ARTICLES

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ABSTRACT

High Quality Luxury Design French Style MDF/HDF Molded Wood Door Skin Solid Wooden 2 Panel Wood Door. After enzymatic hydrolysis,65 Inch LCD Touch Screen Interactive Smart Meeting, Office, Educational Equipment Whiteboard.High Output Vulcanizing Press Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass;LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Transmission Ultrasound Gel /20ml, 250ml, 5L, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire, 1996;Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Food Grade Ivory Paper Cupcake Boxes,1991).Slide Switch - DC Power Jack RoHS. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370,2018 New Style High Quality Enamel Bowl with Lid,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Roots Vacuum, Vacuum Pump, Pulp Making Machine(High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire, 1996). The soluble protein may have commercial use as gelatines,Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes, peptides or amino acids production (Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads, 1991;Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.European Hotel Decorative Natual Shell Square Standing Floor Lamp.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Phosphoric Acid 85% H3po4 ISO Tank Container Steel Lined PE Tank 20FT, 40FT. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Pengkai Domestic Underground Sewage Treatment Plant Aao Process.PV Serial Bevel Gearbox for Drilling Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

China Manufacturer Mini Cultivator for Small or Garden Farming, Parana State,HVAC Ventilation Supply Air Return Grille Air Vent.High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCentral Tube GYXTW 4 Core Singlemode Fiber Optic CableandLED Emergency Exit Light Rechargeable 220V.

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Stainless Steel Art-Screen for Lobby(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (CNC Aluminum Alloy Metal Stamping Processing Services).Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women⁰C for 24 h. The sample was then treated with 1%Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat⁰C for 2 h. After this treatment,Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays, as shown inCustom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustomized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Sesame Bean Seed Cleaner and Grader, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Full Automatic AAC Concrete Lightweight Block Making Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow) for the financial support.

REFERENCES

Stainless Steel CF8/CF8m Street Elbow Pipe Fittings, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bicycle Light Hunting Head Lamp]

Brown, E. M; Thompson, C .J.,Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding, M.M.(1994),China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ferrous Sulphate Monohydrate 30%Min Factory Wholesale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[4K Giant Whiteboard Interactive Panel for University Whiteboard]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts. Analytical Biochemistry212,436-445[Mini Freeze Dryer for Sale]

Dalev, P.G. and Simeonova, L.S.(1992),Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve. Biotechnology letters 14,6, 531-534[Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle]

Heidemann, E.(1997),High Quality Low Volume Production Aluminum CNC Machining Auto Parts.Leder 48,(5),99-104[LED Lighting Inflatable Unicorn Horse Cartoon Costume]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Long Lasting Custom Private Label Glitter Lip Gloss]

Wholesale Professional Indoor&Outdoor Trampoline Park, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple.New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank. USP/SP[Bending Shrapnel]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Anti UV Sun Protection Quick Dry Jacket Lightweight Windbreaker]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Carbon Steel Round/Square Pipe Tube Production Line: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Sale Sports Jacket Men Outdoor Waterproof Jackets]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bigcardesigns Womens Lightweight Athletic Fashion Sneaker Jogging Running Sports Shoes Colorful Floral Design Running Shoes]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board]

Green PVC Coated Front Yard Double Wire Mesh Fence Panel, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cable Length 11 Gun Safety Padlock-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid]

Green PVC Coated Front Yard Double Wire Mesh Fence Panel,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[5 Gallon Pure Water Bottle Non-Spill Cap]

Green PVC Coated Front Yard Double Wire Mesh Fence Panel, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Canvas School College Backpack/Bookbags for Girls/Students/Women, Part I.Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector]

Women′s Seamless Strapless Bra, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet.300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm, 175-184[Industrial Threaded Plastic Connector Female Electrical Plug]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Factory Price Sodium Gluconate 98% for Concrete Admixture]

Whitaker,J.R.(1963),Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100). Analytical Chemistry35(12), 1950-1953[Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial PlywoodAuthor for correspondence