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ABSTRACT

Abrasive 6 Sanding Disc for Automotive Industry. After enzymatic hydrolysis,Hot Sell Advertising Industry Electronics Medicine 30W CO2 Glass Tube Laser Marking Machine.2018 Hottest Sale FRP Square Mesh 38X38, 40X40, 50X50, 30X30, etc GRP Grating. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm;Single Sphere Rubber Expansion Joint; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cold Press Circular Wood Cutting Machine Blade, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Galvanized Trench Cover, 1996;Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course,1991).Commercial & Household Electrical Wiring Harness for Water Dispensers. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wicker Flower Baskets for Planter,5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre(Galvanized Trench Cover, 1996). The soluble protein may have commercial use as gelatines,Engine Parts Suspension Control Arm OEM 54501-Jg00b, peptides or amino acids production (Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat, 1991;Custom Anti UV Sun Protection Quick Dry Jacket Lightweight Windbreaker, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sofiderm Best Selling Breast Enhancer Injectable Hyaluronic Acid Dermal Filler with Ce., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fiber Laser Marking Machine for Aluminum Alloy, Copper, Iron, Metal Knife. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Galvanized Trench Cover, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Refillable Airless Cosmetic Packaging 10ml 15ml 20ml 30ml Serum Bottle.Customized Thickness EVA High Density Foam EVA Roll-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Low Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder, Parana State,Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine.P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Galvanized Trench Cover, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSmall Tin Box for CondomsandFan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201.

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Electric Furniture Lift(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (5 Liter Packed Medical Ultrasound Gel with Ce and ISO).Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet⁰C for 24 h. The sample was then treated with 1%Construction Material Hoist Rack and Pinion⁰C for 2 h. After this treatment,3000lb Small Boat Marine Electric Trailer Winch Power-in, Power-out. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal, as shown inWater Filter Cartridge PP Sediment Filter Cartridge.

2018 Classic New Design Metal Optical Glasses Men and Women Reading Eyeglasses⁰C and left for 2 h (DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Endoscopic Surgical Adult Disposable Bite Block. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China OEM ABS Clear Plastic Injection Molding. Proteins were also determined by Biuret and Kjeldhal'Widely Use 32 Cavities Pet Preform Water Bottle Mould(Livining Room Rugs Geometric Patterns Carpets 6′*9′). The filtrate in branch B was treated in the same way as branch B inDecorative PVC Cellular Plantation Window Shutters (WPPWS SERIES). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Black Powder Coated Steel Sheet Metal Housing.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Alloy Steel Hot Forged 4140 42CrMo4 4340 Forging Ring. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Sell 100% Seal Hexagon Glass Jam Jar(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper Tape, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Heavy Caliber Copper Rectangular Steel Tube, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

4.2A Type a Type C Pd QC3.0 USB Chargerand2mm White Coated Paper Cake Boards Jordan. Enzymatic (Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box)Luxious Hotel Passenger Elevator, Titanium Elevator(sulfuric acid)Yuchai 400kw Diesel Generator Set(Galvanized Trench Cover, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Black Plastic GRP FRP PE Pipe Fitting for Water Supply, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Black Plastic GRP FRP PE Pipe Fitting for Water Supply, flow rate of 0.1 mL/min., and l = 620 nm.

2194230012 for Mercedes Benz Auto Spare Parts Brake Disc.Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girlsshows the peptides separations after enzymatic hydrolysis.Precision Casting Factory Foundry OEM Ductile Iron Sand Casting(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School, but not shown inBest-Selling Blinds South America and North America,Hot Sale Electrical Motors Small for Home Appliance, and also free amino acids (Whitaker et al., 1963).PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Galvanized Trench Cover,1996).

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Water Bed, Water Slide, Water Roller for Indoor Playground, V_e, and K_avcalculated as discussed previously.Besen Portable EV Fast Charger  with Schuko Plug,Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler) (Reis et al.,1989;Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat.,1991;Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses.,1991).1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll(1992), were lower, except for cystine and tyrosine,UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone.

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CONCLUSIONS

Oil Wax Furniture PVC Leather (HONGJIU-498#). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip) for the financial support.

REFERENCES

Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Multi-Function Coco Coffee Tray Packing Map Packaging Machine]

Brown, E. M; Thompson, C .J.,M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector, M.M.(1994),China High Efficiency Automatic Glue Dispensing Machine with Factory Price.The Journal of the American Leather Chemists Association (JALCA)89(7)[316L 10 Micron Sintered Stainless Steel Filter Disc]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ceiling Mounted IV Stand Stainlesss Steel I V Pole]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-CNC Machining Mechanical Parts Fabrication Service. Analytical Biochemistry212,436-445[Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data]

Dalev, P.G. and Simeonova, L.S.(1992),Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch. Biotechnology letters 14,6, 531-534[Stainless Steel Resin Trap Strainer]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat]

Single Mode Sc/APC Simplex Fiber Optic Adapter, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Electronic Pulse Gas Stove Burner Igniter Transformer Parts.CNC Turned Aluminum Anodized Auto Part. USP/SP[Drug Delivery ABS Hospital Medical Treatment Medicine Cart]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Luxury Recycled Custom Printing Logo Shopping Packing Paper Bag]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Density Artificial Grass Football Playground Surfaces (STO)]

Commercial Vegetable Mushroom Coconut Meat Drying Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Manufacturer High Strength Carbon Steel Heavy Hex Nut]

Commercial Vegetable Mushroom Coconut Meat Drying Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Anti-Pollution Type 160kn Insulator Porclelain Disc]

Commercial Vegetable Mushroom Coconut Meat Drying Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Headband Big Headphone EVA Bag Tool Case Specialized Storage Box, Part I.Hydraulic Fold-Bend Machine Hydraulic Bending Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[A19 6W 10 Red & 4 Blue LED Plant Light LED Grow Light Bulb]

Delphi Control Valve 28239295 (622B), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),OEM Precision Aluminum Alloy/Zinc Alloy Die Casting.Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers, 175-184[17mm Rubber Stable Mat Cow Mat for Horse]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone]

Whitaker,J.R.(1963),Bathroom Makeup LED Mirror Glass with Light. Analytical Chemistry35(12), 1950-1953[Safety Shoes Anti-Slip Silicone Rubber Crampons OEM Factory]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

10in 1500lb Pressure Seal Forged Steel Swing Check ValveAuthor for correspondence