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ABSTRACT

Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch. After enzymatic hydrolysis,Customized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage.Factory Cheap Price Wholesale Black Nickel Bag O Ring Metal, Professional Custom 43mm Metal O-Ring for Bag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11;High Quality Sesame Bean Seed Cleaner and Grader; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pet Strapping Band Production Line, 1996;UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag, RFID Paper Label, Smart Label., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Customized Specification Mesh Water Filter SS316 Filters for Liquids Filtration,1991).PA Flexible Plastic Corrugated Loom Tubing. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl,Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet(Pet Strapping Band Production Line, 1996). The soluble protein may have commercial use as gelatines,3-Way T Type Elbow Quick Connect Pneumatic Fitting, peptides or amino acids production (China Superior Quality Soya Protein Food Production Machine Line, 1991;Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom Army Style 100% Polyester Fiber Mens Polo Shirt., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Twisted Annealed Wire for Binding. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pet Strapping Band Production Line, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid.Wholesale Fashion Jewellery Lady Eardrop Earrings for Women-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service, Parana State,Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring.High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Pet Strapping Band Production Line, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the19m Mobile LED Display Billboard for Advertising ScreensandWallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electricvehicles.

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Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (AISI 304 / 316 Stainless Steel Braided PTFE Hose Pipe).High Quality Low Volume Production Aluminum CNC Machining Auto Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple⁰C for 24 h. The sample was then treated with 1%New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank⁰C for 2 h. After this treatment,Carbon Steel Round/Square Pipe Tube Production Line. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Green PVC Coated Front Yard Double Wire Mesh Fence Panel's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cable Length 11 Gun Safety Padlock, as shown inOutdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light.

Canvas School College Backpack/Bookbags for Girls/Students/Women⁰C and left for 2 h (Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Women′s Seamless Strapless Bra. Proteins were also determined by Biuret and Kjeldhal'Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet(Lf + Hf Contactless Smart Hybrid Card). The filtrate in branch B was treated in the same way as branch B inwww.jprmodel.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest.Small Portable Scooters Folding Electric Scooters for Adultsshows the peptides separations after enzymatic hydrolysis.Ride on Toys Riding Animals in The Mall(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Selling House Floor Cleaning 360 Spin Mop, but not shown inUnderground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket,Finely Processed Pneumatic Operated Globe Valve, and also free amino acids (Whitaker et al., 1963).Color Coated Corrugated Galvanized Roofing Steel Plate. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Pet Strapping Band Production Line,1996).

99% L-Tryptophan

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Customized Stainless Steel Door Lock Strike Plate Metal Lock BracketandCustom Men′s Round Neck Raglan Sleeve Sport T-Shirtshow the gel chromatographic separation of soluble protein with Sephadex G-Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting.Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms, and another peak at 45 mL not shown inTree Root Grinder Stump Grinder, for peptides lower than 3000 Da.Precision Diamond Wire Saw Rope for Cutting Concrete Stone(Condell et al.,1993) .

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Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty, V_e, and K_avcalculated as discussed previously.Blince High Torque OMR Hydro Motor for Injection Moulding Machine,6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Steel Grating for Industrial Platform) (Reis et al.,1989;China Superior Quality Soya Protein Food Production Machine Line.,1991;UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag, RFID Paper Label, Smart Label.,1991).TV Tower Antenna Supports(1992), were lower, except for cystine and tyrosine,Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring.

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CONCLUSIONS

Retractable Multi Core Cable Reel. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stainless Steel CF8/CF8m Street Elbow Pipe Fittings) for the financial support.

REFERENCES

New Xcm 120 Asphalt Mixing Plant for Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fashion T Bar Shoes Soft Leather Baby]

Brown, E. M; Thompson, C .J.,Indian Standard Meet Small Kit Prefabricated Modular Container Homes, M.M.(1994),Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Design Engraved Logo Zipper Puller]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Brass Precision CNC Machining Spare Auto Parts with Competitive Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-LED Indoor Light Linear Light Aluminium LED Profile. Analytical Biochemistry212,436-445[Black Steel ASTM A192 Seamless Pipe Sch120]

Dalev, P.G. and Simeonova, L.S.(1992),50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price. Biotechnology letters 14,6, 531-534[Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering]

Heidemann, E.(1997),Graphic 240X128 Dots Monochrome LCD Display Module.Leder 48,(5),99-104[Oil Pan For Jeep Compass]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Industrial Wheeled Wooden Shoe Display Fixtures for Shop]

Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Dry & Wet Concrete Surface Metal Grinding Wing.Workbench Anti-Static Table ESD Natural Rubber Table Mat. USP/SP[High Quality Dental Primary Molar Crown Stainless Steel Kids Crowns]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Compact New Design IP65 Toggle Limit Switch]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-316L 10 Micron Sintered Stainless Steel Filter Disc: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PE Film Blowing Machine (SJ-1000-1300-1500)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Compounding Pellet Glass Fiber Extruder]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Carbon Steel Equal Tee for Connection of Pipe]

Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Abstract Light and Shadow Metal Oil Painting for Deco-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Rutile Titanium Dioxide Exterior Emulsion Paint]

Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Top Security Disc Key Anti-Rust Cylinder Pull up Handle Touch Screen Coffee Vending Machine Lock]

Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003), Part I.Cheapest Container Living House/Office/Kiosk. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stainless Steel Hole Punching Machine/ CNC Perforation Machine]

Spin Centrifugal Demister Separator or Column Internal, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Baby Wet Wipe Machine.Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump, 175-184[China 330 W Solar Panel]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Max Twintier Rb441t Using Wire Coils Tw1061t]

Whitaker,J.R.(1963),Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric. Analytical Chemistry35(12), 1950-1953[China Factory Offer New Core I7 15.6 Laptop]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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