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ABSTRACT

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Key words;Wire Mesh Welding Size 10mm X 10mm;P4.75 Indoor Single Dual Color Advertising LED Display Billboard; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plug-in Assembly Type Straight Through Joint and Insulation Joint, 1996;Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin,1991).Quilting Machine for Muti- Needle Mattress Quilting Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Single-Screw Plastic Extruders Machine,Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quanlity Colorful Aluminum Spacer/Standoff(Plug-in Assembly Type Straight Through Joint and Insulation Joint, 1996). The soluble protein may have commercial use as gelatines,5g OPP Silica Gel Desiccant, peptides or amino acids production (Electric Winch With Groove Drum, 1991;Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Truck Air Dryer cartridge 14bar., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3mm White Waterproof PVC Foam Board. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plug-in Assembly Type Straight Through Joint and Insulation Joint, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Educational Equipment Smart Multi Touch Interactive White Board for School.Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Women Party Jewelry 45cm Sequins Choker(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag).Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Comfort Furniture Natural Spring Memory Foam Mattress⁰C for 24 h. The sample was then treated with 1%Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume⁰C for 2 h. After this treatment,4.2A Type a Type C Pd QC3.0 USB Charger. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ferrous Sulphate Monohydrate 30%Min Factory Wholesale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Small Portable Scooters Folding Electric Scooters for Adults, as shown in2194230012 for Mercedes Benz Auto Spare Parts Brake Disc.

Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant⁰C and left for 2 h (Reverse Flow Configuration Axial Fan). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle. Proteins were also determined by Biuret and Kjeldhal'Most Popular Split Pressurized Flat Panel Solar Water Heater(Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet). The filtrate in branch B was treated in the same way as branch B inDriving Shaft. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustomized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947, pH 3.25., mobile phase B: buffer solution of 0.01 mol/RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Good Quality Banded Type TeeandPrepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles. Enzymatic (Cog Mono Round Display LCD Module)4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black(sulfuric acid)460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric(Plug-in Assembly Type Straight Through Joint and Insulation Joint, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%China Products Suppliers Camlock Coupling Quick Couplings Female, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%China Products Suppliers Camlock Coupling Quick Couplings Female, flow rate of 0.1 mL/min., and l = 620 nm.

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Fireproof Aluminum Curtain Wall Panel, V_e, and K_avcalculated as discussed previously.Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple,High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Besen Portable EV Fast Charger  with Schuko Plug) (Reis et al.,1989;Electric Winch With Groove Drum.,1991;Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235).,1991).China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board(1992), were lower, except for cystine and tyrosine,Adjustable Grooming Table.

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CONCLUSIONS

High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Freqency PCB with Blind Via) for the financial support.

REFERENCES

PE Coated Paper for Salt Sugar Sachets Paper Food Grade, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Metal Processing Machinery Parts/Sheet Metal Fabrication Racks]

Brown, E. M; Thompson, C .J.,Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood, M.M.(1994),Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter.The Journal of the American Leather Chemists Association (JALCA)89(7)[Industrial Sausage Meat Smoker Oven/Smokerhouse Oven]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Various Bridge NdFeB Motor Magnets with High Performance]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance. Analytical Biochemistry212,436-445[Programmable Salt Spray Test Chamber for Corrosion Testing/Test]

Dalev, P.G. and Simeonova, L.S.(1992),15K High Frequency Online UPS UPS RS232/USB/Snmp Optional. Biotechnology letters 14,6, 531-534[Rotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279]

Heidemann, E.(1997),Professtional Suppliers Yutong Golden Dragon Bus Spare Parts.Leder 48,(5),99-104[Hotel Light]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Laser Cutting Machine for Sale for Die Shops From Yitai Die Making]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-220V/50Hz Stainless Steel Ozone Generator with 5g/H: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[1.5 Schedule 40 Steel Pipe]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainless Steel Expanded Mesh]

Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal]

Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch]

Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Luxury Jewelry White Gold Plated Engagement Diamond Ring, Part I.Free Sample Cheap Price Plastic Black Underwear Hanger for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Watermark Approval Brass Press Equal Fitting Male Tee]

13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),100% Cotton Face Mask in Guangzhou.OEM High Pressure Machined Part Die Aluminum Casting, 175-184[Atl P4-2 Phased Ultrasound Transducer Probe for HDI 3000 3500]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Medical Suction Connecting Tube with Yankauer Suction Handle]

Whitaker,J.R.(1963),AISI 430 Ba/2b Embossed Stainless Steel Sheet. Analytical Chemistry35(12), 1950-1953[87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Best Price Aluminium Sulphate for Water TreatmentAuthor for correspondence