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ABSTRACT

5 Gallon Pure Water Bottle Non-Spill Cap. After enzymatic hydrolysis,Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks.7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films, 1996;Small Capacity Automatic Cup Filling Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Seasoned Cast Iron South Africa Three Legged Potjie Pot,1991).Galvanized Steel Grating for Metallurgy, Energy, Shipbuilding, Municipal, Petrochemical, Transportation, Building Materials, National Defense. 255. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machine,2019 New Release Doypack Sachet Powder Packing Machine Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Amplifier Tube Glass Vacuum Tube (833C)(400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films, 1996). The soluble protein may have commercial use as gelatines,Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools, peptides or amino acids production (New Style Custom Logo Makeup Multi Colored Eyeshadow Palette, 1991;Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;20cm Titanium Aluminum Stainless Steel Non Stick Fry Pan on Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Efficiency Automatic Multihead Weigher Packing Machine for Raisin/Legume. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Daytai 1550nm External Modulation Optical Transmitter 1550 -50km.PE100 Plasitc Pipe for Gas Water Chemcial Sewage System-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Promotion Indian Market Underwear Adhesive Glue(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Quality Aluminum Carpet Topping Stage Platform(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Nice Quality Coloring Printing Children Board Book Printing).BY60100C type metal shaping machine/hydraulic shaper machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Full Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies⁰C for 24 h. The sample was then treated with 1%China Spplier 70W Solar Street Light with Camera Monitoring System⁰C for 2 h. After this treatment,Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape, as shown inTumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling.

Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader⁰C and left for 2 h (Mini Double Hole ABS Plastic CNC Machined Parts Importer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory. Proteins were also determined by Biuret and Kjeldhal'Masturbators Adult Sex Toys Japanese Love Doll(12oz 16oz Glass Mason Jar with Handle and Straw). The filtrate in branch B was treated in the same way as branch B inneodermalfiller. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Cheap 20tons LPG Semi Trailers Gas Tank Transport Trailer for Sale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Carbon Steel Spherical Plain Radical Bearings. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avElectrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.IR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipment⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Powder Spray Booth for Metal Furniture, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Small Tin Box for Condoms, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with CanopyandDrive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price. Enzymatic (Petrochemical Loading Platform Folding Stairs)64mm Double Braid UHMWPE Tugboat Mooring Rope(sulfuric acid)Rubber Soles Vulcanizing Machine/ Press Machine(400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Industrial Wheeled Wooden Shoe Display Fixtures for Shop) for the financial support.

REFERENCES

Automatic CNC Centerless Lathe for Peeling Steel Round Bar, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Electronics Metal Stamping]

Brown, E. M; Thompson, C .J.,High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence, M.M.(1994),Ck6150 High Precision Metal Machining Horizontal CNC Lathe.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ultra-Wide Nonmagnetic 5052 Aluminum Alloy Coil for Kitchen Cabinets]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Semiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting. Analytical Biochemistry212,436-445[Etched Stainless Steel Titanium Sheet]

Dalev, P.G. and Simeonova, L.S.(1992),Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil. Biotechnology letters 14,6, 531-534[CNC Turned Aluminum Anodized Auto Part]

Heidemann, E.(1997),High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter.Leder 48,(5),99-104[Smd Led Advertising Screen]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ml33-2mm Eddy Current Linear Position/Displacement Sensor]

Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Factory Custom Round Aluminum Tube 5154 5454 5652 5086.Newest Adjustable Decline Bench Flat Weight Bench for Wholesales. USP/SP[Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-PVC Wood Grain Decorative Sheet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Waterproof BNC Connector for Industry]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cartoon Printed Baby Boys Short Sleeve T Shirt for Summer Infant Kids Cotton T-Shirts Clothes Toddler Letter Tops]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm]

Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),LED UV Portable Toothbrush Sterilizer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Self-Propelled Wheel Farm Using 3 Rows Corn Harvester]

Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stamping Fine Blanking Sheet Metal Fabrication Part]

Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding, Part I.American NEMA Straight Blade Plug 30A 125V/250V Power Plug. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Weiying Full Computerized Plain and Invisible Sock knitting Machine]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts]

Whitaker,J.R.(1963),Automotive OEM Copper Wire Harness and Connectors for Industry. Analytical Chemistry35(12), 1950-1953[Excellent Plastic Extruder in Pet PP Packing Strap Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

for Granule and Powder Selection and ClassificationAuthor for correspondence