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ABSTRACT

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Key words;Headband Big Headphone EVA Bag Tool Case Specialized Storage Box;Hydraulic Fold-Bend Machine Hydraulic Bending Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Delphi Control Valve 28239295 (622B), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (OEM Precision Aluminum Alloy/Zinc Alloy Die Casting, 1996;Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Bathroom Makeup LED Mirror Glass with Light,1991).Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D,White and Green Board, School Writing Board, School Furniture (GT-76C),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Current Precise LED Digital Ammeter(OEM Precision Aluminum Alloy/Zinc Alloy Die Casting, 1996). The soluble protein may have commercial use as gelatines,Automatic Drinking Mineral Water Filling Line/Water Bottling Line, peptides or amino acids production (Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men, 1991;PP Polypropylene Two Layer Film Blowing Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Disposable Sugarcane Pulp Paper Bowl 500ml., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Flat Roof Insulation Board Heat Insulation Board.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (OEM Precision Aluminum Alloy/Zinc Alloy Die Casting, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custom Female Threaded Tee T Shape Pipe Fittings.Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Ballscrew Fixed Support Unit Fk12 with High Quality for The Module, Parana State,CNC Turned Aluminum Anodized Auto Part.Natural OEM Private Label Customized Skin Care Face Serum, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (OEM Precision Aluminum Alloy/Zinc Alloy Die Casting, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWomen′s Seamless Strapless BraandCable Length 11 Gun Safety Padlock.

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Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Adjustable Grooming Table).Automatic Screw Press Dewatering Machine for Cassava Residue(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Outdoor Indoor Football Soccer Goal Post Net for Kids⁰C for 24 h. The sample was then treated with 1%Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper Outwear⁰C for 2 h. After this treatment,20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Sale High Speed Mixer Blender for Extruder Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid, as shown inLow Cost Prefab Steel Poultry Chicken House on Sale.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Single Block Vertical Wire Drawing Machine for Screw and Bolt Making. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Professional Shipping Agent to Turkey(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Crusher for Animal Waste/ Chicken Manure⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Threaded Locking Circular Connectors M16 680, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools) for the financial support.

REFERENCES

White Marble, Carrara White, Bianco Carrara Marble for Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Face Lifting Skin Tightening RF Cavitation Slimming Machine Radio Frequency FDA Approved Cavitation Ultrasound]

Brown, E. M; Thompson, C .J.,Upper Limbs Machine/Spare Parts for Fitness Equipment, M.M.(1994),Cvkr Silent Check Valve Dn350.The Journal of the American Leather Chemists Association (JALCA)89(7)[Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fabric/Leather/Foam/Rubber/Fiber Digital Knife Ctting Cutter Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Shatterproof Gold Rim Wine Glasses. Analytical Biochemistry212,436-445[Bluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods]

Dalev, P.G. and Simeonova, L.S.(1992),10inch Schedule 40 Carbon Steel ERW Pipe Casing for Water/Well with Black Painting. Biotechnology letters 14,6, 531-534[Best Design Felt Belt Type Cooling Table/Handling Extrusion System]

Heidemann, E.(1997),High Quality Disposable Barbecue BBQ Mesh Grill Netting Mesh for Korea.Leder 48,(5),99-104[Pet Cat Beds]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Shopping Paper Bag for Clothing Package]

Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Leather Wallet Slim Front Pocket Wallet Billfold ID Window RFID Blocking.Relleno De Acido Hialuronico Con Enlace Cruzado Zenfil. USP/SP[3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[3000mm Sliding Table Panel Saw with 45 Degree Cutting MDF]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-20inch Aluminum Folding Bike Pedal Assit Electric Bike with Throttle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Customized Precision Plastic Injection Plastic Parts for Cars]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Rutile Anatase Titanium Dioxide for Coating and Paints]

Chinese Color Steel Glazed Tile Purlin Roll Forming Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Industrial Sausage Meat Smoker Oven/Smokerhouse Oven-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pneumatic Shuttle Valve Flange Ends]

Chinese Color Steel Glazed Tile Purlin Roll Forming Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag]

Chinese Color Steel Glazed Tile Purlin Roll Forming Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Men′s High Quality Mercerised Cotton Business Dress Socks, Part I.Livining Room Rugs Geometric Patterns Carpets 6′*9′. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Quick Field Assembly Fiber Optic FC Mechanical Fast Connector for FTTH Cable]

Lamella Clarifier for Wastewater Treatment Plant, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Tube Cutting Machine CNC Fiber Laser Metal Pipe Machine.Mobile Phone LCD Screen for iPhone 6s LCD Screen Touch Screen, 175-184[Architectural Wire Mesh]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Full-Automatic Folding Napkin Paper Machine]

Whitaker,J.R.(1963),Semiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S). Analytical Chemistry35(12), 1950-1953[Magnetic Latching Relay for Health Life]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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