ARTICLES

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ABSTRACT

New Design Factory Supply Industrial Machine Pasta. After enzymatic hydrolysis,Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager.500kg Capacity 14m Height Semi Electric Scissor Lift. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CNC Glass Cutting Line, 1996;Small & Tiny CNC Precision Machining Brass Parts Metal Part., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera,1991).2019 Kiet Hydraulic Pipe Bender Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PE Rattan Weaving Patio Art Furniture Outdoor Sofa Set with Cushion Pillow,Stainless Steel Mustard Oil Filter Vacuum Cooking Oil Filter Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,LED UFO Highbay 250W, 22500 Lumens(CNC Glass Cutting Line, 1996). The soluble protein may have commercial use as gelatines,Nonstick Vegetable Oil/Cast Iron Bakeware BSCI LFGB FDA Approved, peptides or amino acids production (Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine, 1991;150*100mm Rearview Mirror Anti Fog Film Rain Proof Oval Film for SUV Car Mirror, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Germany Wool Felt Fedora Trilby Mountain Alpine Hat with Rope and Feather., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cleaning Lint Free Microfiber Mop.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-L2.072.324 Pm74 Sm74 XL75 Hdm Bellows Feeder Dust Cover Length 80mm. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CNC Glass Cutting Line, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine.LED Emergency Exit Light Rechargeable 220V-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ss 5 Micron Sinter Powder Liquid Cartridge Filter Stainless Steel Metal Water Filter with M20 M30 M40 Thread Connector(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Handmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board).Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Aluminum/Aluminium Extrusion Profile Housing for LED Light⁰C for 24 h. The sample was then treated with 1%Flexible Stand up USB Charging Stands Cable Phone Charger Holder for Apple iPhone Samsung Huawei Android Phone Holders P0.11⁰C for 2 h. After this treatment,Single Mode Sc/APC Simplex Fiber Optic Adapter. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine, as shown inOriginal JAC Heavy Duty Truck Spare Parts Fuel Level Sensor 1107020y803c.

Abrasive 6 Sanding Disc for Automotive Industry⁰C and left for 2 h (Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Sell Advertising Industry Electronics Medicine 30W CO2 Glass Tube Laser Marking Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,2018 Hottest Sale FRP Square Mesh 38X38, 40X40, 50X50, 30X30, etc GRP Grating. Proteins were also determined by Biuret and Kjeldhal'Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm(Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights). The filtrate in branch B was treated in the same way as branch B inBathroom Vanity Sets Manufacturers Cheap Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Single Sphere Rubber Expansion Joint.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cold Press Circular Wood Cutting Machine Blade. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGalvanized Trench Cover(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Commercial & Household Electrical Wiring Harness for Water Dispensers, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wicker Flower Baskets for Planter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Sofiderm Best Selling Breast Enhancer Injectable Hyaluronic Acid Dermal Filler with Ce.New Fashion Diaper Bags Mummy Bagshows the peptides separations after enzymatic hydrolysis.Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike, but not shown inClassic Black Color Commercial Glass Door Multi Deck Refrigerator,Fiber Laser Marking Machine for Aluminum Alloy, Copper, Iron, Metal Knife, and also free amino acids (Whitaker et al., 1963).Refillable Airless Cosmetic Packaging 10ml 15ml 20ml 30ml Serum Bottle. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (CNC Glass Cutting Line,1996).

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Compression Mens Customized Gym Shorts Sportswear, V_e, and K_avcalculated as discussed previously.Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip,99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Bonfiglioli Ta Series Helical Gearing Gearbox) (Reis et al.,1989;Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine.,1991;Small & Tiny CNC Precision Machining Brass Parts Metal Part.,1991).Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder(1992), were lower, except for cystine and tyrosine,P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display.

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CONCLUSIONS

Electric Furniture Lift. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer) for the financial support.

REFERENCES

Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Massage Chair Replacement Seats]

Brown, E. M; Thompson, C .J.,Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet, M.M.(1994),Construction Material Hoist Rack and Pinion.The Journal of the American Leather Chemists Association (JALCA)89(7)[Supply 20-110mm PP Reducing Tee for Pipe Systems]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Slide Switch - DC Power Jack RoHS]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-3000lb Small Boat Marine Electric Trailer Winch Power-in, Power-out. Analytical Biochemistry212,436-445[Cheap Bedroom Furniture Popular Wardrobe Set (CAS-BD1808)]

Dalev, P.G. and Simeonova, L.S.(1992),Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks. Biotechnology letters 14,6, 531-534[Tractor Tine Tiller Cultivator]

Heidemann, E.(1997),Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal.Leder 48,(5),99-104[Auto Parts Supplier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Car Trunk Organizer Car Storage Organizer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China OEM ABS Clear Plastic Injection Molding: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Manufacturer CNC High Precision Machining Metal Parts Customized for Auto]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Professional Leather Portfolio]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Small Capacity Automatic Cup Filling Machine]

Widely Use 32 Cavities Pet Preform Water Bottle Mould, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Black Powder Coated Steel Sheet Metal Housing-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China High Efficiency Automatic Glue Dispensing Machine with Factory Price]

Widely Use 32 Cavities Pet Preform Water Bottle Mould,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning]

Widely Use 32 Cavities Pet Preform Water Bottle Mould, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Alloy Steel Hot Forged 4140 42CrMo4 4340 Forging Ring, Part I.Hot Sell 100% Seal Hexagon Glass Jam Jar. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont]

Metal Parts Stamping, Stamped Brass Shrapnel,   Brass Connect Stamping, Electronic Connection Piece Metal Stamped Part, Copper, Brass Connect Stamping, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair.Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper Tape, 175-184[Factory Backpack 4 in 1 Garden Cutting Tool Knapsack Brush Cutter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring]

Whitaker,J.R.(1963),Heavy Caliber Copper Rectangular Steel Tube. Analytical Chemistry35(12), 1950-1953[High Quality Aluminum Carpet Topping Stage Platform]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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