ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Tree Root Grinder Stump Grinder. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Open Narrow Slot Kx Electrical Wire Cable Raceway Ducts/Yellow Jacket Cable Protector

ABSTRACT

Customization! Bdy380CD Horizontal Directional Drilling Machine. After enzymatic hydrolysis,V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine.Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Programmable Salt Spray Test Chamber for Corrosion Testing/Test, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Professional Manufacturer Pure Wolfram Wire with High Purity 99.95%, 1996;FKM Bearing Oil Seal /Customized., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Selling Shandong Manufacturer 3 4 5 Inch Rubber Sealing Ring Joint UPVC Rrj Pipe for Water Supply and Sewage/Drainage 8kg Rate PVC Tubes,1991).T Slot Aluminum Extrusion Ob4040b Aluminium Profile, Industrial Aluminum Profile. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce,Max Twintier Rb441t Using Wire Coils Tw1061t,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm(Professional Manufacturer Pure Wolfram Wire with High Purity 99.95%, 1996). The soluble protein may have commercial use as gelatines,Manufacturer Supply Durable Grey Color Non-Slip EVA Mat, peptides or amino acids production (Feed Storage OEM Grain Storage Silo 1cbm to 200cbm, 1991;Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Captain Woodwork Sanding Machine/Sanding Table., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Atg39 100W LED Flood Light Wall Light Wall Washer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Professional Manufacturer Pure Wolfram Wire with High Purity 99.95%, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net.Gold Inlaid 24K Gold / Flowers-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Ml33-2mm Eddy Current Linear Position/Displacement Sensor⁰C and left for 2 h (High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Veterinarian Ultrasound Diagnostic System for Cow, Horse, Swine, Goat. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain. Proteins were also determined by Biuret and Kjeldhal'Wholesale Long Lasting Custom Private Label Glitter Lip Gloss(Indoor Inkjet Media Material). The filtrate in branch B was treated in the same way as branch B inGlassware/200ml Dark Green Red Wine Bottle. The final soluble protein shown were used for peptides separations using gel permeation chromatography,ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Various Bridge NdFeB Motor Magnets with High Performance, pH 3.25., mobile phase B: buffer solution of 0.01 mol/White Melamine Powder for Industrial Uses, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

3000mm Sliding Table Panel Saw with 45 Degree Cutting MDF. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hydraulic Cutter Bread Machine (ZMF-20Y)) for the financial support.

REFERENCES

Magnetic Latching Relay for Health Life, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Automatic Aerosol Spray Tin Canning Production Line]

Brown, E. M; Thompson, C .J.,More Cavities Plastic Mould Design, M.M.(1994),Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300.The Journal of the American Leather Chemists Association (JALCA)89(7)[Egg 0b 309 Fixed Panel Socket [Push Pull Circular Connector]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality 14AWG 16AWG USA spiral Power Cord to IEC C13. Analytical Biochemistry212,436-445[Plastic Grip Wood Handle Shovel]

Dalev, P.G. and Simeonova, L.S.(1992),Baseball Cap Fitted Hat Casual Cap Gorras 5 Panel Hip Hop Snapback Hats Wash Cap for Men Women Unisex. Biotechnology letters 14,6, 531-534[22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine]

Heidemann, E.(1997),Soft magnetic alloy 1J38 rod / Ni38Cr13/38H14X.Leder 48,(5),99-104[1/2-4 Inch Red Handle Pressure PVC White Ball Valve]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[PE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Compact New Design IP65 Toggle Limit Switch: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ck6150 High Precision Metal Machining Horizontal CNC Lathe]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hydraulic Hose SAE 100 R1AT]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi]

Teflon for Industrial Plastic Gasket Seal with Good Quality, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Double Walled Stainless Steel Insulated Water Bottle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory]

Teflon for Industrial Plastic Gasket Seal with Good Quality,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[1200X1000X810 Strong Collapsible Plastic Containers for Storage]

Teflon for Industrial Plastic Gasket Seal with Good Quality, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glass, Part I.2018 Cheap PU Leather Bracelet in Alloy Jewelry. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2019 New Advertising Inflatable LED Jellyfish Balloon for Sale]

China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500.Competetive Price Tension Springs for Stapler, 175-184[Supplier LED Headlight LED Work Light Dt Connector Jeep Offroad Truck LED Light Bar Automotive Wiring Harness]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Power Tools Lithium Battery Cordless Drill (GBK2-66108TS)]

Whitaker,J.R.(1963),Aramid Felt Teflon Felt Non-Woven Nomex Needle Punched Machine/ Felt Polishing Wheel Polished Felt CNC Digital Cutter Textile Felt Making Machine. Analytical Chemistry35(12), 1950-1953[China Manufacturer Garbage Incinerator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

OEM SS304 Stainless Steel Sw Reducing Tee for Food MachineryAuthor for correspondence