ARTICLES

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ABSTRACT

Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003). After enzymatic hydrolysis,Cheapest Container Living House/Office/Kiosk.Spin Centrifugal Demister Separator or Column Internal. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Baby Wet Wipe Machine;Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (360 Degree Reciprocating Car Wash Machine, 1996;Temperature Sensor Customized Thermostat for Industrial Appliances., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom Die Aluminum Castings,1991).High Quality Meat Pie Making Machine with Different Mould Shape. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Gear Operated Flanged High Performance Butterfly Valve,China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3(360 Degree Reciprocating Car Wash Machine, 1996). The soluble protein may have commercial use as gelatines,Wholesale Universal Garden Smart Flower Pot, peptides or amino acids production (Reflective Tape for Vehicle Reflective Markings (tape-02), 1991;White Crystal Powder Betaine Anhydrous CAS#107-43-7, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Creative New Product Coin Holder Keychain., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Weak Daylight Recharge All in One Solar Street Light. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (360 Degree Reciprocating Car Wash Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ybz16-150/140 Vane Steering Hydraulic Pump.2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:3mm Indoor Wall Cladding Decoration Aluminum Composite Material(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance CleaningAlcalase2.5 L DX,Automatic CNC PU Sandal Pouring Machine, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Glass Disc Suspension Insulators); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic, Parana State,Abstract Light and Shadow Metal Oil Painting for Deco.Polypropylene Fibre for Commercial Slabs, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (360 Degree Reciprocating Car Wash Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the316L 10 Micron Sintered Stainless Steel Filter DiscandCeiling Mounted IV Stand Stainlesss Steel I V Pole.

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Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stainless Steel Resin Trap Strainer).Glass Water Jet Cutting Machine (Skwj-4018A)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%U. S. Type Clevis Grab Hook⁰C for 24 h. The sample was then treated with 1%Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale⁰C for 2 h. After this treatment,Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Car Trunk Organizer Car Storage Organizer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fashion Cotton Baby/Kid Hat (DH-BF332), as shown inESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat.

Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line⁰C and left for 2 h (Drug Delivery ABS Hospital Medical Treatment Medicine Cart). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.OEM Round Stainless Steel Fruit Plate Tray. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Factory Supply Industry Grade Sodium Gluconate. Proteins were also determined by Biuret and Kjeldhal'Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set(Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment). The filtrate in branch B was treated in the same way as branch B inChemical Liquid Coverall Supplier & Factory. The final soluble protein shown were used for peptides separations using gel permeation chromatography,En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Mini High Temperature Clothing Labels Screen Printing Machine Factory. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc.(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Conventional Best-Selling Household Bamboo Craft Chopsticks, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture CabinetandCe Marked Medical Syringe Pump (WP08N). Enzymatic (New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty)Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet(sulfuric acid)CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera(360 Degree Reciprocating Car Wash Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Electro Galvanized Low Carbon Straight Cut Wire, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Electro Galvanized Low Carbon Straight Cut Wire, flow rate of 0.1 mL/min., and l = 620 nm.

Geotextile for Roofs, Civil Applications. Railway Work.High Density Artificial Grass Football Playground Surfaces (STO)shows the peptides separations after enzymatic hydrolysis.Pneumatic Shuttle Valve Flange Ends(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inManufacturer High Strength Carbon Steel Heavy Hex Nut, but not shown inAnti-Pollution Type 160kn Insulator Porclelain Disc,Stx825 Dual 15 Powerful PA Speaker, Speaker Box, and also free amino acids (Whitaker et al., 1963).Ce Approval Biscuit Trayless Packing Machine (MZ-250B). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (360 Degree Reciprocating Car Wash Machine,1996).

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CONCLUSIONS

SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Glass Disc Suspension Insulators) for the financial support.

REFERENCES

Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2 Ton High Quality Electric Chain Block with SGS]

Brown, E. M; Thompson, C .J.,110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator, M.M.(1994),Supply 20-110mm PP Reducing Tee for Pipe Systems.The Journal of the American Leather Chemists Association (JALCA)89(7)[Best Selling Wooden Grain Metal Aluminium Fence Panels for Sale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[CS1363 a Standard UK 3 Pin Power Plug]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-5 Gallon Pure Water Bottle Non-Spill Cap. Analytical Biochemistry212,436-445[3DTALK FAB460 Large Printing Size 420*420*460mm Industrial Level FDM 3D Printer with Multiple Consumables]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks. Biotechnology letters 14,6, 531-534[on-Sale Flat Transparent Glass Jar for Sweet]

Heidemann, E.(1997),7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care.Leder 48,(5),99-104[Ammonia Ice Evaporator]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector]

316L 10 Micron Sintered Stainless Steel Filter Disc, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Solid Carbide Single Flute End Mills Oblique Angle Cutting.High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies. USP/SP[T Slot Aluminum Extrusion Ob4040b Aluminium Profile]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Power Plant Circulating Water Pump & Spare Part]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Desktop Fiber Laser Marking Machines]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Chenille Microfiber Mop Head]

400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Small Capacity Automatic Cup Filling Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine]

400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Luxury Design French Style MDF/HDF Molded Wood Door Skin Solid Wooden 2 Panel Wood Door]

400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Seasoned Cast Iron South Africa Three Legged Potjie Pot, Part I.Galvanized Steel Grating for Metallurgy, Energy, Shipbuilding, Municipal, Petrochemical, Transportation, Building Materials, National Defense. 255. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Solid Carbide Single Flute End Mills Oblique Angle Cutting]

Plastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2019 New Release Doypack Sachet Powder Packing Machine Price.Amplifier Tube Glass Vacuum Tube (833C), 175-184[Ball Nose Router Bit Factory OEM/ODM Products]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[OEM Box Sealing High Bonding Crystal Super Clear BOPP Packing Tape]

Whitaker,J.R.(1963),Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools. Analytical Chemistry35(12), 1950-1953[2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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