ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera

ABSTRACT

Electro Galvanized Low Carbon Straight Cut Wire. After enzymatic hydrolysis,Geotextile for Roofs, Civil Applications. Railway Work.Pneumatic Shuttle Valve Flange Ends. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Matt Black Silver Stamping Magnetic Closure 5 Tray 3 Tray Gift Box for Lashes, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible, 1996;Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler,1991).Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm,White Pattern Acrylic Sheet for Decoration Produced by Senpu,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16(Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible, 1996). The soluble protein may have commercial use as gelatines,SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends, peptides or amino acids production (Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production, 1991;110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Supply 20-110mm PP Reducing Tee for Pipe Systems., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.5 Gallon Pure Water Bottle Non-Spill Cap.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care.Solid Carbide Single Flute End Mills Oblique Angle Cutting-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Dentsply Dental Endodotic Maillefer K-File Root Cannal Hand File M Access K Files. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Industrial Wheeled Wooden Shoe Display Fixtures for Shop(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Chinese Top Manufacturer Aluminium LED Profile for LED Linear Light, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Aluminum Carpet Topping Stage Platform, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Small Capacity Automatic Cup Filling Machine) for the financial support.

REFERENCES

Petrochemical Loading Platform Folding Stairs, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Powerful High Temperature SmCo Magnet Arc Magnet]

Brown, E. M; Thompson, C .J.,64mm Double Braid UHMWPE Tugboat Mooring Rope, M.M.(1994),Rubber Soles Vulcanizing Machine/ Press Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Mini Double Hole ABS Plastic CNC Machined Parts Importer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[12oz 16oz Glass Mason Jar with Handle and Straw]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable. Analytical Biochemistry212,436-445[Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Sell Electro Hydraulic Synchronous CNC Press Brake Kcn-30032. Biotechnology letters 14,6, 531-534[Custom Printed/Embossed Black Earring Packaging Cards]

Heidemann, E.(1997),AISI 304 / 316 Stainless Steel Braided PTFE Hose Pipe.Leder 48,(5),99-104[m.szfanshitong.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Underground Carport]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Commecial Use Watch Display Cabinet for Watch Store Interior Design]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Forged Steel Pressure Sealed Bonnet Globe Valve: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ce Marked Medical Syringe Pump (WP08N)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cheap Fireplace Tv Stand Wholesaler Quotes & PriceList]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD]

Stable Adjustable Monitor Mounts Folding LCD Monitor Stand, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System]

Stable Adjustable Monitor Mounts Folding LCD Monitor Stand,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Aluminum Sheet Metal Case Parts for Industrial Computer]

Stable Adjustable Monitor Mounts Folding LCD Monitor Stand, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush, Part I.Manual Double Bridge Glass Cutting Table Machine (JFQG-2620). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile]

2018 New Design Red-CNC-006 CNC Machining Aluminum Precision, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Factory Price Hg1 Self Adjustable Pipe Tank Rotator.High Quality Crawler Excavator Track Bolt 3p2274, 175-184[m.qhmixdrymortarplant.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wall Mounting Metal Control Box Control Desk]

Whitaker,J.R.(1963),SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit. Analytical Chemistry35(12), 1950-1953[Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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