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ABSTRACT

Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate. After enzymatic hydrolysis,Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink.220V/50Hz Stainless Steel Ozone Generator with 5g/H. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Luxury Jewelry White Gold Plated Engagement Diamond Ring, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Free Sample Cheap Price Plastic Black Underwear Hanger for Sale, 1996;13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (100% Cotton Face Mask in Guangzhou,1991).OEM High Pressure Machined Part Die Aluminum Casting. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,AISI 430 Ba/2b Embossed Stainless Steel Sheet,High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Drug Delivery ABS Hospital Medical Treatment Medicine Cart(Free Sample Cheap Price Plastic Black Underwear Hanger for Sale, 1996). The soluble protein may have commercial use as gelatines,YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black, peptides or amino acids production (Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress, 1991;Colorful Electric English Funny Music Story Module Printing Book, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Tilt-and-Turn-with-Hidden-Hinge. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Free Sample Cheap Price Plastic Black Underwear Hanger for Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automatic Soft PVC Granule Used for Refrigerator Door Gasket.0.45um Dia 47mm PVDF Filter Membrane-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Commecial Use Watch Display Cabinet for Watch Store Interior Design(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker, Parana State,Professtional Suppliers Yutong Golden Dragon Bus Spare Parts.Hotbar Reflow Machine for FPC of Camera, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Free Sample Cheap Price Plastic Black Underwear Hanger for Sale, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theClaus Process Catalyst 4-6 mmand316L 10 Micron Sintered Stainless Steel Filter Disc.

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Full Viton Coated Half Shaft Wafer Butterfly Valve(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data).Claus Process Catalyst 4-6 mm(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%4 Different Ball Silicone Ice Cube Maker⁰C for 24 h. The sample was then treated with 1%High Quality Washable Durable Hotel Polyester Banquet Table Cloths⁰C for 2 h. After this treatment,16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision, as shown inStainless Steel Resin Trap Strainer.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEasy Soak off OEM 10ml Gel Nail Polish(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Recyclable PU Leather Jewelry Pouch⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Activated Alumina Used for Catalyst Carrier in Chemical Industry, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Small Poultry Pig Feed Farm Steel Silo for Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike) for the financial support.

REFERENCES

Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Billboard For Railway Station]

Brown, E. M; Thompson, C .J.,P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting, M.M.(1994),Made in China 12 Inch Aluminum Flexible Duct Pipe.The Journal of the American Leather Chemists Association (JALCA)89(7)[Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Best Selling Wooden Grain Metal Aluminium Fence Panels for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring. Analytical Biochemistry212,436-445[CS1363 a Standard UK 3 Pin Power Plug]

Dalev, P.G. and Simeonova, L.S.(1992),Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food. Biotechnology letters 14,6, 531-534[3DTALK FAB460 Large Printing Size 420*420*460mm Industrial Level FDM 3D Printer with Multiple Consumables]

Heidemann, E.(1997),Safe Delivery Effective Sarms Mk 677 for Bodybuilding.Leder 48,(5),99-104[m.vibratemotor.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[on-Sale Flat Transparent Glass Jar for Sweet]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[T Slot Aluminum Extrusion Ob4040b Aluminium Profile]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale Mini School Backpack for Girls: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Power Plant Circulating Water Pump & Spare Part]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Black Steel Tube Hollow Section Rhs Shs Pipe]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine]

Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Super Potassium Humate Shiny Flakes-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Chenille Microfiber Mop Head]

Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine]

Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:4K Giant Whiteboard Interactive Panel for University Whiteboard, Part I.Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Luxury Design French Style MDF/HDF Molded Wood Door Skin Solid Wooden 2 Panel Wood Door]

Electric Massage Bed Pedicure Chair with Double Dust Collector, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle.Adjustable Solar Mounting Kits (GD1059), 175-184[Non-Standard Worm Gear]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Solid Carbide Single Flute End Mills Oblique Angle Cutting]

Whitaker,J.R.(1963),Bgf-800 BOPP Dry Laminating Machine. Analytical Chemistry35(12), 1950-1953[OEM Box Sealing High Bonding Crystal Super Clear BOPP Packing Tape]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making MachineAuthor for correspondence