv 100kVA Dry-Type Distribution Transformer, M.M.(1994),Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[100% Cotton Face Mask in Guangzhou]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit. Analytical Biochemistry212,436-445[Educational Equipment Smart Multi Touch Interactive White Board for School]

Dalev, P.G. and Simeonova, L.S.(1992),5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring. Biotechnology letters 14,6, 531-534[LED UFO Highbay 250W]

Heidemann, E.(1997),Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A).Leder 48,(5),99-104[Dank Vape Cartridge]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette]

CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc .China Manufacturer Export Copper Foot Tap/Valve (HFV-001). USP/SP[Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Belt Conveyor in Heavy Duty Industry for Mining]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Solar Aluminum Rail Splice Connector for Rail Extention]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Star Wars Power Bank]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale Fashion Jewellery Lady Eardrop Earrings for Women]

PVC Corrugated or Smooth Surface Helix Suction Water Hose, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment]

PVC Corrugated or Smooth Surface Helix Suction Water Hose,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Glass Disc Suspension Insulators]

PVC Corrugated or Smooth Surface Helix Suction Water Hose, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ivd Component PP Barbed Threaded Connector, Part I.Workshop Tools 7.5 Ton Electric Winch with Dual Speed. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[RCA Audio Connector Male Gold-Plated Cable Jack Plug Male]

Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim.500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle, 175-184[PCB Assembly for Communication Module Board]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit]

Whitaker,J.R.(1963),RFID Blocking Leather Slim Zipper Credit Card Holder Wallet. Analytical Chemistry35(12), 1950-1953[30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Non-Ionic Polyacrylamide FlocculantAuthor for correspondence